BIOD 171/MICROBIOLOGY LAB 7 PORTAGE
LEARNING EXAM 5 NEWEST (2025 VERSION)
FREQUENTLY TESTED QUESTIONS WITH
Page | 1
CORRECT DETAILED AND VERIFIED ANSWERS|
GURANTEED PASS| TOP RATED A+.
BIOD 171/MICROBIOLOGY
Pass the BIOD 171: Microbiology exam 2025/2026 with confidence. This
exam resource features questions in areas like: microorganisms and their
impact on human health, infection, and disease prevention. It is commonly
taken by nursing, pre-med, and health science students.
Define the concept of universal precautions. .... ANSWER
......Universal precautions mean any and all samples, whether known
or unknown, are to be treated as potentially hazardous (or pathogenic)
materials.
What would a researcher be certain to note while analyzing a microbial
sample? .... ANSWER ......1. Size and shape
2. Any observable motility
3. Gram status (positive or negative)
4. The presence of any chemical reactions
5. Changes in color localized to the organism or the surrounding media
6. Capture (or draw) images of any of the characteristics described
above
, Which non-selective yet differential agar plate should be used if a
Streptococcus sample is suspected? .... ANSWER ......A BAP (blood
agar plate) would be the quickest way to identify and classify a
Page | 2 potential streptococcus microbe based on its hemolytic properties.
What disease can result if a patient with streptococcal pharyngitis goes
untreated? .... ANSWER ......Rheumatic fever occurs in approximately
3% of untreated cases of streptococcal pharyngitis, presenting
approximately 2-3 weeks post-infection.
Streptococcus, E. coli, pseudomonas, and klebsiella are all capable of
causing what disease? .... ANSWER ......All of the above foreign
microbes are capable of causing septicemia, also known as blood
poisoning.
What is a facultative anaerobe? .... ANSWER ......A facultative
anaerobe is capable of growth under both aerobic (with oxygen) and
anaerobic (in the absence of oxygen) condition.
How can Staphylococcus be differentially tested? .... ANSWER
......Staphylococcus species are capable of growth in the presence of
bile salts.
A bacterial swab is taken from a patient with impetigo and streaked
across an MSA plate. Following incubation at 37C, what will be the color
of the agar? .... ANSWER ......The causative agent of impetigo is
staph aureus, a Gram-positive bacterium capable of fermenting
, mannitol. Fermentation will lower the pH of the agar and change the
dye color from red to yellow.
Page | 3
If a patient were suspected to have tuberculosis (TB), which differential
stain would you select and why? .... ANSWER ......To stain for TB,
one would utilize an acid-fast stain to best visual the foreign microbe.
As a mycobacterium tuberculosis would stain poorly (indeterminate)
using the Gram stain.
According to the module, which mycobacterium infection will be treated
free of charge by the World Health Organization (WHO)? .... ANSWER
......Leprosy is completely curable using a multiple-drug therapy
regimen consisting of various combinations of the drugs dapsone,
rifampin and clofazimine—all of which is given free of charge.
What are the three main conditions caused by the botulism toxin? ....
ANSWER ......Foodborne, infant and wound. Foodborne botulism is
often the result of improperly canned, contaminated, low-acidic foods
such as green beans, corn and beats. Infant botulism occurs when
children, usually under the age of 6 months consume foods containing
the bacteria. A potential source of infant botulism is honey (which is
why pediatricians recommend avoiding feeding honey to infants) but
can also be found in common places such as soil. Wound botulism
occurs when C. botulinum bacteria colonize a deep wound, often a
puncture such as those who inject street drugs such as heroine. As
contaminated needles are injected through the skin, spores enter the
body and, in the absence of oxygen, release the toxin.
, Why are infants (< 6 months) more susceptible to botulism? ....
ANSWER ......Due to infants still maturing intestinal tract and lack of
an adult microbiota, the bacteria are able to colonize and produce the
Page | 4 associated toxin. In adults, only the direct ingestion of the pre-formed
toxin causes disease—ingestion of C. botulinum or the associated
spores do not cause illness as a mature intestinal microbiota
sufficiently prevents bacterial germination and growth.
What is the mechanism of action of the toxin produced by clostridium
tentani (tetanus)? .... ANSWER ......The toxin acts on inhibitory
neurons causing systemic muscle stiffness and spasms. Muscle stiffness
can be observed in the jaw (lock-jaw) or spread systemically (if left
untreated) to muscles relating to respiratory function.
What Gram-negative bacterium might you come into contact with in a
unsanitary hot tub? .... ANSWER ......Legionella is often associated
with unclean water systems such as hot tubs, hot water tanks and AC
units. Notably, legionella can only be transmitted through water
droplets small enough to be inhaled.
What are the three forms of plague, and what regions do they affect? ....
ANSWER ......Bubonic (lymph), pneumonic (lungs) and septicemic
(blood). Bubonic plague is best characterized by painfully swollen
lymph nodes. Pneumonic plague affects the respiratory system (lungs).
The most rare of all three plagues, septicemic plague targets the blood
system and, if left untreated, will develop into a life-threatening illness
as the blood-borne bacteria can infect all major organs.
