Page 1
ASCP MLS EXAM 2 PRACTICE
QUESTIONS WITH VERIFIED SOLUTIONS
NEW MODIFIED EXAM GRADED A+
Patients with antibody to the following antigen are immune to Hepatitis B: --
ANSWER--Surface antigen (HbSAg)
Which two of the following tests are helpful for documenting previous
Streptococcal throat and skin infections: -- ANSWER--ASO titer and Anti-
DNase B
In mass spectrometry, the "appearance potential" is defined as the: -- ANSWER-
-Minimum energy required for the appearance of a particular fragment ion in
mass spectrum
What is generally accepted as the lower threshold value for semen pH from
fertile males? -- ANSWER--7.2
Coulometry: -- ANSWER--An automated method for measuring chloride which
generates silver ions in the reaction
Page | 1
, Page 2
Erythroleukemia: -- ANSWER--Also known as Acute Myelogenous Leukemia
type M6. This type is associated with either the presence of both erythroid and
myeloid precursors OR strictly erythroid precursors.
Myelomonocytic leukemia -- ANSWER--Is associated with an increase in
myeloid and monocytic cell precursors, but not red blood cell precursors
Key identifying characteristic for Pantoa (Enterobacter) agglomerants: --
ANSWER--negative reactions for the three decarboxylases commonly tested (
Lysine, arginine and ornithine)
Which organism is MOST likely responsible for septic reactions associated with
Red Blood Cell transfusions? -- ANSWER--Yersina entercolitica is most likely
responsible for septic reactions in transfusions of Red Blood Cells. This
organism is usually acquired by ingestion of contaminated food and causes mild
symptoms of abdominal pain and diarrhea. Growth of Y. entercolitica is
enhanced in iron-rich environments such as red cell components.
Gaucher's disease -- ANSWER--The most common lysosomal storage disease
caused by insufficient activity of the lysosomal enzyme glucocerebrosidase that
leads to the deposition of glucocerebroside in cells of the macrophage-monocyte
system.
Page | 2
, Page 3
Diseases associated with dysfunctions of polymorphonuclear neutrophils
(PMNs) -- ANSWER--Chediak-Higashi syndrome (CH), Chronic
granulomatous disease (CGD), Myeloperoxidase deficiency (MPO) Nephrotic
Syndrome: -- ANSWER--Caused by damage to the kidneys, especially the
basement membrane of the glomerulus; which causes abnormal excretion of
protein and red blood cells in the urine. Fats are also present in the urine in most
cases. A foamy appearance of the urine is a key characteristic of this condition.
Clinical symptoms include:
Swelling
Weight gain from fluid retention
Poor appetite
High blood pressure
Key Biochemical reactions for Enterobacteriaceae: -- ANSWER--The key
biochemical reactions by which the family Enterobacteriaceae can be identified
include fermentation of carbohydrates, reduction of nitrates to nitrites and the
absence of cytochrome oxidase activity.
What feature is most often found with patients with a splenectomy on a Wright's
stained blood smear? -- ANSWER--Howell-Jolly bodies
Species that are Lysine (+), arginine (=) and ornithine (+): -- ANSWER--
Enterobacter aerogenes, Edwardsiella species and most Serratia species
Page | 3
, Page 4
Lysine reaction for all Citrobacter: -- ANSWER--Negative
ANA -- ANSWER--See 2 quiz #27
Prozone effect: -- ANSWER--Results in a false positive reaction; is the result of
antibody excess and dilution of antibody can help prevent its occurrence
Purpose of a bi-chromatic analyzer with dual wavelengths is: -- ANSWER-
Minimize the effect of interference
Key characteristics of Micrococcus: -- ANSWER--Susceptible to bacitracin
("A") and resistant to furazolidone; gram positive cocci in tetras, bacteria cells
of micrococcus luteus are larger than those of the staphylococci and can arrange
in tetras
What is the glomerular filtration rate for a patient with a serum creatinine of 2
mg/dL, if the urine creatinine was 124 mg/dL and the urine volume was 2.2
L/24 hrs? -- ANSWER--95 mL/min
One method of calculating a glomerular filtration rate is using creatinine and
urine volume to determine creatinine clearance. The equation is as follows:
Creatinine Clearance = (urine creatinine X urine flow rate) / plasma creatinine;
where urine flow rate = volume in mL /24 hours x h/60 min)
Page | 4
ASCP MLS EXAM 2 PRACTICE
QUESTIONS WITH VERIFIED SOLUTIONS
NEW MODIFIED EXAM GRADED A+
Patients with antibody to the following antigen are immune to Hepatitis B: --
ANSWER--Surface antigen (HbSAg)
Which two of the following tests are helpful for documenting previous
Streptococcal throat and skin infections: -- ANSWER--ASO titer and Anti-
DNase B
In mass spectrometry, the "appearance potential" is defined as the: -- ANSWER-
-Minimum energy required for the appearance of a particular fragment ion in
mass spectrum
What is generally accepted as the lower threshold value for semen pH from
fertile males? -- ANSWER--7.2
Coulometry: -- ANSWER--An automated method for measuring chloride which
generates silver ions in the reaction
Page | 1
, Page 2
Erythroleukemia: -- ANSWER--Also known as Acute Myelogenous Leukemia
type M6. This type is associated with either the presence of both erythroid and
myeloid precursors OR strictly erythroid precursors.
