LS7C FINAL EXAM
,1. To determine if Aeromonas and Vibrio can co-exist in the Zebrafish
gut you colonize germ free zebrafish larva with Aeromonas first and
then expose the Zebrafish+Aeromonas to Vibrio.You sample and count
the number of bacteria present in the gut at multiple times throughout
the experiment. The results from your experiment are shown in log
scale below:
A. Aeromonas and Vibrio can persistently co-exist in the larval z
brafish gut.
Vibrio
B. In the larval zebrafish gut, Aeromonasare able to out compete be-
cause
they were the first colonizers.
C. Vibrio are able to out compete Aeromonas in the larval zebrafish
gut.
D. Vibrio are able to utilize food sources present in the larval zebrafish
gut better than Aeromonas.: C. Vibrio are able to out compete Aeromonas
in the larval zebrafish gut.
2. You are curious about the mechanism of competitive exclusion
present in the larval zebrafish gut so you fluorescently label Vibrio and
Aeromonasand run the same colonization challenge experiment as
before but measure the presence of each species over a smaller time
scale (hours vs. days). The results from your experiment are shown
below.
,Based on the interpretation of the data provided, we can conclude: A.
In the larval zebrafish gut, Aeromonasare able to out compete
cause they were the first colonizers. ibriobe-
rafish gut
B. Vibrio are able to utilize food sources present in the larval zeb better
than Aeromonas.
C. Vibrio are able to out compete Aeromonas in the larval zebrafish gut
because the zebrafish host selectively eliminates only Aeromonas.
D. Vibrio are able to out compete Aeromonas in the larval zebrafish gut
because Vibrio are resistant to intestinal contractions: D. Vibrio are able
to
out compete Aeromonas in the larval zebrafish gut because Vibrio are
resistant to intestinal contractions
, 3. True or false: 16S rRNA gene sequencing utilizes RNA to identify
microbial species present in a microbiota sample.: False - utilizes the
genes (DNA) that encode small ribosomal subunits (RNA)
4. Based on the data provided, how many more unique OTUs were
identified in sample 1 compared to sample 5?
A. 1
B. 2
C. 3
D.4
E. 5: C. 3
5. Which graph best represents these data? (OTUs)
A. Graph A
B. Graph B: B. Graph B
6. The sequencing data below allows you to determine that is
respon- sible for the variance in microbial diversity between samples.
A. Diet
B. Age
C. Health status
D. Cannot be determined: D. Cannot be determined
7. Bacterial communities present in samples from oral body regions are
of the body.
similar to bacterial communities from different regions
,1. To determine if Aeromonas and Vibrio can co-exist in the Zebrafish
gut you colonize germ free zebrafish larva with Aeromonas first and
then expose the Zebrafish+Aeromonas to Vibrio.You sample and count
the number of bacteria present in the gut at multiple times throughout
the experiment. The results from your experiment are shown in log
scale below:
A. Aeromonas and Vibrio can persistently co-exist in the larval z
brafish gut.
Vibrio
B. In the larval zebrafish gut, Aeromonasare able to out compete be-
cause
they were the first colonizers.
C. Vibrio are able to out compete Aeromonas in the larval zebrafish
gut.
D. Vibrio are able to utilize food sources present in the larval zebrafish
gut better than Aeromonas.: C. Vibrio are able to out compete Aeromonas
in the larval zebrafish gut.
2. You are curious about the mechanism of competitive exclusion
present in the larval zebrafish gut so you fluorescently label Vibrio and
Aeromonasand run the same colonization challenge experiment as
before but measure the presence of each species over a smaller time
scale (hours vs. days). The results from your experiment are shown
below.
,Based on the interpretation of the data provided, we can conclude: A.
In the larval zebrafish gut, Aeromonasare able to out compete
cause they were the first colonizers. ibriobe-
rafish gut
B. Vibrio are able to utilize food sources present in the larval zeb better
than Aeromonas.
C. Vibrio are able to out compete Aeromonas in the larval zebrafish gut
because the zebrafish host selectively eliminates only Aeromonas.
D. Vibrio are able to out compete Aeromonas in the larval zebrafish gut
because Vibrio are resistant to intestinal contractions: D. Vibrio are able
to
out compete Aeromonas in the larval zebrafish gut because Vibrio are
resistant to intestinal contractions
, 3. True or false: 16S rRNA gene sequencing utilizes RNA to identify
microbial species present in a microbiota sample.: False - utilizes the
genes (DNA) that encode small ribosomal subunits (RNA)
4. Based on the data provided, how many more unique OTUs were
identified in sample 1 compared to sample 5?
A. 1
B. 2
C. 3
D.4
E. 5: C. 3
5. Which graph best represents these data? (OTUs)
A. Graph A
B. Graph B: B. Graph B
6. The sequencing data below allows you to determine that is
respon- sible for the variance in microbial diversity between samples.
A. Diet
B. Age
C. Health status
D. Cannot be determined: D. Cannot be determined
7. Bacterial communities present in samples from oral body regions are
of the body.
similar to bacterial communities from different regions
