MICR 4423 EXAM 6 QUESTIONS AND
CORRECT ANSWERS!!
what is antibiotic selection?
the most resistant cells surviving when antibiotic resistance is around
where can abx selection occur?
anywhere that bacteria and abx is present
name a common use for abx selection in microbiological research
identifying particular cells that contain a DNA of interest
-ex: plasma, transform cells w a plasma and plate them on an agar
Why does virtually every plasmid used for scientific research contain an antibiotic
resistance marker?
so you can identify the cells that have the plasma
imagine that you have a sample of bacteria that is a mixture of ampicillin-resistant cells
and ampicillin-sensitive cells. how could you isolate the ampicillin-resistant cells?
plate on a medium with ampicillin, the ones that grow are resistant
what antibiotic or class of antbiotics do cells containing the bla marker become resistant
to?
ampicillin
what antibiotic or class of antbiotics do cells containing the aac marker become resistant
to?
kanamycin
what antibiotic or class of antbiotics do cells containing the cat marker become resistant
to?
chloramphenicol
, what is the Tet-ON and tet-OFF systems typically used for?
the induced gene expression in eukaryotic systems
what is a typical inducer of the Tet-ON or tet-OFF system?
doxycycline
Tet-ON with TET:
makes the repressor leave the operator
TET-ON without tet:
repressor binds to operator and no transcription occurs
TET-OFF without tet:
transactivation binds to the repressor and allows transcription to occur
TET-OFF with TET:
the TA and repressor bind and leave the operator
what is a typical way of administering Tet/dox to an animal
add to drinking water
how are abx regulated in the U.S compared to northern Europe:
U.S: prescription, pharmacy doctor
other countries: over the counter
Pro's of having abx availible by Rx only:
-Dr. prescribes the right Abx and course first time
-less risk of resistance/encouraging it
-choice of ABX + compliance
CORRECT ANSWERS!!
what is antibiotic selection?
the most resistant cells surviving when antibiotic resistance is around
where can abx selection occur?
anywhere that bacteria and abx is present
name a common use for abx selection in microbiological research
identifying particular cells that contain a DNA of interest
-ex: plasma, transform cells w a plasma and plate them on an agar
Why does virtually every plasmid used for scientific research contain an antibiotic
resistance marker?
so you can identify the cells that have the plasma
imagine that you have a sample of bacteria that is a mixture of ampicillin-resistant cells
and ampicillin-sensitive cells. how could you isolate the ampicillin-resistant cells?
plate on a medium with ampicillin, the ones that grow are resistant
what antibiotic or class of antbiotics do cells containing the bla marker become resistant
to?
ampicillin
what antibiotic or class of antbiotics do cells containing the aac marker become resistant
to?
kanamycin
what antibiotic or class of antbiotics do cells containing the cat marker become resistant
to?
chloramphenicol
, what is the Tet-ON and tet-OFF systems typically used for?
the induced gene expression in eukaryotic systems
what is a typical inducer of the Tet-ON or tet-OFF system?
doxycycline
Tet-ON with TET:
makes the repressor leave the operator
TET-ON without tet:
repressor binds to operator and no transcription occurs
TET-OFF without tet:
transactivation binds to the repressor and allows transcription to occur
TET-OFF with TET:
the TA and repressor bind and leave the operator
what is a typical way of administering Tet/dox to an animal
add to drinking water
how are abx regulated in the U.S compared to northern Europe:
U.S: prescription, pharmacy doctor
other countries: over the counter
Pro's of having abx availible by Rx only:
-Dr. prescribes the right Abx and course first time
-less risk of resistance/encouraging it
-choice of ABX + compliance
