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THE PHLEBOTOMY MASTERY EXAM: EVERYTHING YOU
NEED TO KNOW! EXAM AND PRACTICE EXAM 2025/26 250
QUESTIONS
A drug known to interfere with urine tests should be discontinued 48-72 hours prior to the
test. A. True B. False - Correct Answer - A. True
Which WBC comprises 40%-60% of the white blood cells? A. Lymphocytes B. Neutrophils
C. Monocytes D. Basophils - Correct Answer - B. Neutrophils
How many hours before a blood test should a patient discontinue the use of medications
known to interfere with blood tests? A. 1-4 B. 4-24 C. 24-36 D. 48-72 - Correct Answer - B.
4-24
Which tube is drawn last in a standard venipuncture procedure? A. Blood culture tube B.
Lavender C. Light-blue D. Red - Correct Answer - B. Lavender
Where do blood cells originate? A. Heart B. Lungs C. Bone marrow D. Capillaries - Correct
Answer - C. Bone marrow
Which blood vessel is NOT part of systemic circulation? A. Brachial artery B. Cephalic vein
C. Pulmonary artery D. Vena cava - Correct Answer - C. Pulmonary artery
In the order of draw for capillary specimens should you draw tubes with or without
additives first? A. Without B. With - Correct Answer - B. With
What is a condition that could cause leukopenia? A. Chemotherapy B. Leukemia C. Low
blood sugar D. Coagulation - Correct Answer - B. Leukemia
An example of a nosocomial infection is A. Catheter site of ICU patient becomes infected
B. Healthcare worker contracts HBV from a needlestick C. Patient is admitted with chicken
pox D. Patient breaks out with measles one day after admission - Correct Answer - A.
Catheter site of ICU patient becomes infected
Lymph originates from A. Joint fluid B. Plasma C. Serum D. Tissue fluid - Correct Answer -
D. Tissue fluid
,2 of 34
Which vein in the antecubital fossa is usually easiest to palpate in an obese patient? A.
Median cubital B. Cephalic C. Basilic D. Ulnar - Correct Answer - B. Cephalic
What organ would a doctor be intested in if he ordered an ALP, AST, ALT, GTT and bilirubin?
A. Heart B. Liver C. Kidney D. Spleen - Correct Answer - B. Liver
Which fluid is obtained by lumbar puncture. A. Peritoneal B. Pleural C. Cerebrospinal D.
Synovial - Correct Answer - C. Cerebrospinal
How many times should a red top tube be inverted? A. 2 B. 5 C. 0 D. 8 - Correct Answer - C.
0
Capillary puncture reference ranges are higher for this analyte. A. Calcium B. Glucose C.
Phosphorus D. Protein - Correct Answer - B. Glucose
If someone is cyanotic their skin may appear ____________. A. Flushed B. Clammy C. Bluish
D. Yellow/orange - Correct Answer - C. Bluish
Which is NOT a common symptom of shock? A. Clammy skin B. Weak pulse C. Blinking
eyes D. Shallow breathing - Correct Answer - C. Blinking eyes
The OGTT test diagnoses ______________. A. High blood pressure B. Cholesterol C.
Diabetes D. Hemoglobin - Correct Answer - C. Diabetes
Serum is depleted of fibrogen. A. True B. False - Correct Answer - A. True
Latex sensitivity is an allergic reaction. A. True B. False - Correct Answer - A. True
There is a 9 to 1 ratio of blood to anticoagulant in a light blue tube. A. True B. False - Correct
Answer - A. True
Which is the best tube for collecting an ethanol specimen? A. EDTA B. Heparin C. Sodium
citrate D. Sodium fluoride - Correct Answer - D. Sodium fluoride
(gray top)
Glass particle in serum seperator tubes A. Deter coagulation B. Enhance clotting C. Inhibit
glycolysis D. Prevent hemolysis - Correct Answer - B. Enhance clotting
Which element prevents glycolysis? A. EDTA B. Heparin C. Sodium Fluoride D. Potassium
oxalate - Correct Answer - D. Potassium oxalate
,3 of 34
What kind of blood do arteries and arterioles carry? A. Deoxygenated B. Oxygenated -
Correct Answer - B. Oxygenated
Blood cultures are collected in a ___________ tube. A. Red B. Yellow C. Blue D. Green -
Correct Answer - B. Yellow
The Good Samaritan Law allows health care workers to provide medical care above and
beyond their normal field without being sued. A. True B. False - Correct Answer - B. False
Blood cultures are used to diagnose ________________. A. Diabetes B. FUO C. FAQ D. PT -
Correct Answer - B. FUO
What is the preferred antiseptic for venipuncture? A. Betadine B. Iodine C. Isopropyl
alcohol D. Jack Daniels - Correct Answer - C. Isopropyl alcohol
The following is a test of the respiratory system A. ABGs B. CSF C. ETOH D. UA - Correct
Answer - A. ABGs
What part of the infant is a PKU test performed on? A. Toe B. Finger C. Heel D. Forehead -
Correct Answer - C. Heel
The myocardium is the layer of the heart that is made of ___________-. A. Muscle B.
