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A nanometer is defined as: - answer-10 -9 or one-billionth of a meter
True or False: A nanometer is longer than a micrometer - answer-False
A nanometer is 1000x smaller than a micrometer
Resolution and contrast are two critical factors that influence your ability to
see an object. Explain each. - answer-Resolution refers to the distance
between two objects at which the objects can still be seen as separate. Poor or
low resolution means two or more objects may appear as one.
Contrast on the other hand is the difference in light absorbance between two
objects. Poor contrast gives a high background and makes the visualization of
multiple objects difficult. For instance, trying to identify 2 dark colored objects
at
night (low light = low contrast) versus the same 2 objects in the middle of
a sunny afternoon (bright light against 2 dark objects = high contrast).
, What is the total magnification on (relative to your eye) of a sample imaged
with a 60x objective and a 10x eyepiece? Show your math. - answer-60x
objective x 10x ocular = 600x magnification
True or False: Staining is often required to image a cell that is adherent and flat
(thin). - answer-True
Adherent, flat and unstained cells are almost invisible due to the limits on both
resolution and contrast. Therefore, cell staining is often required to adequately
image
the sample.
True or False: A cell that is adherent, flat (thin), and unstained is easily
identified using bright field microscopy - answer-False: Adherent, flat cells are
almost invisible due to the limits on both resolution and contrast
Which of the following could be seen clearly by the unaided eye? Select all that
apply.
A. Bacteria with diameter of 24 μm
B. Protozoa with diameter of 150 μm
C. Virus with a diameter of 0.2 μm
D. Skin cell with diameter of 1500 μm - answer-B. Protozoa with diameter of
150 μm
D. Skin cell with diameter of 1500 μm