Bio 325
BIO 325 Exam 2 Study Guide Questions
With 100% Correct Answers 2025
replicate
store information
express information
allow variation by mutation -Correct Answers ✔-to serve as genetic material, a
molecule must be able to __ (4)
Proteins and nucleic acids were the major candidates for the genetic material. -
Correct Answers ✔-before 1944, observations favored that ___
Griffith -Correct Answers ✔-____ found Evidence Favoring DNA as the Genetic
Material and Was First Obtained during the Study of Bacteria and Bacteriophages
avirulent to virulent -Correct Answers ✔-Griffith showed that Diplococcus
pneumoniae could be transformed from ______ to _____ strains
death -Correct Answers ✔-Living III S (virulent) + mouse = ?
Mouse lives -Correct Answers ✔-Living II R (avirulent) + mouse = ?
Mouse lives -Correct Answers ✔-Heath killed III S + mouse = ?
Mouse died, but Living III S recovered -Correct Answers ✔-Living II R + Heat
Killed III S + Mouse = ? (2)
Avery, MacLeod, and McCarty -Correct Answers ✔-___ demonstrated that the
transforming principle was DNA and not protein
Hershey and Chase -Correct Answers ✔-___ demonstrated that DNA enters the
bacterial cell during bacteriophage infection and directs viral production
Bio 325
,Bio 325
Transfection -Correct Answers ✔-proved conclusively that the viral DNA alone
contains all the necessary information for production of mature viruses; process by
which nucleic acids are introduced into mammalian cells
DNA is found only where the primary genetic function occurs.
Whereas protein is found throughout the cell. -Correct Answers ✔-indirect
evidence in support of DNA being the genetic material
UV Light -Correct Answers ✔-_____ capable of inducing mutations in the genetic
material(DNA and RNA) and is most mutagenic at a wavelength of 260 nm;
another reason that provides indirect evidence for DNA as the genetic material.
proteins -Correct Answers ✔-_____ absorb UV light most strongly at a wavelength
at which no significant mutagenic effects are observed.(280nm)
recombinant DNA technology -Correct Answers ✔-strongest direct evidence for
DNA as the genetic material comes from _____
recombinant DNA technology -Correct Answers ✔-Segments of eukaryotic DNA
corresponding to specific genes are isolated and spliced into the bacterial DNA;
used to isolate, replicate, and analyze genes.
RNA replicase -Correct Answers ✔-a polymerase that promotes synthesis of a
particular RNA in the presence of a template of RNA
dependent DNA polymerase reverse transcriptase -Correct Answers ✔-RNA serves
as a template for synthesis of a complementary DNA; DNA can be incorporated
into the host cell genome.
nucleotides -Correct Answers ✔-building blocks of DNA
a nitrogenous base
Bio 325
, Bio 325
a pentose sugar
a phosphate group -Correct Answers ✔-3 parts of nucleotides
adenine (A) and guanine (G). -Correct Answers ✔-purines
cytosine (C), thymine (T), and uracil (U) -Correct Answers ✔-pyrimidines
nucleoside -Correct Answers ✔-____ contains the nitrogenous base and the
pentose sugar.
nucleotide -Correct Answers ✔-____ contains the nitrogenous base and the pentose
sugar and the phosphate group
C-5' -Correct Answers ✔-location of the phosphate group on a nucleotide
phosphodiester bond -Correct Answers ✔-Nucleotides are linked by a ____ ____
between the phosphate group at the C-5' position and the OH group on the C-3'
position.
dTDP -Correct Answers ✔-...
Chargaff -Correct Answers ✔-_____ showed that the amount of A is proportional
to T and the amount of C is proportional to G, but the percentage of C + G does not
necessarily equal the percentage of A + T
X-ray diffraction -Correct Answers ✔-detected a periodicity of
3.4 angstroms (3.4-Å) repetitions* within the structure of the molecule, which
suggested to him that the bases were stacked like coins on top of one another
10 -Correct Answers ✔-how many base pairs per turn
complementarity -Correct Answers ✔-Chemical affinity between nitrogenous
bases of nucleic acid strands as a result of hydrogen bonding.
