MBIO 3401 MIDTERM NEWEST 2025 ACTUAL EXAM COMPLETE
QUESTIONS AND CORRECT DETAILED ANSWERS (VERIFIED ANSWERS)
|ALREADY GRADED A+|BRAND NEW!!
What are the 4 traits of a microbe? -
Answer--Too small to be seen by human eye
-Grow and reproduce in nature
-Active metabolism
-Respond to stimuli
Diameter of a typical microbe? -
Answer-0.2 micrometers (μm) in diameter
Who was the first person to observe single-celled microbes? -
Answer-Antonie van Leeuwenhoek
Where do microbes come from? -
Answer-they have parents
who disproved theory of spontaneous generation? -
Answer-Louis Pasteur
Louis Pasteur contribution to microbiology? -
Answer-disproved spontaneous generation
Who proved the link between microbes and infectious disease? -
Answer-Robert Koch
Robert Koch's contribution to microbiology? -
Answer-Link between microbes and infectious disease.
What are the two primary types of cells? -
Answer-Prokaryotic and Eukaryotic
Is bacteria prokaryotic or eukaryotic? -
Answer-Prokaryotic
Is archaea prokaryotic or eukaryotic? -
Answer-Prokaryotic
Primary purpose of light microscope? -
Answer-Improve contrast and resolution
What is contrast? -
Answer-the difference in brightness between the light and dark areas of an image
,What is resolution of an image? -
Answer-Ability to distinguish between two objects.
5 types of light microscopy? -
Answer--Bright Field
-Phase contrast
-Differential Interference Contrast
-Dark-field
-Fluorescence
,Color of Gram + cells after gram stain? -
Answer-Purple
Color of gram - cells after gram stain -
Answer-Red/pink
What is confocal scanning laser microscopy? -
Answer-A computerized fluorescent microscope that uses a laser source to create a 3D
image
Pros and Cons of Bright-field microscopy: -
Answer-Pros: Simple and affordable Cons: need staining and difficult to see
small/colorless cells
Pros and Cons of dark field microscopy: -
Answer-Pros: Highly sensitive for small cells/structures
Cons: poor resolution and prone to artifacts
Pros and Cons of Phase contrast microscopy and differential interference
microscopy: -
Answer-Pros: See live cells w/out stain, see some internal structures
Cons: Hard for small cells
Pros and Cons of Fluorescence and Confocal Microscopes: -
Answer-Pros:
High resolution, 3D, vibrant colors, live cells
Cons: expensive
What is electron microscopy? -
Answer-Uses electrons rather than visual light
Advantage of electron microscopy? -
Answer-Greater resolution than light microscope
Role of transmission electron microscopy (TEM): -
Answer--See internal structures
-Obtain 3D images
Role of Scanning Electron Microscopes (SEM): -
Answer-see surface and external structures
What is passive transport? -
Answer-The movement of materials across the cell membrane without using cellular
energy and with concentration gradient
What is active transport? -
, Answer-themovement of ions or molecules across a cell membrane into a region of
higher concentration, assisted by enzymes and requiring energy.
Role of the cell wall? -
Answer-Maintain shape and rigidity
Parts of a phospholipid: -
Answer-Polar head and non polar tail
Where is peptidoglycan not found? -
Answer-Archaea and Eukaryotes
Where is peptidoglycan found? -
Answer-bacteria
QUESTIONS AND CORRECT DETAILED ANSWERS (VERIFIED ANSWERS)
|ALREADY GRADED A+|BRAND NEW!!
What are the 4 traits of a microbe? -
Answer--Too small to be seen by human eye
-Grow and reproduce in nature
-Active metabolism
-Respond to stimuli
Diameter of a typical microbe? -
Answer-0.2 micrometers (μm) in diameter
Who was the first person to observe single-celled microbes? -
Answer-Antonie van Leeuwenhoek
Where do microbes come from? -
Answer-they have parents
who disproved theory of spontaneous generation? -
Answer-Louis Pasteur
Louis Pasteur contribution to microbiology? -
Answer-disproved spontaneous generation
Who proved the link between microbes and infectious disease? -
Answer-Robert Koch
Robert Koch's contribution to microbiology? -
Answer-Link between microbes and infectious disease.
What are the two primary types of cells? -
Answer-Prokaryotic and Eukaryotic
Is bacteria prokaryotic or eukaryotic? -
Answer-Prokaryotic
Is archaea prokaryotic or eukaryotic? -
Answer-Prokaryotic
Primary purpose of light microscope? -
Answer-Improve contrast and resolution
What is contrast? -
Answer-the difference in brightness between the light and dark areas of an image
,What is resolution of an image? -
Answer-Ability to distinguish between two objects.
5 types of light microscopy? -
Answer--Bright Field
-Phase contrast
-Differential Interference Contrast
-Dark-field
-Fluorescence
,Color of Gram + cells after gram stain? -
Answer-Purple
Color of gram - cells after gram stain -
Answer-Red/pink
What is confocal scanning laser microscopy? -
Answer-A computerized fluorescent microscope that uses a laser source to create a 3D
image
Pros and Cons of Bright-field microscopy: -
Answer-Pros: Simple and affordable Cons: need staining and difficult to see
small/colorless cells
Pros and Cons of dark field microscopy: -
Answer-Pros: Highly sensitive for small cells/structures
Cons: poor resolution and prone to artifacts
Pros and Cons of Phase contrast microscopy and differential interference
microscopy: -
Answer-Pros: See live cells w/out stain, see some internal structures
Cons: Hard for small cells
Pros and Cons of Fluorescence and Confocal Microscopes: -
Answer-Pros:
High resolution, 3D, vibrant colors, live cells
Cons: expensive
What is electron microscopy? -
Answer-Uses electrons rather than visual light
Advantage of electron microscopy? -
Answer-Greater resolution than light microscope
Role of transmission electron microscopy (TEM): -
Answer--See internal structures
-Obtain 3D images
Role of Scanning Electron Microscopes (SEM): -
Answer-see surface and external structures
What is passive transport? -
Answer-The movement of materials across the cell membrane without using cellular
energy and with concentration gradient
What is active transport? -
, Answer-themovement of ions or molecules across a cell membrane into a region of
higher concentration, assisted by enzymes and requiring energy.
Role of the cell wall? -
Answer-Maintain shape and rigidity
Parts of a phospholipid: -
Answer-Polar head and non polar tail
Where is peptidoglycan not found? -
Answer-Archaea and Eukaryotes
Where is peptidoglycan found? -
Answer-bacteria
