CHEM 219
Lab Report & Lab Notes
Experiment #: 3 Extraction
Portage Learning
Inside you will get:
- Clear, Concise Scientific Language.
- All Tables and Figures Labelled Clearly.
- Included Units for All Measurements.
- Proofread Report.
- Portage Learning.
, Chemistry Lab Report
Experiment #: 3
Title: Extraction
Purpose:
The experiment aims to use extraction to isolate caffeine from commercially available
tea bags. This will show the technique commonly used in organic chemistry to isolate a
target compound.
Procedure:
1. Add 15 mL of boiling water to a beaker and 2 grams of sodium carbonate (slightly
basic) to counteract the acidity of the tea. Let it dissolve in the water on the hot
plate
2. Shake the tea to the bottom of the bag and wrap it up using the string so it fits in
the beaker. Place the tea bag in the solution and steep it for 5 minutes. Add small
quantities of water to make sure the tea bag remains submerged
3. Remove the tea bag from the water and gently squeeze all the liquid from the
bag
4. Repeat the process with the second tea bag
5. Transfer the tea solution into a centrifuge tube (15mL) and make sure the volume
of tea does not go past 12 mL. The solvent has a boiling point of 40 degrees so
cool the tea concentration in ice.
6. Add 2mL of methylene chloride into the tube. Shake the tube (rock back and
forth) to mix the liquids and stop occasionally to open the cap and release any
pressure buildup then put the centrifuge back into the holder and allow the liquids
to separate.
7. Use the centrifuge to break up the emulsion layer between the two liquids. Spin
at 50,000 rpm for 3 minutes.
8. Use a pipette at the bottom of the tube to withdraw the methylene chloride layer
and transfer is to the flask
9. Repeat the extraction step 2 more times using 2 mL of methylene chloride.
10. Dry the methylene chloride by adding a small quantity of sodium sulfate (absorbs
water, does not dissolve in organic solvents). Add small amounts at a time and
swirl. Tea gets absorbed by sodium sulfate and begins to clump. Let it sit for 5
minutes. Transfer the mixture into a pre-weighed flask to preform evaporation.
Wash drying agent with small quantity of solvent to ensure all caffeine is
removed
11. Transfer the pre-weighed flask to a hot plate and allow the mixture to slowly
evaporate.
12. Loosen up the solid caffeine from bottom of the flask and grind it with the
scoopula. Pack a small amount into the melting point capillary and pack it into the
This study source
/Chem219Experiment3docx/
Lab Report & Lab Notes
Experiment #: 3 Extraction
Portage Learning
Inside you will get:
- Clear, Concise Scientific Language.
- All Tables and Figures Labelled Clearly.
- Included Units for All Measurements.
- Proofread Report.
- Portage Learning.
, Chemistry Lab Report
Experiment #: 3
Title: Extraction
Purpose:
The experiment aims to use extraction to isolate caffeine from commercially available
tea bags. This will show the technique commonly used in organic chemistry to isolate a
target compound.
Procedure:
1. Add 15 mL of boiling water to a beaker and 2 grams of sodium carbonate (slightly
basic) to counteract the acidity of the tea. Let it dissolve in the water on the hot
plate
2. Shake the tea to the bottom of the bag and wrap it up using the string so it fits in
the beaker. Place the tea bag in the solution and steep it for 5 minutes. Add small
quantities of water to make sure the tea bag remains submerged
3. Remove the tea bag from the water and gently squeeze all the liquid from the
bag
4. Repeat the process with the second tea bag
5. Transfer the tea solution into a centrifuge tube (15mL) and make sure the volume
of tea does not go past 12 mL. The solvent has a boiling point of 40 degrees so
cool the tea concentration in ice.
6. Add 2mL of methylene chloride into the tube. Shake the tube (rock back and
forth) to mix the liquids and stop occasionally to open the cap and release any
pressure buildup then put the centrifuge back into the holder and allow the liquids
to separate.
7. Use the centrifuge to break up the emulsion layer between the two liquids. Spin
at 50,000 rpm for 3 minutes.
8. Use a pipette at the bottom of the tube to withdraw the methylene chloride layer
and transfer is to the flask
9. Repeat the extraction step 2 more times using 2 mL of methylene chloride.
10. Dry the methylene chloride by adding a small quantity of sodium sulfate (absorbs
water, does not dissolve in organic solvents). Add small amounts at a time and
swirl. Tea gets absorbed by sodium sulfate and begins to clump. Let it sit for 5
minutes. Transfer the mixture into a pre-weighed flask to preform evaporation.
Wash drying agent with small quantity of solvent to ensure all caffeine is
removed
11. Transfer the pre-weighed flask to a hot plate and allow the mixture to slowly
evaporate.
12. Loosen up the solid caffeine from bottom of the flask and grind it with the
scoopula. Pack a small amount into the melting point capillary and pack it into the
This study source
/Chem219Experiment3docx/
