1. Consider the following DNA sequence: 3'-TAC TGG GTT CCC-5'
a. Write out the complementary RNA sequence, including
indicating the 5' and 3'.
b. The poly-U's of strand termination are found on which end of the
RNA (5' or 3')?
c. Identify the start codon in the sequence you wrote in part a. of this
question.
a. 5'-AUG ACC CAA GGG-3'
b. 3'
c. AUG
2. Consider the following DNA sequence: 3'-AAC TAC TGT GTA -5'
a. Write out the complementary RNA sequence, including the 5' and 3'
b. Translation starts on which end of the RNA (5' or 3')?
c. Identify the start codon in the complementary sequence you wrote
in part a. of this question
a. 5'-UUG AUG ACA CAU -3'
b. 5'
c. AUG
3. True or False: Transcription of DNA into mRNA does not require an
RNA primer.
True
4. True or False: transcription of mRNA into DNA requires an
RNA primer
False
5. Why must the DNA within eukaryotes be complexed with
histone proteins, but not in bacteria? What happens to these
complexes during DNA replication?
-Histones are released in translation after transcription is complete.
The DNA binds to the histones to make it more condensed and then
leaves after the DNA transfer. In bacteria everything is relatively
smaller compared to Eukaryotes.
-There is a huge amount of DNA within a eukaryotic cell, and it needs
to be stored within a tiny space within the nucleus. Therefore, it is
complexed with proteins into chromatin that help to condense it into
an extremely compact form. When it is time for DNA replication to
occur, the complexes relax due to the proteins dissociating from it.
Then as soon as replication is complete at a certain point, the DNA
winds around the histone proteins and recondenses.
, 6. Match the following terms to the correct description: Ribosomes,
Codon, reverse transcription, shelterin complex, exon.
a. Write out the complementary RNA sequence, including
indicating the 5' and 3'.
b. The poly-U's of strand termination are found on which end of the
RNA (5' or 3')?
c. Identify the start codon in the sequence you wrote in part a. of this
question.
a. 5'-AUG ACC CAA GGG-3'
b. 3'
c. AUG
2. Consider the following DNA sequence: 3'-AAC TAC TGT GTA -5'
a. Write out the complementary RNA sequence, including the 5' and 3'
b. Translation starts on which end of the RNA (5' or 3')?
c. Identify the start codon in the complementary sequence you wrote
in part a. of this question
a. 5'-UUG AUG ACA CAU -3'
b. 5'
c. AUG
3. True or False: Transcription of DNA into mRNA does not require an
RNA primer.
True
4. True or False: transcription of mRNA into DNA requires an
RNA primer
False
5. Why must the DNA within eukaryotes be complexed with
histone proteins, but not in bacteria? What happens to these
complexes during DNA replication?
-Histones are released in translation after transcription is complete.
The DNA binds to the histones to make it more condensed and then
leaves after the DNA transfer. In bacteria everything is relatively
smaller compared to Eukaryotes.
-There is a huge amount of DNA within a eukaryotic cell, and it needs
to be stored within a tiny space within the nucleus. Therefore, it is
complexed with proteins into chromatin that help to condense it into
an extremely compact form. When it is time for DNA replication to
occur, the complexes relax due to the proteins dissociating from it.
Then as soon as replication is complete at a certain point, the DNA
winds around the histone proteins and recondenses.
, 6. Match the following terms to the correct description: Ribosomes,
Codon, reverse transcription, shelterin complex, exon.
