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AMT MLS Exam Questions and
Answers A+ Graded (2025)
Which immunoassay methodology is the emission of light by molecules in an
excited state with a limited amount of heat as the result of a chemical reaction?
Question
a.Chemiluminescence
b.Immunochromatography
c.Photometry
d.Spectrophotometry - CORRECT ANSWER-a. Chemiluminescence
Chemiluminescence is the emission of light by molecules in an excited state with
a limited amount of heat (luminescence) as the result of a chemical reaction.
Chemiluminescent assays are ultrasensitive and are widely used in automated
immunoassays and DNA probe assay systems. Chemiluminescence has excellent
sensitivity and dynamic range. Chemiluminescent labels can be attached to an
antigen or an antibody. Most chemiluminescent reagents and conjugates are
stable and relatively nontoxic.
What characteristics/functions do calibrators have?
Question
a.Contains a known amount of analyte being tested
b.Monitors the quality of reagents
c.Monitors the quality of the sample
d.Prevents equipment failure - CORRECT ANSWER-a. Contains a known amount
of analyte being tested
Calibrators are used to establish a correlation between the measurement
response and the concentration or amount of the substance that is being
measured by the test procedure.
Absorbance can be calculated easily from percent transmission using which
calculation?
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a.2 - log10 %T
b.2 + log10 %T
c.log10 %T - 2
d.log10 %T + 2 - CORRECT ANSWER-a. 2 - log10 %T
Absorbance can be calculated easily from percent transmission using 2 - log10
%T. If all the light passes through a solution without any absorption, absorbance
is zero and the %T is 100%. If all the light is absorbed, %T is zero, and absorption
is infinite.
What is a substance or constituent in which the laboratory conducts testing?
a.A solution
b.An additive
c.An analyte
d.An anticoagulant - CORRECT ANSWER-c. An analyte
In the testing process, analytes or constituents are measured.
What does a common application of the polarized light microscope in the clinical
laboratory do?
Determine FANAs with fluorescence
b.Determine neutrophilic from lymphocytic leukemia
c.Examine histochemical reactions
d.Study crystals in urine and in cells - CORRECT ANSWER-d. study crystals in
urine and cells
What is the chelation of calcium in the EDTA tube?
a.Maintains a constant temperature for components
b.Prevents coagulation by inhibiting coagulation
c.Prevents the separation of blood components
d.Stops the lysing of the red blood cells - CORRECT ANSWER-b. Prevents
coagulation by inhibiting coagulation
CLSI recommends the salts of the chelating (calcium binding) agent EDTA as the
anticoagulant of choice for blood cell counting and sizing.
How must a laboratory operate to handle TB sputum and TM materials?
a.Biosafety level of 2+ or 3
b.Biosafety level 4
c.Location where air exchange is continuous
d.Location where fume hood exhausts are monitored daily - CORRECT ANSWER-
a. Biosafety level of 2+ or 3
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BSL-3 builds upon the requirements of BSL-2. Organisms that can cause serious
or potentially lethal disease through respiratory transmission are typically
worked with in a BSL-3 laboratory. Example: Mycobacterium tuberculosis.
What are Class A fire extinguishers used for?
a.Electric equipment
b.Flammable liquids
c.Ordinary combustibles
d.Powdered metal (combustible) material - CORRECT ANSWER-Ordinary
combustibles.
Class A fire extinguishers are classified as ordinary combustible. Additionally,
Class A fire extinguishers have a numerical rating based on UL-conducted tests
and designed to determine the extinguishing potential for each size and type of
extinguisher
Which part of the microscope directs and focuses the beam of light from the bulb
onto the material under examination?
a.Aperture
b.C- mount
c.Condenser
d.Diaphragm - CORRECT ANSWER-Condenser
The condenser is the substage lens of the microscope that focuses light on the
specimen slide
How many milliliters can a 3% solution make if 6 grams of solute are available?
a.100 mL
b.200 mL
c.300 mL
d.400 mL - CORRECT ANSWER-Correct Answer: 200 mL
3% of solution means 3 g of solute in 100 mL of solution.
1 g of solute contains = (100/3) mL
So, in 6 g of solute = (100/3) x 6 =200 mL.
200 mL of 3% solution is needed for 6 g of solute.
