BIOL 2261L Unit 2: Aseptic Technique & Culturing (LAB)
Questions With Complete Solutions
Absent surface growth Correct Answer No surface growth
Agar Correct Answer A polysaccharide from seaweed; Most
commonly used gelling agent
Aseptic technique Correct Answer A way to manipulate
cultures to minimize the chance of contamination
Autoclaving Correct Answer A process of sterilization which
heats the medium to 121 degrees Celsius under 15 pounds per
square inch (PSI) above atmospheric pressure for a minimum of
15 minutes
Autotrophs Correct Answer Category of chemotrophs;
organisms that utilize carbon dioxide as their sole organic
carbon source to build organic molecules
Broth media Correct Answer Consists of nutrients dissolved
in water
Examples: Trypticase soy broth (TSB) and nutrient broth
Chemotrophs Correct Answer Must oxidize inorganic or
organic chemicals for energy
Colony color: pigments Correct Answer Soluble substances
secreted by bacteria that change the color of the medium
surrounding the colonies
, Colony elevation Correct Answer Refers to how the colony
rises away from the surface of the medium
Colony elevation: convex Correct Answer Curves across the
top, much like a drop of water
Colony elevation: crateriform Correct Answer Has a
depression in the center of the colony
Colony elevation: flat Correct Answer Lies completely flat
along the surface of the medium
Colony elevation: hilly Correct Answer Has an irregular
surface with many elevations and depressions
Colony elevation: pulvinate Correct Answer Similar to
convex, but is higher off the plate
Colony elevation: raised Correct Answer Has its top above
the medium but still parallel with the surface
Colony elevation: umbonate Correct Answer Has a higher
center than the edges
Colony margin Correct Answer The edge of a bacterial
colony
Colony margin: curled Correct Answer Form concentric rings
of growth
Questions With Complete Solutions
Absent surface growth Correct Answer No surface growth
Agar Correct Answer A polysaccharide from seaweed; Most
commonly used gelling agent
Aseptic technique Correct Answer A way to manipulate
cultures to minimize the chance of contamination
Autoclaving Correct Answer A process of sterilization which
heats the medium to 121 degrees Celsius under 15 pounds per
square inch (PSI) above atmospheric pressure for a minimum of
15 minutes
Autotrophs Correct Answer Category of chemotrophs;
organisms that utilize carbon dioxide as their sole organic
carbon source to build organic molecules
Broth media Correct Answer Consists of nutrients dissolved
in water
Examples: Trypticase soy broth (TSB) and nutrient broth
Chemotrophs Correct Answer Must oxidize inorganic or
organic chemicals for energy
Colony color: pigments Correct Answer Soluble substances
secreted by bacteria that change the color of the medium
surrounding the colonies
, Colony elevation Correct Answer Refers to how the colony
rises away from the surface of the medium
Colony elevation: convex Correct Answer Curves across the
top, much like a drop of water
Colony elevation: crateriform Correct Answer Has a
depression in the center of the colony
Colony elevation: flat Correct Answer Lies completely flat
along the surface of the medium
Colony elevation: hilly Correct Answer Has an irregular
surface with many elevations and depressions
Colony elevation: pulvinate Correct Answer Similar to
convex, but is higher off the plate
Colony elevation: raised Correct Answer Has its top above
the medium but still parallel with the surface
Colony elevation: umbonate Correct Answer Has a higher
center than the edges
Colony margin Correct Answer The edge of a bacterial
colony
Colony margin: curled Correct Answer Form concentric rings
of growth
