ASCP CG Certification Practice
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Questions with Clear Correct
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2026)| 100% Guaranteed Success.
What is the best agent to use against mycoplasma? - Answer✅✅BM cyclin - a
combination of two antibiotics (tiamulin and minocycline) that effecticely targets
mycoplasma growth
For successful harvest, each new batch of hypotonic solution should be evaluated by:
- Answer✅✅comparing the new lot to a lot already in use
Colagenase, pronase, and trypsin are: - Answer✅✅Proteolytic enzymes - break
down tissues into single-cell suspensions
When harvesting peripheral blood cultures, asceptic technique no longer matters
following incubation with: - Answer✅✅Colcemid - mitotic spindle poison that halts
cells at metaphase
Samples that arrive at the cytogenetics lab should first be: - Answer✅✅Logged in to
the system
,Phytohemagglutinin (PHA) stimulates which type of cells into division? -
Answer✅✅T-cell lymphocytes
To avoid culture failure, monitoring reagents is crucial. When logging a culture
failure, what is the MOST important to document? - Answer✅✅Expiration dates of
reagents used
Which type of microscope is used when cleaning a CVS (chorionic villus) sample? -
Answer✅✅Dissection
Which cell type is most problematic if found in a CVS (chorionic villus) sample? -
Answer✅✅Decidua (maternal tissue)
When looking at a slide under a phase contrast scope and a lot of cytoplasm is still
seen around the mets, what is the most likely cause? - Answer✅✅innappropriate
slide drying time
What will help prevent microbial contamination and cross contamination of cultures?
- Answer✅✅Asceptic technique
When there are not enough mets to complete the study of a patient/case, what
needs to be done next? - Answer✅✅prepare additional slides from any remaining
sample
When looking at a dropped slide under a phase scope and mets are very spread out
and sparse, what could help fix the issue? - Answer✅✅Increading the drying time
by using warm slides
What is true of an inverted microscope? - Answer✅✅the objectives are beneath
the stage
High-resolution banding is indicated for a possible diagnosis of: -
Answer✅✅DiGenorge syndrome -- microdeletion of 22q11, also called
velocardiofacial syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent), tubular
nose, wide spread eyes, etc
When verifying if a specimen is appropriate for cytogenetic analysis, what is the
MOST important data to accompany the specimen? - Answer✅✅Reason for referral
Which type of specimen should be requested for a cytogenetic analysis for turner
syndrome? - Answer✅✅Heparinized peripheral blood stored at room temperature
(NOT FROZEN)
, A blood sample can be used to study the cytogenetics of a malignancy only if: -
Answer✅✅Dividing leukemic cells are present in circulation
All of the cultures in a CO2 incubator failed, what aspects of the incubator are
important to monitor to assess the source of the problem? -
Answer✅✅Temperature,
CO2 level, and
Humidity level
The most important factor affecting the quality of chromosomes viewed under a
phase contrast scope is: - Answer✅✅Slide drying after the cells have been dropped
What is the modal number (default number of chromosomes in a cell) for a Down
syndrome translocation carrier? - Answer✅✅45
What is the best tissue to use for both a good success rate and abnormality
detection in spontaneous abortion miscarriages? - Answer✅✅Chorionic villi
What is a possible cause for a drying oven to not maintain a proper temperature? -
Answer✅✅The door does not seal properly
If a tech with a cold and sneezing does not take proper precautions while setting up
amniotic fluid samples, the specimen could become contaminated with: -
Answer✅✅Mycoplasma
--> the most common contaminant by a sick tech and can be detected through a
specific stain used on the sample
mycoplasma can induce abnormalities in a sample, so it is important to avoid
contamination
What about the metaphases on a slide are affected by slide making? -
Answer✅✅Cell density - thru dilution of the sample with fix
Banding - based on how dark/pale/refractive they are affects trypsin time
Chromosome spreading - affected by drying time
What test(s) should be ordered on a newborn with suspected Prader-Willi syndrome?
- Answer✅✅High resolution banding and FISH for the Prader-Willi locus on 15q11.2
When capturing a metaphase, a tech cannot get the met into focus, what is the
most likely cause? - Answer✅✅The camera is not parfocal (in focus with the
microscpe objectives) with the microscope
Exam| Verified Expert Exam
Questions with Clear Correct
Verified Answers| All Graded A+|
Latest Premium Update(2025-
2026)| 100% Guaranteed Success.