Cartilage C. Endometrial tissue - Correct Answer - A. Muscle
Which is not a PPE? A. Mask B. Goggles C. Room dividers D. Gloves - Correct Answer - C.
Room dividers
To check for trace elements a phebotomist would use a ______ tube. A. Light blue B. Royal
blue C. Tiger top D. Yellow - Correct Answer - B. Royal blue
The following were collected for a routine venipuncture. Which item is incorrect? A. Tubes
B. Iodine swab C. Touniquet D. Safety needles - Correct Answer - B. Iodine swab
What causes vacuum tubes to fill with blood? A. Arterial blood pressure B. Patient fist
pumping C. Tourniquet pressure D. Tube vacuum - Correct Answer - D. Tube vacuum
Which laboratory section performs blood pregnancy tests? A. Chemistry B. Hematology C.
Serology D. Blood bank - Correct Answer - A. Chemistry
What special handling does an ABG require? A. Warming B. Chilling C. Shaking D. Pre-
warmed tubes - Correct Answer - B. Chilling
, 4 of 34
Using a butterfly may be necessary if the vein collapses with the evacuated tube system. A.
True B. False - Correct Answer - A. True
Arteries have valves that prevent the blood from flowing back. A. True B. False - Correct
Answer - B. False
Lipemic bloodserum appears: A. Bluish B. Milky C. Purple D. Thick - Correct Answer - B.
Milky
Microorganism cultures are collected in _____________tubes. A. Light-blue B. Royal blue C.
Green D. Yellow - Correct Answer - D. Yellow
Which of the following specimens are least likely to require special handling? A. Bilirubin
and Folate B. Cholesterol and Uric Acid C. Gastrin and Lactic Acid D. Homocystine and
renin - Correct Answer - B. Cholesterol and Uric Acid
A pad used to protect the patient's clothing. A. Flux B. Chux C. Tourniquet D. Antiseptic -
Correct Answer - B. Chux
The two circulations of the heart are _________and ___________. A. Tricuspid and Bicuspid
B. Pulmonary and Systemic C. Right Ventricle and Left Ventricle D. Right Atrium and Left
Atrium - Correct Answer - B. Pulmonary and Systemic
What are the 3 components of a urinalysis? A. Physical, chemical, microscopic exams B.
Gravity, clarity and color exams C. Blood, protein and glucose exams D. Hear, see and
taste exam - Correct Answer - A. Physical, chemical, microscopic exams
When you explain the venipuncture procedure to the patient and they agree to the test it is
called _______. A. Getting the blood B. Informed consent C. Constant talking D. HIPAA -
Correct Answer - B. Informed consent
At what intervals should blood be clotted during a bleeding time test? A. 15 seconds B. 20
seconds C. 30 seconds D. 35 seconds - Correct Answer - C. 30 seconds
Contact, droplet and airborne are ____________. A. Modes of transmission B. Portals of exit
C. Portals of entry D. Agents - Correct Answer - A. Modes of transmission
What is the size (in inches) of the needle routinely used for venipuncture? A. 1-1.5 B. 2-3 C.