When seven points in a row in an upward or downward direction are seen on a
quality control graph it is called a
a.deviation.
b.shift.
c.trend.
d.variance. - CORRECT ANSWER-trend.
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A trend is seven points in a row in an upward direction or seven points in a row in
a downward direction. With a trend, it doesn't matter if the centerline is crossed
What category of bioterrorism agents include pathogens that are moderately easy
to disseminate, result in moderate and low morbidity rates, and require specific
enhancements of the CDC's diagnostic capacity and enhanced disease
surveillance?
Category A
b.Category B
c.Category C
d.Category D - CORRECT ANSWER-Category B
Bioterrorism agents are a concern to laboratories. These agents are divided into
categories A, B and C; and Category B agents have the second highest priority.
Category B agents have moderate morbidity (suffering from the disease) with low
mortality (death) rates. Ricin toxin is an example of Category B agent.
What category of bioterrorism agents have the highest priority and pose a risk to
national security because they can be easily disseminated or transmitted from
person to person, result in high mortality rates and have the potential for major
public health impact?
a.Category A
b.Category B
c.Category C
d.Category D - CORRECT ANSWER-orrect Answer: Category A
Bioterrorism agents are a concern to laboratories. These agents are divided into
categories A, B and C. Category A agents have the highest priority. Anthrax and
Smallpox are examples of Category A agents
What are two general sources of blood for clinical laboratory tests?
a. In vitro and in vivo blood
b.Peripheral (or capillary) blood and venous blood
c.Serum and plasma
d.Venous blood and in vivo blood - CORRECT ANSWER-Correct Answer:
Peripheral (or capillary) blood and venous blood
The Clinical and Laboratory Standards Institute (CLSI), formerly the National
Committee for Clinical Laboratory Standards (NCCLS), has set standards for the
collection of venous blood (venipuncture, or phlebotomy) and capillary blood
(skin puncture).
What category are agents anthrax, botulism, plague, smallpox, tularemia,
filoviruses, and arenaviruses classified as?
a.Category A
b.Category B
AMT MLS Exam Questions and
Answers A+ Graded (2025)
Which immunoassay methodology is the emission of light by molecules in an
excited state with a limited amount of heat as the result of a chemical reaction?
Question
a.Chemiluminescence
b.Immunochromatography
c.Photometry
d.Spectrophotometry - CORRECT ANSWER-a. Chemiluminescence
Chemiluminescence is the emission of light by molecules in an excited state with
a limited amount of heat (luminescence) as the result of a chemical reaction.
Chemiluminescent assays are ultrasensitive and are widely used in automated
immunoassays and DNA probe assay systems. Chemiluminescence has excellent
sensitivity and dynamic range. Chemiluminescent labels can be attached to an
antigen or an antibody. Most chemiluminescent reagents and conjugates are
stable and relatively nontoxic.
What characteristics/functions do calibrators have?
Question
a.Contains a known amount of analyte being tested
b.Monitors the quality of reagents
c.Monitors the quality of the sample
d.Prevents equipment failure - CORRECT ANSWER-a. Contains a known amount
of analyte being tested
Calibrators are used to establish a correlation between the measurement
response and the concentration or amount of the substance that is being
measured by the test procedure.
Absorbance can be calculated easily from percent transmission using which
calculation?
,mailto:://www.stuvia.com/user/BRAINSCAPE1
a.2 - log10 %T
b.2 + log10 %T
c.log10 %T - 2
d.log10 %T + 2 - CORRECT ANSWER-a. 2 - log10 %T
Absorbance can be calculated easily from percent transmission using 2 - log10
%T. If all the light passes through a solution without any absorption, absorbance
is zero and the %T is 100%. If all the light is absorbed, %T is zero, and absorption
is infinite.
What is a substance or constituent in which the laboratory conducts testing?
a.A solution
b.An additive
c.An analyte
d.An anticoagulant - CORRECT ANSWER-c. An analyte
In the testing process, analytes or constituents are measured.
What does a common application of the polarized light microscope in the clinical
laboratory do?