What is the best agent to use against mycoplasma? - Answer✅✅BM cyclin - a
combination of two antibiotics (tiamulin and minocycline) that effecticely targets
mycoplasma growth
For successful harvest, each new batch of hypotonic solution should be evaluated by:
- Answer✅✅comparing the new lot to a lot already in use
Colagenase, pronase, and trypsin are: - Answer✅✅Proteolytic enzymes - break
down tissues into single-cell suspensions
When harvesting peripheral blood cultures, asceptic technique no longer matters
following incubation with: - Answer✅✅Colcemid - mitotic spindle poison that halts
cells at metaphase
Samples that arrive at the cytogenetics lab should first be: - Answer✅✅Logged in to
the system
,Phytohemagglutinin (PHA) stimulates which type of cells into division? -
Answer✅✅T-cell lymphocytes
To avoid culture failure, monitoring reagents is crucial. When logging a culture
failure, what is the MOST important to document? - Answer✅✅Expiration dates of
reagents used
Which type of microscope is used when cleaning a CVS (chorionic villus) sample? -
Answer✅✅Dissection
Which cell type is most problematic if found in a CVS (chorionic villus) sample? -
Answer✅✅Decidua (maternal tissue)
When looking at a slide under a phase contrast scope and a lot of cytoplasm is still
seen around the mets, what is the most likely cause? - Answer✅✅innappropriate
slide drying time
What will help prevent microbial contamination and cross contamination of cultures?
- Answer✅✅Asceptic technique
When there are not enough mets to complete the study of a patient/case, what
needs to be done next? - Answer✅✅prepare additional slides from any remaining
sample
When looking at a dropped slide under a phase scope and mets are very spread out
and sparse, what could help fix the issue? - Answer✅✅Increading the drying time
by using warm slides
What is true of an inverted microscope? - Answer✅✅the objectives are beneath
the stage
High-resolution banding is indicated for a possible diagnosis of: -
Answer✅✅DiGenorge syndrome -- microdeletion of 22q11, also called
velocardiofacial syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent), tubular
nose, wide spread eyes, etc
When verifying if a specimen is appropriate for cytogenetic analysis, what is the
MOST important data to accompany the specimen? - Answer✅✅Reason for referral
Which type of specimen should be requested for a cytogenetic analysis for turner
syndrome? - Answer✅✅Heparinized peripheral blood stored at room temperature
(NOT FROZEN)
, A blood sample can be used to study the cytogenetics of a malignancy only if: -
Answer✅✅Dividing leukemic cells are present in circulation
All of the cultures in a CO2 incubator failed, what aspects of the incubator are
important to monitor to assess the source of the problem? -
Answer✅✅Temperature,
CO2 level, and
Humidity level
The most important factor affecting the quality of chromosomes viewed under a
phase contrast scope is: - Answer✅✅Slide drying after the cells have been dropped
What is the modal number (default number of chromosomes in a cell) for a Down
syndrome translocation carrier? - Answer✅✅45
What is the best tissue to use for both a good success rate and abnormality
detection in spontaneous abortion miscarriages? - Answer✅✅Chorionic villi
What is a possible cause for a drying oven to not maintain a proper temperature? -
Answer✅✅The door does not seal properly
If a tech with a cold and sneezing does not take proper precautions while setting up
amniotic fluid samples, the specimen could become contaminated with: -
Answer✅✅Mycoplasma
--> the most common contaminant by a sick tech and can be detected through a
specific stain used on the sample
mycoplasma can induce abnormalities in a sample, so it is important to avoid
contamination
What about the metaphases on a slide are affected by slide making? -
Answer✅✅Cell density - thru dilution of the sample with fix
Banding - based on how dark/pale/refractive they are affects trypsin time
Chromosome spreading - affected by drying time
What test(s) should be ordered on a newborn with suspected Prader-Willi syndrome?
- Answer✅✅High resolution banding and FISH for the Prader-Willi locus on 15q11.2
When capturing a metaphase, a tech cannot get the met into focus, what is the
most likely cause? - Answer✅✅The camera is not parfocal (in focus with the
microscpe objectives) with the microscope