2.5-3 D. 2-2.58 - Correct Answer - A. 1-1.5
THE PHLEBOTOMY MASTERY EXAM: EVERYTHING YOU
NEED TO KNOW! EXAM AND PRACTICE EXAM 2025/26 250
QUESTIONS
A drug known to interfere with urine tests should be discontinued 48-72 hours prior to the
test. A. True B. False - Correct Answer - A. True
Which WBC comprises 40%-60% of the white blood cells? A. Lymphocytes B. Neutrophils
C. Monocytes D. Basophils - Correct Answer - B. Neutrophils
How many hours before a blood test should a patient discontinue the use of medications
known to interfere with blood tests? A. 1-4 B. 4-24 C. 24-36 D. 48-72 - Correct Answer - B.
4-24
Which tube is drawn last in a standard venipuncture procedure? A. Blood culture tube B.
Lavender C. Light-blue D. Red - Correct Answer - B. Lavender
Where do blood cells originate? A. Heart B. Lungs C. Bone marrow D. Capillaries - Correct
Answer - C. Bone marrow
Which blood vessel is NOT part of systemic circulation? A. Brachial artery B. Cephalic vein
C. Pulmonary artery D. Vena cava - Correct Answer - C. Pulmonary artery
In the order of draw for capillary specimens should you draw tubes with or without
additives first? A. Without B. With - Correct Answer - B. With
What is a condition that could cause leukopenia? A. Chemotherapy B. Leukemia C. Low
blood sugar D. Coagulation - Correct Answer - B. Leukemia
An example of a nosocomial infection is A. Catheter site of ICU patient becomes infected
B. Healthcare worker contracts HBV from a needlestick C. Patient is admitted with chicken
pox D. Patient breaks out with measles one day after admission - Correct Answer - A.
Catheter site of ICU patient becomes infected
Lymph originates from A. Joint fluid B. Plasma C. Serum D. Tissue fluid - Correct Answer -
D. Tissue fluid
,2 of 34
Which vein in the antecubital fossa is usually easiest to palpate in an obese patient? A.
Median cubital B. Cephalic C. Basilic D. Ulnar - Correct Answer - B. Cephalic
What organ would a doctor be intested in if he ordered an ALP, AST, ALT, GTT and bilirubin?
A. Heart B. Liver C. Kidney D. Spleen - Correct Answer - B. Liver
Which fluid is obtained by lumbar puncture. A. Peritoneal B. Pleural C. Cerebrospinal D.
Synovial - Correct Answer - C. Cerebrospinal
How many times should a red top tube be inverted? A. 2 B. 5 C. 0 D. 8 - Correct Answer - C.
0
Capillary puncture reference ranges are higher for this analyte. A. Calcium B. Glucose C.
Phosphorus D. Protein - Correct Answer - B. Glucose
If someone is cyanotic their skin may appear ____________. A. Flushed B. Clammy C. Bluish
D. Yellow/orange - Correct Answer - C. Bluish
Which is NOT a common symptom of shock? A. Clammy skin B. Weak pulse C. Blinking
eyes D. Shallow breathing - Correct Answer - C. Blinking eyes
The OGTT test diagnoses ______________. A. High blood pressure B. Cholesterol C.
Diabetes D. Hemoglobin - Correct Answer - C. Diabetes
Serum is depleted of fibrogen. A. True B. False - Correct Answer - A. True
Latex sensitivity is an allergic reaction. A. True B. False - Correct Answer - A. True
There is a 9 to 1 ratio of blood to anticoagulant in a light blue tube. A. True B. False - Correct
Answer - A. True
Which is the best tube for collecting an ethanol specimen? A. EDTA B. Heparin C. Sodium
citrate D. Sodium fluoride - Correct Answer - D. Sodium fluoride
(gray top)
Glass particle in serum seperator tubes A. Deter coagulation B. Enhance clotting C. Inhibit
glycolysis D. Prevent hemolysis - Correct Answer - B. Enhance clotting
Which element prevents glycolysis? A. EDTA B. Heparin C. Sodium Fluoride D. Potassium
oxalate - Correct Answer - D. Potassium oxalate
,3 of 34
What kind of blood do arteries and arterioles carry? A. Deoxygenated B. Oxygenated -
Correct Answer - B. Oxygenated
Blood cultures are collected in a ___________ tube. A. Red B. Yellow C. Blue D. Green -
Correct Answer - B. Yellow
The Good Samaritan Law allows health care workers to provide medical care above and
beyond their normal field without being sued. A. True B. False - Correct Answer - B. False
Blood cultures are used to diagnose ________________. A. Diabetes B. FUO C. FAQ D. PT -
Correct Answer - B. FUO
What is the preferred antiseptic for venipuncture? A. Betadine B. Iodine C. Isopropyl
alcohol D. Jack Daniels - Correct Answer - C. Isopropyl alcohol
The following is a test of the respiratory system A. ABGs B. CSF C. ETOH D. UA - Correct
Answer - A. ABGs
What part of the infant is a PKU test performed on? A. Toe B. Finger C. Heel D. Forehead -
Correct Answer - C. Heel
The myocardium is the layer of the heart that is made of ___________-. A. Muscle B.