LEARNING EXAM 5 NEWEST (2025 VERSION)
FREQUENTLY TESTED QUESTIONS WITH
Page | 1
CORRECT DETAILED AND VERIFIED ANSWERS|
GURANTEED PASS| TOP RATED A+.
BIOD 171/MICROBIOLOGY
Pass the BIOD 171: Microbiology exam 2025/2026 with confidence. This
exam resource features questions in areas like: microorganisms and their
impact on human health, infection, and disease prevention. It is commonly
taken by nursing, pre-med, and health science students.
Define the concept of universal precautions. .... ANSWER
......Universal precautions mean any and all samples, whether known
or unknown, are to be treated as potentially hazardous (or pathogenic)
materials.
What would a researcher be certain to note while analyzing a microbial
sample? .... ANSWER ......1. Size and shape
2. Any observable motility
3. Gram status (positive or negative)
4. The presence of any chemical reactions
5. Changes in color localized to the organism or the surrounding media
6. Capture (or draw) images of any of the characteristics described
above
, Which non-selective yet differential agar plate should be used if a
Streptococcus sample is suspected? .... ANSWER ......A BAP (blood
agar plate) would be the quickest way to identify and classify a
Page | 2 potential streptococcus microbe based on its hemolytic properties.
What disease can result if a patient with streptococcal pharyngitis goes
untreated? .... ANSWER ......Rheumatic fever occurs in approximately
3% of untreated cases of streptococcal pharyngitis, presenting
approximately 2-3 weeks post-infection.
Streptococcus, E. coli, pseudomonas, and klebsiella are all capable of
causing what disease? .... ANSWER ......All of the above foreign
microbes are capable of causing septicemia, also known as blood
poisoning.
What is a facultative anaerobe? .... ANSWER ......A facultative
anaerobe is capable of growth under both aerobic (with oxygen) and
anaerobic (in the absence of oxygen) condition.
How can Staphylococcus be differentially tested? .... ANSWER
......Staphylococcus species are capable of growth in the presence of
bile salts.
A bacterial swab is taken from a patient with impetigo and streaked
across an MSA plate. Following incubation at 37C, what will be the color
of the agar? .... ANSWER ......The causative agent of impetigo is
staph aureus, a Gram-positive bacterium capable of fermenting
, mannitol. Fermentation will lower the pH of the agar and change the
dye color from red to yellow.
Page | 3
If a patient were suspected to have tuberculosis (TB), which differential
stain would you select and why? .... ANSWER ......To stain for TB,
one would utilize an acid-fast stain to best visual the foreign microbe.
As a mycobacterium tuberculosis would stain poorly (indeterminate)
using the Gram stain.
According to the module, which mycobacterium infection will be treated
free of charge by the World Health Organization (WHO)? .... ANSWER
......Leprosy is completely curable using a multiple-drug therapy
regimen consisting of various combinations of the drugs dapsone,
rifampin and clofazimine—all of which is given free of charge.
What are the three main conditions caused by the botulism toxin? ....
ANSWER ......Foodborne, infant and wound. Foodborne botulism is
often the result of improperly canned, contaminated, low-acidic foods
such as green beans, corn and beats. Infant botulism occurs when
children, usually under the age of 6 months consume foods containing
the bacteria. A potential source of infant botulism is honey (which is
why pediatricians recommend avoiding feeding honey to infants) but
can also be found in common places such as soil. Wound botulism
occurs when C. botulinum bacteria colonize a deep wound, often a
puncture such as those who inject street drugs such as heroine. As
contaminated needles are injected through the skin, spores enter the
body and, in the absence of oxygen, release the toxin.
, Why are infants (< 6 months) more susceptible to botulism? ....
ANSWER ......Due to infants still maturing intestinal tract and lack of
an adult microbiota, the bacteria are able to colonize and produce the
Page | 4 associated toxin. In adults, only the direct ingestion of the pre-formed
toxin causes disease—ingestion of C. botulinum or the associated
spores do not cause illness as a mature intestinal microbiota
sufficiently prevents bacterial germination and growth.
What is the mechanism of action of the toxin produced by clostridium
tentani (tetanus)? .... ANSWER ......The toxin acts on inhibitory
neurons causing systemic muscle stiffness and spasms. Muscle stiffness
can be observed in the jaw (lock-jaw) or spread systemically (if left
untreated) to muscles relating to respiratory function.
What Gram-negative bacterium might you come into contact with in a
unsanitary hot tub? .... ANSWER ......Legionella is often associated
with unclean water systems such as hot tubs, hot water tanks and AC
units. Notably, legionella can only be transmitted through water
droplets small enough to be inhaled.
What are the three forms of plague, and what regions do they affect? ....
ANSWER ......Bubonic (lymph), pneumonic (lungs) and septicemic
(blood). Bubonic plague is best characterized by painfully swollen
lymph nodes. Pneumonic plague affects the respiratory system (lungs).
The most rare of all three plagues, septicemic plague targets the blood
system and, if left untreated, will develop into a life-threatening illness
as the blood-borne bacteria can infect all major organs.
