HCT MIDTERM PRACTICALS EXAM QUESTIONS AND ANSWERS
Exercise 1 - CORRECT ANSWER✅✅Supplies & Equipment
Rotary Microtome - CORRECT ANSWER✅✅Use: Thin Slicing of Tissue
Maintain: Remove excess paraffin,
Apply oil in moving parts
Cryostat Microtome - CORRECT ANSWER✅✅Use: Frozen Sectioning
Maintain: Remove excess paraffin,
Temp. at -20 degrees
Automated Tissue
Embedding System - CORRECT ANSWER✅✅Use: Automated embedding &
cooling of tissues
Maintain: Temp. of cold plate,
Clean embedding mold
Automated Tissue Processor - CORRECT ANSWER✅✅Use: Automatic tissue processing
Maintain: Clean beakers,
Change sol'n once a week
Binocular Light Microscope - CORRECT ANSWER✅✅Use: Microscopic examination of tissue
Maintain: Clean oil when using OIO
, Microtome Knife w/ Adaptor - CORRECT ANSWER✅✅Use: Cutting tissue section
Maintain: Sharpen dull ends if reusable
Plastic Tissue Cassette - CORRECT ANSWER✅✅Use: Holding & identifying tissues
Maintain: Wash and dry,
Remove Excess Paraffin
Glass Slides w/ Cover Slips - CORRECT ANSWER✅✅Use: Tissue samples are mounted
for examination
Maintain: Disposable
Teasing Needle / Probe - CORRECT ANSWER✅✅Use: Tease folds in tissue
Maintain: Clean / wipe to prevent rusting
Tissue Floatation Bath - CORRECT ANSWER✅✅Use: Stretch sections,
remove wrinkles and folds
Maintain: Free from oil & albumin,
check temp: 45-50C
Staining Dish & Slide Carrier - CORRECT ANSWER✅✅Use: Holding slides during staining
Maintain: Clean with soap water,
Exercise 1 - CORRECT ANSWER✅✅Supplies & Equipment
Rotary Microtome - CORRECT ANSWER✅✅Use: Thin Slicing of Tissue
Maintain: Remove excess paraffin,
Apply oil in moving parts
Cryostat Microtome - CORRECT ANSWER✅✅Use: Frozen Sectioning
Maintain: Remove excess paraffin,
Temp. at -20 degrees
Automated Tissue
Embedding System - CORRECT ANSWER✅✅Use: Automated embedding &
cooling of tissues
Maintain: Temp. of cold plate,
Clean embedding mold
Automated Tissue Processor - CORRECT ANSWER✅✅Use: Automatic tissue processing
Maintain: Clean beakers,
Change sol'n once a week
Binocular Light Microscope - CORRECT ANSWER✅✅Use: Microscopic examination of tissue
Maintain: Clean oil when using OIO
, Microtome Knife w/ Adaptor - CORRECT ANSWER✅✅Use: Cutting tissue section
Maintain: Sharpen dull ends if reusable
Plastic Tissue Cassette - CORRECT ANSWER✅✅Use: Holding & identifying tissues
Maintain: Wash and dry,
Remove Excess Paraffin
Glass Slides w/ Cover Slips - CORRECT ANSWER✅✅Use: Tissue samples are mounted
for examination
Maintain: Disposable
Teasing Needle / Probe - CORRECT ANSWER✅✅Use: Tease folds in tissue
Maintain: Clean / wipe to prevent rusting
Tissue Floatation Bath - CORRECT ANSWER✅✅Use: Stretch sections,
remove wrinkles and folds
Maintain: Free from oil & albumin,
check temp: 45-50C
Staining Dish & Slide Carrier - CORRECT ANSWER✅✅Use: Holding slides during staining
Maintain: Clean with soap water,
