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NHA PHLEBOTOMY CERTIFICATION EXAM 2025–2026
TEST BANK | 100+ PRACTICE QUESTIONS WITH 100%
CORRECT ANSWERS & RATIONALES
Topic: Phlebotomy Basics
Q1. Which vein is most used for venipuncture?
A. Basilic vein
B. Cephalic vein
C. Median cubital vein
D. Radial vein
Correct Answer: C. Median cubital vein
Rationale: The median cubital vein is preferred because it is large, stable, and has fewer
complications.
Q2. Which of the following PPE is most essential during venipuncture?
A. Face shield
B. Gloves
C. Gown
D. Shoe covers
Correct Answer: B. Gloves
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Rationale: Gloves are mandatory to protect both patient and phlebotomist from
bloodborne pathogens.
Q3. The order of draw for blood collection begins with:
A. EDTA tube
B. Blood culture bottles
C. Serum separator tube
D. Heparin tube
Correct Answer: B. Blood culture bottles
Rationale: Blood cultures are collected first to avoid contamination.
Q4. What is the correct angle for needle insertion in antecubital venipuncture?
A. 15–30 degrees
B. 45 degrees
C. 5–10 degrees
D. 60 degrees
Correct Answer: A. 15–30 degrees
Rationale: This angle ensures smooth entry into the vein without piercing through it.
Q5. A lavender-top tube contains which additive?
A. Heparin
B. Citrate
C. EDTA
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D. No additive
Correct Answer: C. EDTA
Rationale: Lavender tubes contain EDTA for hematology tests (CBC, blood smears).
Topic: Safety & Infection Control
Q6. Which of the following is an OSHA requirement for sharps disposal?
A. Discard in a plastic bag
B. Recap the needle before disposal
C. Use puncture-resistant sharps container
D. Dispose in the trash can
Correct Answer: C. Use puncture-resistant sharps container
Rationale: OSHA requires immediate disposal into puncture-resistant, labeled sharps
containers.
Q7. The proper hand hygiene technique before venipuncture is:
A. Rinse with water only
B. Use alcohol-based hand rub or wash with soap and water
C. Wear gloves without washing
D. Apply lotion only
Correct Answer: B. Use alcohol-based hand rub or wash with soap and water
Rationale: Hand hygiene reduces risk of infection transmission.
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Q8. Which bloodborne pathogen poses the greatest occupational risk to healthcare
workers?
A. Hepatitis A
B. Hepatitis B
C. HIV
D. Influenza
Correct Answer: B. Hepatitis B
Rationale: Hepatitis B is more infectious than HIV and easily transmitted through
needlesticks.
Q9. What should be done if a patient faints during venipuncture?
A. Continue drawing blood quickly
B. Hold pressure at site and lower patient’s head
C. Leave the patient unattended
D. Remove gloves immediately
Correct Answer: B. Hold pressure at site and lower patient’s head
Rationale: Stopping the draw and positioning the patient safely prevents injury.
Q10. The most effective way to break the chain of infection is:
A. Wearing masks
B. Hand hygiene
C. Isolation
NHA PHLEBOTOMY CERTIFICATION EXAM 2025–2026
TEST BANK | 100+ PRACTICE QUESTIONS WITH 100%
CORRECT ANSWERS & RATIONALES
Topic: Phlebotomy Basics
Q1. Which vein is most used for venipuncture?
A. Basilic vein
B. Cephalic vein
C. Median cubital vein
D. Radial vein
Correct Answer: C. Median cubital vein
Rationale: The median cubital vein is preferred because it is large, stable, and has fewer
complications.
Q2. Which of the following PPE is most essential during venipuncture?
A. Face shield
B. Gloves
C. Gown
D. Shoe covers
Correct Answer: B. Gloves
,2|Page
Rationale: Gloves are mandatory to protect both patient and phlebotomist from
bloodborne pathogens.
Q3. The order of draw for blood collection begins with:
A. EDTA tube
B. Blood culture bottles
C. Serum separator tube
D. Heparin tube
Correct Answer: B. Blood culture bottles
Rationale: Blood cultures are collected first to avoid contamination.
Q4. What is the correct angle for needle insertion in antecubital venipuncture?
A. 15–30 degrees
B. 45 degrees
C. 5–10 degrees
D. 60 degrees
Correct Answer: A. 15–30 degrees
Rationale: This angle ensures smooth entry into the vein without piercing through it.
Q5. A lavender-top tube contains which additive?
A. Heparin
B. Citrate
C. EDTA
,3|Page
D. No additive
Correct Answer: C. EDTA
Rationale: Lavender tubes contain EDTA for hematology tests (CBC, blood smears).
Topic: Safety & Infection Control
Q6. Which of the following is an OSHA requirement for sharps disposal?
A. Discard in a plastic bag
B. Recap the needle before disposal
C. Use puncture-resistant sharps container
D. Dispose in the trash can
Correct Answer: C. Use puncture-resistant sharps container
Rationale: OSHA requires immediate disposal into puncture-resistant, labeled sharps
containers.
Q7. The proper hand hygiene technique before venipuncture is:
A. Rinse with water only
B. Use alcohol-based hand rub or wash with soap and water
C. Wear gloves without washing
D. Apply lotion only
Correct Answer: B. Use alcohol-based hand rub or wash with soap and water
Rationale: Hand hygiene reduces risk of infection transmission.
, 4|Page
Q8. Which bloodborne pathogen poses the greatest occupational risk to healthcare
workers?
A. Hepatitis A
B. Hepatitis B
C. HIV
D. Influenza
Correct Answer: B. Hepatitis B
Rationale: Hepatitis B is more infectious than HIV and easily transmitted through
needlesticks.
Q9. What should be done if a patient faints during venipuncture?
A. Continue drawing blood quickly
B. Hold pressure at site and lower patient’s head
C. Leave the patient unattended
D. Remove gloves immediately
Correct Answer: B. Hold pressure at site and lower patient’s head
Rationale: Stopping the draw and positioning the patient safely prevents injury.
Q10. The most effective way to break the chain of infection is:
A. Wearing masks
B. Hand hygiene
C. Isolation
