BIO 320 EXAM 2
(LECTURES 10-18)
proteins sorted to each organelle how? - CORRECT REPLY>>>>>>-
specialized membrane
ribosomes in cytosol
-translate proteins
-exception: some mt proteins
transport across membrane of whatever organelle
-signal in protein
[sequence of aa]
-receptor for that signal
-receptor brings protein to correct membrane
-translocation machinery
-energy for transfer
what if no targeting signals?
-remain in cytosol
,sorting signals - CORRECT REPLY>>>>>>-common between all
sorting signals
-cannot be deleted
-cannot be changed
-only part required for sorting
-reacts with receptor
-some = removed after arrival
-some = stay even after arrival
for the ER
-into: at N term
-usually co trans
-bring it back to: at C term
for the nucleus
-in the middle of the protein
-signals on the SURFACE of protein
-only one using gated transport
-basic NLS = 5 basic aa = ARG, LYS, HIS
-needs ALL 5 to be basic
for the MT
,-usually post trans
mechanisms of protein transport - CORRECT REPLY>>>>>>-
transmembrane
-across bilayer
-either co or post translational
vesicular transport
-uses vesicle budding
-er, golgi, lysosomes
gated transport
-across a selective gate
-nuclear pore
nucleus origin - CORRECT REPLY>>>>>>-explains relationship
between
-nucleus
-secretory membranes
1. DNA enclosed in invagination of PM
2. invagination = 2 membranes
, 3. invagination connect to PM w/ secretory pathway
4. break connection with PM = current nuclear env = connected to ER
membranes
processes in nucleus
-transcription
-dna replication
-ribosome assembly
what must happen
-hella proteins come in
-hella proteins come out
-nuclear proteins synth in cytosol, must enter
-ribosomal subunits, RNAs, etc must exit
nucleus structure - CORRECT REPLY>>>>>>-2 membranes
-inner
-outer
-perinuclear space = in between, continuous w/ ER lumen
nuclear pore
(LECTURES 10-18)
proteins sorted to each organelle how? - CORRECT REPLY>>>>>>-
specialized membrane
ribosomes in cytosol
-translate proteins
-exception: some mt proteins
transport across membrane of whatever organelle
-signal in protein
[sequence of aa]
-receptor for that signal
-receptor brings protein to correct membrane
-translocation machinery
-energy for transfer
what if no targeting signals?
-remain in cytosol
,sorting signals - CORRECT REPLY>>>>>>-common between all
sorting signals
-cannot be deleted
-cannot be changed
-only part required for sorting
-reacts with receptor
-some = removed after arrival
-some = stay even after arrival
for the ER
-into: at N term
-usually co trans
-bring it back to: at C term
for the nucleus
-in the middle of the protein
-signals on the SURFACE of protein
-only one using gated transport
-basic NLS = 5 basic aa = ARG, LYS, HIS
-needs ALL 5 to be basic
for the MT
,-usually post trans
mechanisms of protein transport - CORRECT REPLY>>>>>>-
transmembrane
-across bilayer
-either co or post translational
vesicular transport
-uses vesicle budding
-er, golgi, lysosomes
gated transport
-across a selective gate
-nuclear pore
nucleus origin - CORRECT REPLY>>>>>>-explains relationship
between
-nucleus
-secretory membranes
1. DNA enclosed in invagination of PM
2. invagination = 2 membranes
, 3. invagination connect to PM w/ secretory pathway
4. break connection with PM = current nuclear env = connected to ER
membranes
processes in nucleus
-transcription
-dna replication
-ribosome assembly
what must happen
-hella proteins come in
-hella proteins come out
-nuclear proteins synth in cytosol, must enter
-ribosomal subunits, RNAs, etc must exit
nucleus structure - CORRECT REPLY>>>>>>-2 membranes
-inner
-outer
-perinuclear space = in between, continuous w/ ER lumen
nuclear pore