Cartilage C. Endometrial tissue - Correct Answer - A. Muscle
Which is not a PPE? A. Mask B. Goggles C. Room dividers D. Gloves - Correct Answer - C.
Room dividers
To check for trace elements a phebotomist would use a ______ tube. A. Light blue B. Royal
blue C. Tiger top D. Yellow - Correct Answer - B. Royal blue
The following were collected for a routine venipuncture. Which item is incorrect? A. Tubes
B. Iodine swab C. Touniquet D. Safety needles - Correct Answer - B. Iodine swab
What causes vacuum tubes to fill with blood? A. Arterial blood pressure B. Patient fist
pumping C. Tourniquet pressure D. Tube vacuum - Correct Answer - D. Tube vacuum
Which laboratory section performs blood pregnancy tests? A. Chemistry B. Hematology C.
Serology D. Blood bank - Correct Answer - A. Chemistry
What special handling does an ABG require? A. Warming B. Chilling C. Shaking D. Pre-
warmed tubes - Correct Answer - B. Chilling
, 4 of 34
Using a butterfly may be necessary if the vein collapses with the evacuated tube system. A.
True B. False - Correct Answer - A. True
Arteries have valves that prevent the blood from flowing back. A. True B. False - Correct
Answer - B. False
Lipemic bloodserum appears: A. Bluish B. Milky C. Purple D. Thick - Correct Answer - B.
Milky
Microorganism cultures are collected in _____________tubes. A. Light-blue B. Royal blue C.
Green D. Yellow - Correct Answer - D. Yellow
Which of the following specimens are least likely to require special handling? A. Bilirubin
and Folate B. Cholesterol and Uric Acid C. Gastrin and Lactic Acid D. Homocystine and
renin - Correct Answer - B. Cholesterol and Uric Acid
A pad used to protect the patient's clothing. A. Flux B. Chux C. Tourniquet D. Antiseptic -
Correct Answer - B. Chux
The two circulations of the heart are _________and ___________. A. Tricuspid and Bicuspid
B. Pulmonary and Systemic C. Right Ventricle and Left Ventricle D. Right Atrium and Left
Atrium - Correct Answer - B. Pulmonary and Systemic
What are the 3 components of a urinalysis? A. Physical, chemical, microscopic exams B.
Gravity, clarity and color exams C. Blood, protein and glucose exams D. Hear, see and
taste exam - Correct Answer - A. Physical, chemical, microscopic exams
When you explain the venipuncture procedure to the patient and they agree to the test it is
called _______. A. Getting the blood B. Informed consent C. Constant talking D. HIPAA -
Correct Answer - B. Informed consent
At what intervals should blood be clotted during a bleeding time test? A. 15 seconds B. 20
seconds C. 30 seconds D. 35 seconds - Correct Answer - C. 30 seconds
Contact, droplet and airborne are ____________. A. Modes of transmission B. Portals of exit
C. Portals of entry D. Agents - Correct Answer - A. Modes of transmission
What is the size (in inches) of the needle routinely used for venipuncture? A. 1-1.5 B. 2-3 C.
2.5-3 D. 2-2.58 - Correct Answer - A. 1-1.5