Myelomonocytic leukemia -- ANSWER--Is associated with an increase in
myeloid and monocytic cell precursors, but not red blood cell precursors
Key identifying characteristic for Pantoa (Enterobacter) agglomerants: --
ANSWER--negative reactions for the three decarboxylases commonly tested (
Lysine, arginine and ornithine)
Which organism is MOST likely responsible for septic reactions associated with
Red Blood Cell transfusions? -- ANSWER--Yersina entercolitica is most likely
responsible for septic reactions in transfusions of Red Blood Cells. This
organism is usually acquired by ingestion of contaminated food and causes mild
symptoms of abdominal pain and diarrhea. Growth of Y. entercolitica is
enhanced in iron-rich environments such as red cell components.
Gaucher's disease -- ANSWER--The most common lysosomal storage disease
caused by insufficient activity of the lysosomal enzyme glucocerebrosidase that
leads to the deposition of glucocerebroside in cells of the macrophage-monocyte
system.
Page | 2
, Page 3
Diseases associated with dysfunctions of polymorphonuclear neutrophils
(PMNs) -- ANSWER--Chediak-Higashi syndrome (CH), Chronic
granulomatous disease (CGD), Myeloperoxidase deficiency (MPO) Nephrotic
Syndrome: -- ANSWER--Caused by damage to the kidneys, especially the
basement membrane of the glomerulus; which causes abnormal excretion of
protein and red blood cells in the urine. Fats are also present in the urine in most
cases. A foamy appearance of the urine is a key characteristic of this condition.
Clinical symptoms include:
Swelling
Weight gain from fluid retention
Poor appetite
High blood pressure
Key Biochemical reactions for Enterobacteriaceae: -- ANSWER--The key
biochemical reactions by which the family Enterobacteriaceae can be identified
include fermentation of carbohydrates, reduction of nitrates to nitrites and the
absence of cytochrome oxidase activity.
What feature is most often found with patients with a splenectomy on a Wright's
stained blood smear? -- ANSWER--Howell-Jolly bodies
Species that are Lysine (+), arginine (=) and ornithine (+): -- ANSWER--
Enterobacter aerogenes, Edwardsiella species and most Serratia species
Page | 3
, Page 4
Lysine reaction for all Citrobacter: -- ANSWER--Negative
ANA -- ANSWER--See 2 quiz #27
Prozone effect: -- ANSWER--Results in a false positive reaction; is the result of
antibody excess and dilution of antibody can help prevent its occurrence
Purpose of a bi-chromatic analyzer with dual wavelengths is: -- ANSWER-
Minimize the effect of interference
Key characteristics of Micrococcus: -- ANSWER--Susceptible to bacitracin
("A") and resistant to furazolidone; gram positive cocci in tetras, bacteria cells
of micrococcus luteus are larger than those of the staphylococci and can arrange
in tetras
What is the glomerular filtration rate for a patient with a serum creatinine of 2
mg/dL, if the urine creatinine was 124 mg/dL and the urine volume was 2.2
L/24 hrs? -- ANSWER--95 mL/min
One method of calculating a glomerular filtration rate is using creatinine and
urine volume to determine creatinine clearance. The equation is as follows:
Creatinine Clearance = (urine creatinine X urine flow rate) / plasma creatinine;
where urine flow rate = volume in mL /24 hours x h/60 min)
Page | 4
