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BIOD 210 Exam 4 Portage learning SUMMER 2025

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1. Explain how have multidrug-resistant pathogens in agriculture been propagated. Livestock and animals are provided food treated with continuous low- dose antibiotics, which are used to enhance the growth of the animals. Over time, bacteria accrue mutations that lead to resistance to these antibiotics and eventually pathogenic bacteria are borne with the same resistance, and standard antibiotic treatment no longer works. 2. Explain how have multidrug resistant pathogens in health clinics been propagated For many years, the first course of treatment for illness was antibiotics. Health providers would use these drugs regardless of being able to diagnose illnesses as bacterial, viral, or something altogether different. Over time, the overuse of antibiotics led to bacteria that accrued mutations leading to resistance. Eventually more pathogenic bacteria arose with the same resistance, and standard antibiotic treatment no longer worked 3. When the bacteria are cultured but separated by a filter so that they cannot directly touch, one of the bacterial strains can grow well on minimal medium. What type of genetic transmission occurred in this experiment? Explain how you know. Transduction occurred in this experiment because the bacteria did not require direct contact to transmit the genetic material, like it would’ve happened with conjugation.

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Institution
BIOD 210
Course
BIOD 210

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1. Explain how have multidrug-resistant pathogens in agriculture been
propagated.
Livestock and animals are provided food treated with continuous low-
dose antibiotics, which are used to enhance the growth of the animals.
Over time, bacteria accrue mutations that lead to resistance to these
antibiotics and eventually pathogenic bacteria are borne with the
same resistance, and standard antibiotic treatment no longer works.

2. Explain how have multidrug resistant pathogens in health clinics been
propagated
For many years, the first course of treatment for illness was
antibiotics. Health providers would use these drugs regardless of
being able to diagnose illnesses as bacterial, viral, or something
altogether different. Over time, the overuse of antibiotics led to
bacteria that accrued mutations leading to resistance. Eventually
more pathogenic bacteria arose with the same resistance, and
standard antibiotic treatment no longer worked

3. When the bacteria are cultured but separated by a filter so that they
cannot directly touch, one of the bacterial strains can grow well on
minimal medium. What type of genetic transmission occurred in
this experiment? Explain how you know.
Transduction occurred in this experiment because the bacteria did not
require direct contact to transmit the genetic material, like it
would’ve happened with conjugation.

4. Two auxotrophic bacterial strains are propagated in the laboratory,
one deficient for leucine and the other deficient for biotin. When
cultured alone, they cannot grow on minimal medium, and when
combined in the same culture media, they still cannot grow in the
minimal media. Can you tell if genetic transmission occurred in this
experiment in the case of genes that control synthesis of biotin or
leucine?
It does not appear that genetic transmission has occurred in this
experiment involving these genes because even with direct contact,
there is no growth rescue for either of these auxotrophs.

5. Does spontaneous mutation positively or negatively affect evolution?
Briefly explain your answer.

, Spontaneous mutations positively affect evolution because the
mutations that make the organism better able to function or tolerate

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