BCH4024 EXAM 1 REVISED
QUESTIONS WITH CORRECT
DETAILED ANSWERS
Limited size of active sites (stereospecific) keeps binding energy in a ---------, perfect
state - Answer-Goldilocks state
Chymotrypsin tends to cleave -------chains. And --------glucose is the most common
sugar - Answer-long, D
----------- transition state in enzyme catalysis means that substrate and enzyme change
simultaneously - Answer-Joint
The joint transition state is also very ------------, lasting for very short period of time -
Answer-transient
The active site of the enzyme will almost always do everything it can to -----------
reactivity - Answer-promote
Enzymes love to make work easier, they like to go through the -------of the energy
barriers, they are therefore said to be ------- - Answer-valleys, syncatalytic
In an uncatalyzed reaction, the delta G is --------- than zero - Answer-greater
In an enzyme catalyzed reaction, forward and backwards rates increase by the -----------
-factor - Answer-same
Velocity of a reaction is defined as the concentration change per - Answer-second
For first order reaction, velocity depends only on ---------OR ------ - Answer-reactants,
products
----------theory talks about reaction rate in relation to ------------barrier height - Answer-
Absolute, energy
The higher the energy barrier, the slower the --------of the reaction - Answer-rate
The ---------frequency of a chemical bond is the fastest that a rxn can go - Answer-
vibrational
The larger the delta G, the slower the -------- - Answer-rate constant (k)
, --------energy is the energy released in the substrate enzyme interaction - Answer-
Binding
Substrate specificity relies on ----------interactions, which in the transition state are --------
--than the ES complex - Answer-noncovalent, stronger
The specificity of ES binding depends on the ----------structure - Answer-tertiary
If substrates were completely complementary to their enzymes, lock and key, the
reaction would be stuck at ---------energy ES - Answer-low
The active site of the enzyme is complementary to the --------- - Answer-transition state
Transition state analogs are ------inhibitors - Answer-good
Enzymes can promote catalysis by forming -----------bonds, which are transient -
Answer-covalent
Most of the time nucleophiles have a ----------character and electrophiles have a ----------
-character - Answer-negative, positive
Enzymes will orient substrates as to achieve the fastest reaction rate, for nucleophilic
reactions, the SN2 chemistry may be needed. As the lg leave the electrophile may -------
--itself to take on the new incoming - charged nucleophile - Answer-reorient
transient covalent bonding in ES helps to preserve ------------during catalysis. AA's like
lys, his, cys, asp, thr, ser may be modified - Answer-chemical bond
Metal ions is like mg, mn, zn , different version o fe can act as lewis -------- (e acceptor)
and ----------to bind and orient redox reactions and always ------the negative charge in
the transition state - Answer-acids, templates, stabilize
Metal ions also increase the --------- of polar molecules and mediate redox reactions -
Answer-acidity
Serine is a terrible nucleophile, the enzyme ---------makes it better - Answer-
chymotrypsin
It is important to remember again that chymotrypsin cleaves proteins on the -----terminal
- Answer-C
The first product cleaved by chymotrypsin has the -----terminal fragment, meaning the
side with the NH3+ - Answer-C
QUESTIONS WITH CORRECT
DETAILED ANSWERS
Limited size of active sites (stereospecific) keeps binding energy in a ---------, perfect
state - Answer-Goldilocks state
Chymotrypsin tends to cleave -------chains. And --------glucose is the most common
sugar - Answer-long, D
----------- transition state in enzyme catalysis means that substrate and enzyme change
simultaneously - Answer-Joint
The joint transition state is also very ------------, lasting for very short period of time -
Answer-transient
The active site of the enzyme will almost always do everything it can to -----------
reactivity - Answer-promote
Enzymes love to make work easier, they like to go through the -------of the energy
barriers, they are therefore said to be ------- - Answer-valleys, syncatalytic
In an uncatalyzed reaction, the delta G is --------- than zero - Answer-greater
In an enzyme catalyzed reaction, forward and backwards rates increase by the -----------
-factor - Answer-same
Velocity of a reaction is defined as the concentration change per - Answer-second
For first order reaction, velocity depends only on ---------OR ------ - Answer-reactants,
products
----------theory talks about reaction rate in relation to ------------barrier height - Answer-
Absolute, energy
The higher the energy barrier, the slower the --------of the reaction - Answer-rate
The ---------frequency of a chemical bond is the fastest that a rxn can go - Answer-
vibrational
The larger the delta G, the slower the -------- - Answer-rate constant (k)
, --------energy is the energy released in the substrate enzyme interaction - Answer-
Binding
Substrate specificity relies on ----------interactions, which in the transition state are --------
--than the ES complex - Answer-noncovalent, stronger
The specificity of ES binding depends on the ----------structure - Answer-tertiary
If substrates were completely complementary to their enzymes, lock and key, the
reaction would be stuck at ---------energy ES - Answer-low
The active site of the enzyme is complementary to the --------- - Answer-transition state
Transition state analogs are ------inhibitors - Answer-good
Enzymes can promote catalysis by forming -----------bonds, which are transient -
Answer-covalent
Most of the time nucleophiles have a ----------character and electrophiles have a ----------
-character - Answer-negative, positive
Enzymes will orient substrates as to achieve the fastest reaction rate, for nucleophilic
reactions, the SN2 chemistry may be needed. As the lg leave the electrophile may -------
--itself to take on the new incoming - charged nucleophile - Answer-reorient
transient covalent bonding in ES helps to preserve ------------during catalysis. AA's like
lys, his, cys, asp, thr, ser may be modified - Answer-chemical bond
Metal ions is like mg, mn, zn , different version o fe can act as lewis -------- (e acceptor)
and ----------to bind and orient redox reactions and always ------the negative charge in
the transition state - Answer-acids, templates, stabilize
Metal ions also increase the --------- of polar molecules and mediate redox reactions -
Answer-acidity
Serine is a terrible nucleophile, the enzyme ---------makes it better - Answer-
chymotrypsin
It is important to remember again that chymotrypsin cleaves proteins on the -----terminal
- Answer-C
The first product cleaved by chymotrypsin has the -----terminal fragment, meaning the
side with the NH3+ - Answer-C
