PHARM LAB EXAM QUESTIONS AND
ANSWERS
What are target based assays good for? - ANSWER-- enzyme inhibitors
- receptor agonists and antagonists
- protein-protein interactions
Cell based assay - ANSWER-Effect on the cell being studied may lead. to improvement
of disease symptoms
What are cell based assays good for? - ANSWER-- in finding new drug candidates
against cancer and infectious diseases
- used as secondary assays following initial target based assay
- must pass the cell membrane and find the target in a more "natural" environment
A series for 10,000 different compounds is screened for activity against a drug resistant
strain of the Staphylococcus aureus bacterium. This an example of
A. Target based assay
B. Whole cell assay
C. Lead optimization
D. Preclinical toxicology evaluation - ANSWER-B. Whole cell assay
Testing against the whole bacteria. If target based would be testing a specific enzyme
What percent of known small molecule drugs are natural products or semisynthetic
compounds - ANSWER-1/3
Natural products can be quite __________ and structurally _________ - ANSWER-
complex, unique
What are the steps in identifying biologically active natural products - ANSWER--
identification and collection of plants or microorganisms
- bioassay-guided fractionation
- determination of the structure of active compounds
Who are known to collect known compounds into libraries - ANSWER-pharmaceutical
companies, chemical suppliers, academic labs
How are new compounds for libraries obtained - ANSWER-- traditional synthetic
methods
- combinatorial synthesis
- identification of new compounds from nature
ANSWERS
What are target based assays good for? - ANSWER-- enzyme inhibitors
- receptor agonists and antagonists
- protein-protein interactions
Cell based assay - ANSWER-Effect on the cell being studied may lead. to improvement
of disease symptoms
What are cell based assays good for? - ANSWER-- in finding new drug candidates
against cancer and infectious diseases
- used as secondary assays following initial target based assay
- must pass the cell membrane and find the target in a more "natural" environment
A series for 10,000 different compounds is screened for activity against a drug resistant
strain of the Staphylococcus aureus bacterium. This an example of
A. Target based assay
B. Whole cell assay
C. Lead optimization
D. Preclinical toxicology evaluation - ANSWER-B. Whole cell assay
Testing against the whole bacteria. If target based would be testing a specific enzyme
What percent of known small molecule drugs are natural products or semisynthetic
compounds - ANSWER-1/3
Natural products can be quite __________ and structurally _________ - ANSWER-
complex, unique
What are the steps in identifying biologically active natural products - ANSWER--
identification and collection of plants or microorganisms
- bioassay-guided fractionation
- determination of the structure of active compounds
Who are known to collect known compounds into libraries - ANSWER-pharmaceutical
companies, chemical suppliers, academic labs
How are new compounds for libraries obtained - ANSWER-- traditional synthetic
methods
- combinatorial synthesis
- identification of new compounds from nature
