Medical Interventions 4.1.2 Protein Factories
questions and answersMedical Interventions 4.1.2
Protein Factories questions and answers
Define plasmid - (ANSWERS)A plasmid is a small loop/ring of circular DNA
What are 3 reasons why plasmids and bacterial cells are a helpful tool in genetic engineering?
- (ANSWERS)1) one could add genes to a plasmid without ruining the rest of the genome.
2) plasmids can be cut and have extra DNA sequences added to it, with no damage and can
connect back together after.
3) bacterial cells will reproduce what the plasmid is carrying making more of that gene.
What are the steps of using recombinant DNA techniques and plasmids to produce human
insulin? - (ANSWERS)1) scientists build the human insulin gene in the laboratory.
2) they then remove a loop of bacterial DNA known as the plasmid
3) they then insert the human insulin gene into the plasmid
4) researchers return the plasmid to the bacteria
5) they put the recombinant bacteria in large fermentation tanks
6) in the tanks, the bacteria begin producing human insulin
7) scientists harvest the insulin made by the bacteria
8) the insulin is then purified for use.
What is GFP used for? - (ANSWERS)A visual marker
What is transformation? - (ANSWERS)The uptake of foreign DNA, often a circular plasmid.
What is a plasmid? - (ANSWERS)circular piece of autonomously replicating DNA who was
originally evolved by bacteria, and can express the antibiotic resistant gene or can be modified
to express proteins of interest.
What are some methods of transformation? - (ANSWERS)Electroporation, electric shock (
makes cell membranes permeable to DNA), and calcium chloride/heat shock.
What is the formula for transformation efficiency? - (ANSWERS)# of cells growing on a
plate/amount of DNA on a plate
What are three useful applications of GFP in cellular biology and medical science? -
(ANSWERS)Research of diseases, tracking progress of medicines, and for research
How are restriction enzymes and ligaments utilized in recombinant DNA technology? -
(ANSWERS)Restriction enzymes cut the DNA strand and ligase glues it back together.
questions and answersMedical Interventions 4.1.2
Protein Factories questions and answers
Define plasmid - (ANSWERS)A plasmid is a small loop/ring of circular DNA
What are 3 reasons why plasmids and bacterial cells are a helpful tool in genetic engineering?
- (ANSWERS)1) one could add genes to a plasmid without ruining the rest of the genome.
2) plasmids can be cut and have extra DNA sequences added to it, with no damage and can
connect back together after.
3) bacterial cells will reproduce what the plasmid is carrying making more of that gene.
What are the steps of using recombinant DNA techniques and plasmids to produce human
insulin? - (ANSWERS)1) scientists build the human insulin gene in the laboratory.
2) they then remove a loop of bacterial DNA known as the plasmid
3) they then insert the human insulin gene into the plasmid
4) researchers return the plasmid to the bacteria
5) they put the recombinant bacteria in large fermentation tanks
6) in the tanks, the bacteria begin producing human insulin
7) scientists harvest the insulin made by the bacteria
8) the insulin is then purified for use.
What is GFP used for? - (ANSWERS)A visual marker
What is transformation? - (ANSWERS)The uptake of foreign DNA, often a circular plasmid.
What is a plasmid? - (ANSWERS)circular piece of autonomously replicating DNA who was
originally evolved by bacteria, and can express the antibiotic resistant gene or can be modified
to express proteins of interest.
What are some methods of transformation? - (ANSWERS)Electroporation, electric shock (
makes cell membranes permeable to DNA), and calcium chloride/heat shock.
What is the formula for transformation efficiency? - (ANSWERS)# of cells growing on a
plate/amount of DNA on a plate
What are three useful applications of GFP in cellular biology and medical science? -
(ANSWERS)Research of diseases, tracking progress of medicines, and for research
How are restriction enzymes and ligaments utilized in recombinant DNA technology? -
(ANSWERS)Restriction enzymes cut the DNA strand and ligase glues it back together.