Determine FANAs with fluorescence
b.Determine neutrophilic from lymphocytic leukemia
c.Examine histochemical reactions
d.Study crystals in urine and in cells - CORRECT ANSWER-d. study crystals in
urine and cells
What is the chelation of calcium in the EDTA tube?
a.Maintains a constant temperature for components
b.Prevents coagulation by inhibiting coagulation
c.Prevents the separation of blood components
d.Stops the lysing of the red blood cells - CORRECT ANSWER-b. Prevents
coagulation by inhibiting coagulation
CLSI recommends the salts of the chelating (calcium binding) agent EDTA as the
anticoagulant of choice for blood cell counting and sizing.
How must a laboratory operate to handle TB sputum and TM materials?
a.Biosafety level of 2+ or 3
b.Biosafety level 4
c.Location where air exchange is continuous
d.Location where fume hood exhausts are monitored daily - CORRECT ANSWER-
a. Biosafety level of 2+ or 3
,mailto:://www.stuvia.com/user/BRAINSCAPE1
BSL-3 builds upon the requirements of BSL-2. Organisms that can cause serious
or potentially lethal disease through respiratory transmission are typically
worked with in a BSL-3 laboratory. Example: Mycobacterium tuberculosis.
What are Class A fire extinguishers used for?
a.Electric equipment
b.Flammable liquids
c.Ordinary combustibles
d.Powdered metal (combustible) material - CORRECT ANSWER-Ordinary
combustibles.
Class A fire extinguishers are classified as ordinary combustible. Additionally,
Class A fire extinguishers have a numerical rating based on UL-conducted tests
and designed to determine the extinguishing potential for each size and type of
extinguisher
Which part of the microscope directs and focuses the beam of light from the bulb
onto the material under examination?
a.Aperture
b.C- mount
c.Condenser
d.Diaphragm - CORRECT ANSWER-Condenser
The condenser is the substage lens of the microscope that focuses light on the
specimen slide
How many milliliters can a 3% solution make if 6 grams of solute are available?
a.100 mL
b.200 mL
c.300 mL
d.400 mL - CORRECT ANSWER-Correct Answer: 200 mL
3% of solution means 3 g of solute in 100 mL of solution.
1 g of solute contains = (100/3) mL
So, in 6 g of solute = (100/3) x 6 =200 mL.
200 mL of 3% solution is needed for 6 g of solute.
When seven points in a row in an upward or downward direction are seen on a
quality control graph it is called a
a.deviation.
b.shift.
c.trend.
d.variance. - CORRECT ANSWER-trend.
, mailto:://www.stuvia.com/user/BRAINSCAPE1
A trend is seven points in a row in an upward direction or seven points in a row in
a downward direction. With a trend, it doesn't matter if the centerline is crossed
What category of bioterrorism agents include pathogens that are moderately easy
to disseminate, result in moderate and low morbidity rates, and require specific
enhancements of the CDC's diagnostic capacity and enhanced disease
surveillance?
Category A
b.Category B
c.Category C
d.Category D - CORRECT ANSWER-Category B
Bioterrorism agents are a concern to laboratories. These agents are divided into
categories A, B and C; and Category B agents have the second highest priority.
Category B agents have moderate morbidity (suffering from the disease) with low
mortality (death) rates. Ricin toxin is an example of Category B agent.
What category of bioterrorism agents have the highest priority and pose a risk to
national security because they can be easily disseminated or transmitted from
person to person, result in high mortality rates and have the potential for major
public health impact?
a.Category A
b.Category B
c.Category C
d.Category D - CORRECT ANSWER-orrect Answer: Category A
Bioterrorism agents are a concern to laboratories. These agents are divided into
categories A, B and C. Category A agents have the highest priority. Anthrax and
Smallpox are examples of Category A agents
What are two general sources of blood for clinical laboratory tests?
a. In vitro and in vivo blood
b.Peripheral (or capillary) blood and venous blood
c.Serum and plasma
d.Venous blood and in vivo blood - CORRECT ANSWER-Correct Answer:
Peripheral (or capillary) blood and venous blood
The Clinical and Laboratory Standards Institute (CLSI), formerly the National
Committee for Clinical Laboratory Standards (NCCLS), has set standards for the
collection of venous blood (venipuncture, or phlebotomy) and capillary blood
(skin puncture).
What category are agents anthrax, botulism, plague, smallpox, tularemia,
filoviruses, and arenaviruses classified as?
a.Category A
b.Category B
