AimToinvestigatetheeffectof a namedvariableonrate of enzyme controlled reaction
ampleEffectof PH Temperature Inhibitors
quipment
m beaker
Estate 57999ft yesssin thermometer
ethod
useforcepstoplacepieceoffilminto straw
usesyringeto measure4cm trypsininto a testtube
use anothersyringeto measure4cm of PHbufferplaceintest tube
placetest tube in a beak of water wait sminutesforcontentsto reach37 C
allowsenoughtime for liquid in test tube to reach 37 C
buffer used to controlpHbeingtested
place straw with film intosolution startstopwatch
when film is completely clear reaction hasfinished recordtime taken
repeatfordifferent pH values
repeatusing water instead oftrypsinasa colour
imitations
endpointissubjective humanerror timewasting
Temperatureof water bath decreased
rangeofpHbeingtestedfor was notwideenough
Amiffpgpare stainedsmashedcells set up anduse of anopticalmicroscope toseestagesof
Equipment
opticalmicroscope mi scalpel
coverslips witerspansides B teenager forceps 8EEp
Method
1 Heat HCl at 60 C in water bath
2 use scalpel to cut smallsampleof the root tip
3 Transferroot tip to HCl incubate for 5 minutes
breaks downcellulosecell wall so cell can beflattened
4 washsample in distilled water removeverytip with a scalpel
5 Place tip onmicroscopeslide add a fewdropsofstain
Tomake chromosomesvisible so cells undergoingmitosis can be seen
6 Lowercoverslip down ensure it doesn'tslip
preventsairbubbleswhichmaydistortimage
Ifcovership slides it coulddamagechromosomes
7 Place slide under amicroscope setobjectivelenson lowestmagnification
8 use coarseadjustment knobtomovelensdown above slide
e usefineadjustment knobto readjust focusuntilimageis clear
ampleEffectof PH Temperature Inhibitors
quipment
m beaker
Estate 57999ft yesssin thermometer
ethod
useforcepstoplacepieceoffilminto straw
usesyringeto measure4cm trypsininto a testtube
use anothersyringeto measure4cm of PHbufferplaceintest tube
placetest tube in a beak of water wait sminutesforcontentsto reach37 C
allowsenoughtime for liquid in test tube to reach 37 C
buffer used to controlpHbeingtested
place straw with film intosolution startstopwatch
when film is completely clear reaction hasfinished recordtime taken
repeatfordifferent pH values
repeatusing water instead oftrypsinasa colour
imitations
endpointissubjective humanerror timewasting
Temperatureof water bath decreased
rangeofpHbeingtestedfor was notwideenough
Amiffpgpare stainedsmashedcells set up anduse of anopticalmicroscope toseestagesof
Equipment
opticalmicroscope mi scalpel
coverslips witerspansides B teenager forceps 8EEp
Method
1 Heat HCl at 60 C in water bath
2 use scalpel to cut smallsampleof the root tip
3 Transferroot tip to HCl incubate for 5 minutes
breaks downcellulosecell wall so cell can beflattened
4 washsample in distilled water removeverytip with a scalpel
5 Place tip onmicroscopeslide add a fewdropsofstain
Tomake chromosomesvisible so cells undergoingmitosis can be seen
6 Lowercoverslip down ensure it doesn'tslip
preventsairbubbleswhichmaydistortimage
Ifcovership slides it coulddamagechromosomes
7 Place slide under amicroscope setobjectivelenson lowestmagnification
8 use coarseadjustment knobtomovelensdown above slide
e usefineadjustment knobto readjust focusuntilimageis clear