Bio 325
BIO 325 Exam 2 Study Guide Questions
With 100% Correct Answers 2025
replicate
store information
express information
allow variation by mutation -Correct Answers ✔-to serve as genetic material, a
molecule must be able to __ (4)
Proteins and nucleic acids were the major candidates for the genetic material. -
Correct Answers ✔-before 1944, observations favored that ___
Griffith -Correct Answers ✔-____ found Evidence Favoring DNA as the Genetic
Material and Was First Obtained during the Study of Bacteria and Bacteriophages
avirulent to virulent -Correct Answers ✔-Griffith showed that Diplococcus
pneumoniae could be transformed from ______ to _____ strains
death -Correct Answers ✔-Living III S (virulent) + mouse = ?
Mouse lives -Correct Answers ✔-Living II R (avirulent) + mouse = ?
Mouse lives -Correct Answers ✔-Heath killed III S + mouse = ?
Mouse died, but Living III S recovered -Correct Answers ✔-Living II R + Heat
Killed III S + Mouse = ? (2)
Avery, MacLeod, and McCarty -Correct Answers ✔-___ demonstrated that the
transforming principle was DNA and not protein
Hershey and Chase -Correct Answers ✔-___ demonstrated that DNA enters the
bacterial cell during bacteriophage infection and directs viral production
Bio 325
,Bio 325
Transfection -Correct Answers ✔-proved conclusively that the viral DNA alone
contains all the necessary information for production of mature viruses; process by
which nucleic acids are introduced into mammalian cells
DNA is found only where the primary genetic function occurs.
Whereas protein is found throughout the cell. -Correct Answers ✔-indirect
evidence in support of DNA being the genetic material
UV Light -Correct Answers ✔-_____ capable of inducing mutations in the genetic
material(DNA and RNA) and is most mutagenic at a wavelength of 260 nm;
another reason that provides indirect evidence for DNA as the genetic material.
proteins -Correct Answers ✔-_____ absorb UV light most strongly at a wavelength
at which no significant mutagenic effects are observed.(280nm)
recombinant DNA technology -Correct Answers ✔-strongest direct evidence for
DNA as the genetic material comes from _____
recombinant DNA technology -Correct Answers ✔-Segments of eukaryotic DNA
corresponding to specific genes are isolated and spliced into the bacterial DNA;
used to isolate, replicate, and analyze genes.
RNA replicase -Correct Answers ✔-a polymerase that promotes synthesis of a
particular RNA in the presence of a template of RNA
dependent DNA polymerase reverse transcriptase -Correct Answers ✔-RNA serves
as a template for synthesis of a complementary DNA; DNA can be incorporated
into the host cell genome.
nucleotides -Correct Answers ✔-building blocks of DNA
a nitrogenous base
Bio 325
, Bio 325
a pentose sugar
a phosphate group -Correct Answers ✔-3 parts of nucleotides
adenine (A) and guanine (G). -Correct Answers ✔-purines
cytosine (C), thymine (T), and uracil (U) -Correct Answers ✔-pyrimidines
nucleoside -Correct Answers ✔-____ contains the nitrogenous base and the
pentose sugar.
nucleotide -Correct Answers ✔-____ contains the nitrogenous base and the pentose
sugar and the phosphate group
C-5' -Correct Answers ✔-location of the phosphate group on a nucleotide
phosphodiester bond -Correct Answers ✔-Nucleotides are linked by a ____ ____
between the phosphate group at the C-5' position and the OH group on the C-3'
position.
dTDP -Correct Answers ✔-...
Chargaff -Correct Answers ✔-_____ showed that the amount of A is proportional
to T and the amount of C is proportional to G, but the percentage of C + G does not
necessarily equal the percentage of A + T
X-ray diffraction -Correct Answers ✔-detected a periodicity of
3.4 angstroms (3.4-Å) repetitions* within the structure of the molecule, which
suggested to him that the bases were stacked like coins on top of one another
10 -Correct Answers ✔-how many base pairs per turn
complementarity -Correct Answers ✔-Chemical affinity between nitrogenous
bases of nucleic acid strands as a result of hydrogen bonding.
Bio 325
