Week 5 Lab
Instructions
Urinalysis
Activity Deliverable Points
Part A Urinalysis 30
All Lab Deliverables Complete Part A 30
References:
1. Carolina Distance Learning
Part A: Digestion of Proteins
1.0 —Read through the entire laboratory instructions and ask for clarification from your instructor.
1. —Acquire synthetic urine as described in the preparation section of the instructions.
2. —Conduct Activity 1 and fill in Data Table 1- Specific Gravity of Urine.
3. —Conduct Activity 3 and fill in Data Table 2- Urine Composition.
4. —Conduct Activity 4 and fill in Data Table 3- Urine Production
5. —When complete, upload your week 4 lab exercise into Canvas.
Submit Parts A Deliverables
Refer to the syllabus section, "Due Dates for Assignments & Exams" for due date information.
Activity Deliverable Points
Part A Urinalysis 30
All Lab Deliverables Complete Part A 30
BIOS256 Week 4 Lab Instructions Page 1 of 11
, Materials
· Carolina Urinalysis kit
Activity 1: Measuring Specific Gravity Using a Hydrometer
1. Place a clean paper towel on your work surface. Set the hydrometer jar on the paper towel.
2. Select one of the two simulated urine samples and pour some of the sample into the jar of
the hydrometer to approximately 3 cm from the top.
3. Hold the hydrometer by the stem (the slender portion with the specific gravity scale), and
carefully lower it into the jar. Give it a slight, gentle spin as you release the hydrometer. It should
float in the sample. Do not force the hydrometer deeper into the sample because if too much
fluid adheres to the stem, the reading may be inaccurate.
4. Read the specific gravity scale using the meniscus (concave surface) of the sample. Determine
which line on the hydrometer intersects with the lowest point of the meniscus. Record the specific
gravity readings in Data Table 1.
5. Remove the hydrometer from the jar, rinse it thoroughly with tap water, and dry it with a paper
towel. Empty the contents of the jar down the drain. Rinse the jar thoroughly with tap water
and dry it inside and out with a paper towel.
1. Repeat Steps 1–5 for the other simulated urine sample.
Activity 2: Determining Urine Composition
1. Place two paper cups a paper towel: label one “simulated normal” and the other
“simulated unknown.”
2. Ensure that each cup is about ¾ full.
3. Remove a fresh test strip from the packet, taking care to touch only the plastic and not the
raised test surfaces.
4. Working with one sample at a time, briefly immerse the strip in the first sample so that all
test surfaces are submerged.
5. Remove the strip from the sample and do not allow the strip to come into contact with the
cup. Carefully tap the plastic of the test strip to remove any excess urine.
6. Turn the strip onto its edge (not the test surfaces) and tap it once on the paper towel to remove
any remaining excess sample. Do not wipe the strip dry. Let the strip rest, test surfaces facing up,
on the paper towel.
7. Start your timer, and after 60 seconds, compare the strip to Figure 1 and the Interpretation
Guide found below. Record your results quickly in Data Table 2.
8. Continue timing for an additional 60 seconds (120 seconds total). Compare the strip to Figure 2
and Table 1 with respect to the leukocyte test. If there has been a change since the 60-second
reading, record the results in Data Table 2.
9. Repeat the procedure for the other sample, using a fresh test strip.
10. Dispose of the samples in the lab sink.
BIOS256 Week 4 Lab Instructions Page 2 of 11
Instructions
Urinalysis
Activity Deliverable Points
Part A Urinalysis 30
All Lab Deliverables Complete Part A 30
References:
1. Carolina Distance Learning
Part A: Digestion of Proteins
1.0 —Read through the entire laboratory instructions and ask for clarification from your instructor.
1. —Acquire synthetic urine as described in the preparation section of the instructions.
2. —Conduct Activity 1 and fill in Data Table 1- Specific Gravity of Urine.
3. —Conduct Activity 3 and fill in Data Table 2- Urine Composition.
4. —Conduct Activity 4 and fill in Data Table 3- Urine Production
5. —When complete, upload your week 4 lab exercise into Canvas.
Submit Parts A Deliverables
Refer to the syllabus section, "Due Dates for Assignments & Exams" for due date information.
Activity Deliverable Points
Part A Urinalysis 30
All Lab Deliverables Complete Part A 30
BIOS256 Week 4 Lab Instructions Page 1 of 11
, Materials
· Carolina Urinalysis kit
Activity 1: Measuring Specific Gravity Using a Hydrometer
1. Place a clean paper towel on your work surface. Set the hydrometer jar on the paper towel.
2. Select one of the two simulated urine samples and pour some of the sample into the jar of
the hydrometer to approximately 3 cm from the top.
3. Hold the hydrometer by the stem (the slender portion with the specific gravity scale), and
carefully lower it into the jar. Give it a slight, gentle spin as you release the hydrometer. It should
float in the sample. Do not force the hydrometer deeper into the sample because if too much
fluid adheres to the stem, the reading may be inaccurate.
4. Read the specific gravity scale using the meniscus (concave surface) of the sample. Determine
which line on the hydrometer intersects with the lowest point of the meniscus. Record the specific
gravity readings in Data Table 1.
5. Remove the hydrometer from the jar, rinse it thoroughly with tap water, and dry it with a paper
towel. Empty the contents of the jar down the drain. Rinse the jar thoroughly with tap water
and dry it inside and out with a paper towel.
1. Repeat Steps 1–5 for the other simulated urine sample.
Activity 2: Determining Urine Composition
1. Place two paper cups a paper towel: label one “simulated normal” and the other
“simulated unknown.”
2. Ensure that each cup is about ¾ full.
3. Remove a fresh test strip from the packet, taking care to touch only the plastic and not the
raised test surfaces.
4. Working with one sample at a time, briefly immerse the strip in the first sample so that all
test surfaces are submerged.
5. Remove the strip from the sample and do not allow the strip to come into contact with the
cup. Carefully tap the plastic of the test strip to remove any excess urine.
6. Turn the strip onto its edge (not the test surfaces) and tap it once on the paper towel to remove
any remaining excess sample. Do not wipe the strip dry. Let the strip rest, test surfaces facing up,
on the paper towel.
7. Start your timer, and after 60 seconds, compare the strip to Figure 1 and the Interpretation
Guide found below. Record your results quickly in Data Table 2.
8. Continue timing for an additional 60 seconds (120 seconds total). Compare the strip to Figure 2
and Table 1 with respect to the leukocyte test. If there has been a change since the 60-second
reading, record the results in Data Table 2.
9. Repeat the procedure for the other sample, using a fresh test strip.
10. Dispose of the samples in the lab sink.
BIOS256 Week 4 Lab Instructions Page 2 of 11
