MICROBIOLOGY EXAM 1 2025| COMPLETE EXAM SET
(QUESTIONS AND VERIFIED ANSWERS)FREQUENTLY MOST
TESTED QUESTIONS | ALREADY PASSED!!
Question 1
What is another name for Rothia mucilaginosus (Stomatococcus)?
A) Green Staph
B) Shiny Staph
C) Dry Staph
D) "Sticky Staph"
E) Mutant Staph
Correct Answer: D) "Sticky Staph"
Rationale: Rothia mucilaginosus (Stomatococcus) is also known as "Sticky Staph."
Question 2
Which chemical agent is recommended for cleaning blood spills, after initial cleaning with soap
and water?
A) Alcohol-based hand sanitizer
B) Hydrogen peroxide
C) 1/10 dilution of bleach (NaClO)
D) Quaternary ammonium compound
E) Iodine solution
Correct Answer: C) 1/10 dilution of bleach (NaClO)
Rationale: For blood spills, "1/10 dilution of bleach (NaClO); clean 1st (soap & water)
& then disinfect" is recommended.
Question 3
At what temperature does agar media typically melt?
,A) 45-50 degrees C
B) 25 degrees C
C) 37 degrees C
D) 100 degrees C
E) 15 psi
Correct Answer: D) 100 degrees C
Rationale: Agar media melts at "100 degrees C."
Question 4
At what temperature does agar media solidify or stay liquid until?
A) 100 degrees C
B) 37 degrees C
C) 25 degrees C
D) 45-50 degrees C
E) 15 minutes
Correct Answer: D) 45-50 degrees C
Rationale: Agar media "stays liquid until 45-50o C (or solidifies at 45-50o C)."
Question 5
What are the appropriate temperature and pressure settings when autoclaving material for
sterilization?
A) 15 psi for 100 C degrees for 15 minutes
B) 10 psi for 121 C degrees for 30 minutes
C) 20 psi for 115 C degrees for 20 minutes
D) 15 psi for 121 C degrees for 15 minutes
, E) 10 psi for 100 C degrees for 10 minutes
Correct Answer: D) 15 psi for 121 C degrees for 15 minutes
Rationale: The appropriate temperature and pressure when autoclaving material are
"15 psi for 121 C degrees for 15 minutes."
Question 6
What is the optimal incubation temperature for most bacteria commonly encountered in a
clinical microbiology laboratory?
A) 22-25 degrees C
B) 42 degrees C
C) 55 degrees C
D) 35 to 37 degrees C
E) 100 degrees C
Correct Answer: D) 35 to 37 degrees C
Rationale: The optimal incubation temperature for most bacteria is "35 to 37
degrees C (body temperature)."
Question 7
Which of the following is NOT one of the three most common temperatures used in the
laboratory to isolate bacteria?
A) 35-37 degrees C
B) 22-25 degrees C
C) 42 degrees C
D) 98 degrees C
E) All are common temperatures.
(QUESTIONS AND VERIFIED ANSWERS)FREQUENTLY MOST
TESTED QUESTIONS | ALREADY PASSED!!
Question 1
What is another name for Rothia mucilaginosus (Stomatococcus)?
A) Green Staph
B) Shiny Staph
C) Dry Staph
D) "Sticky Staph"
E) Mutant Staph
Correct Answer: D) "Sticky Staph"
Rationale: Rothia mucilaginosus (Stomatococcus) is also known as "Sticky Staph."
Question 2
Which chemical agent is recommended for cleaning blood spills, after initial cleaning with soap
and water?
A) Alcohol-based hand sanitizer
B) Hydrogen peroxide
C) 1/10 dilution of bleach (NaClO)
D) Quaternary ammonium compound
E) Iodine solution
Correct Answer: C) 1/10 dilution of bleach (NaClO)
Rationale: For blood spills, "1/10 dilution of bleach (NaClO); clean 1st (soap & water)
& then disinfect" is recommended.
Question 3
At what temperature does agar media typically melt?
,A) 45-50 degrees C
B) 25 degrees C
C) 37 degrees C
D) 100 degrees C
E) 15 psi
Correct Answer: D) 100 degrees C
Rationale: Agar media melts at "100 degrees C."
Question 4
At what temperature does agar media solidify or stay liquid until?
A) 100 degrees C
B) 37 degrees C
C) 25 degrees C
D) 45-50 degrees C
E) 15 minutes
Correct Answer: D) 45-50 degrees C
Rationale: Agar media "stays liquid until 45-50o C (or solidifies at 45-50o C)."
Question 5
What are the appropriate temperature and pressure settings when autoclaving material for
sterilization?
A) 15 psi for 100 C degrees for 15 minutes
B) 10 psi for 121 C degrees for 30 minutes
C) 20 psi for 115 C degrees for 20 minutes
D) 15 psi for 121 C degrees for 15 minutes
, E) 10 psi for 100 C degrees for 10 minutes
Correct Answer: D) 15 psi for 121 C degrees for 15 minutes
Rationale: The appropriate temperature and pressure when autoclaving material are
"15 psi for 121 C degrees for 15 minutes."
Question 6
What is the optimal incubation temperature for most bacteria commonly encountered in a
clinical microbiology laboratory?
A) 22-25 degrees C
B) 42 degrees C
C) 55 degrees C
D) 35 to 37 degrees C
E) 100 degrees C
Correct Answer: D) 35 to 37 degrees C
Rationale: The optimal incubation temperature for most bacteria is "35 to 37
degrees C (body temperature)."
Question 7
Which of the following is NOT one of the three most common temperatures used in the
laboratory to isolate bacteria?
A) 35-37 degrees C
B) 22-25 degrees C
C) 42 degrees C
D) 98 degrees C
E) All are common temperatures.
