BMB 402 Exam #2 Questions And Answers {100% correct}
Latest Update
1. Glycogen Polymer of about 50,000 glucose residues with
about 2,000 nonreducing ends. (makes up about
10% of a liver cell)
2. Glycogen phosphorylase Removes successive glucose residues until it reaches the
fourth
glucose unit from a branching point
3. How does glycogen unbranched polymer?
phos- phorylase work?
8. What enzyme converts G1P to th
4. Glycogen phosphorylase useful G6P form?
adds a phosphate
group, so does it need
to use ATP?
5. Once the glycogen
phos- phorylase
reaches four residue
from a branch- ing
point, which enzyme is
needed to continue to
free glucose from
glycogen?
6. How does the
debranching enzyme
work?
7. What kind of linkage
con- nects the branch
points vs the
, BMB 402 Exam #2 Questions And Answers {100% correct}
Latest Update
Adds a phosphate to a nonreducing end
of a glycogen chain to make a glucose
1-phosphate
Using its transferase activity, the debranching enzyme
No, glycogen phosphorylase uses a free takes 3 of the 4 branched glucose residues and adds
inorganic phosphate them to the end of the adjacent strand. Next, using its
glucosidase activity, it removes the last glucose residue
Branch points: alpha1->6
Debranching enzyme unbranched polymer: alpha1->4
phosphoglucomutase
9.
, BMB 402 Exam #2 Questions And Answers {100% correct}
Latest Update
How does phosphogluco- 1. A serine residue adds a phosphate group to
mutase work? carbon 6 produc- ing a G16P intermediate
2. Same serine residue removes the phosphate
group from car- bon 1
There is an element of chance in this reaction; sometimes it goes
the right direction sometimes not. With enough
permutations, the reaction will eventually end up
producing the intended prod- uct
10. What are the main substrate Sugar nucleotides (UDP-glucose)
for the polymerization of
glucose into glycogen?
11. Why sugar nucleotides? 1. Formation is metabolically irreversible
2. The nucleotide moiety has many groups that can
participate in non-covalent interactions with enzymes
3. The nucleotidyl group is an excellent leaving group
4. Tagged hexoses with nucleotidyl groups can be set
aside by the cell for just one purpose
12. How are sugar NDP-sugar phosphorylase catalyzes the nucleophillic attack
nucleotides formed? of the negative charge from phosphate on a sugar
phosphate to the first phosphate atom in an NTP molecule
(small positive delta G)
13. If there is a small and
pos- itive delta G for The reaction also produces pyrophosphate (PPi) which is
the forma- con- verted to inorganic phosphate. This reaction has a
large negative
tion of the sugar nucleotide, delta G from the fast removal of the product
what drives the reaction
14. Which enzyme catalyzes pyrophos- phate to inorganic
the conversion of phos- phate?
, BMB 402 Exam #2 Questions And Answers {100% correct}
Latest Update
inorganic phosphatase
Latest Update
1. Glycogen Polymer of about 50,000 glucose residues with
about 2,000 nonreducing ends. (makes up about
10% of a liver cell)
2. Glycogen phosphorylase Removes successive glucose residues until it reaches the
fourth
glucose unit from a branching point
3. How does glycogen unbranched polymer?
phos- phorylase work?
8. What enzyme converts G1P to th
4. Glycogen phosphorylase useful G6P form?
adds a phosphate
group, so does it need
to use ATP?
5. Once the glycogen
phos- phorylase
reaches four residue
from a branch- ing
point, which enzyme is
needed to continue to
free glucose from
glycogen?
6. How does the
debranching enzyme
work?
7. What kind of linkage
con- nects the branch
points vs the
, BMB 402 Exam #2 Questions And Answers {100% correct}
Latest Update
Adds a phosphate to a nonreducing end
of a glycogen chain to make a glucose
1-phosphate
Using its transferase activity, the debranching enzyme
No, glycogen phosphorylase uses a free takes 3 of the 4 branched glucose residues and adds
inorganic phosphate them to the end of the adjacent strand. Next, using its
glucosidase activity, it removes the last glucose residue
Branch points: alpha1->6
Debranching enzyme unbranched polymer: alpha1->4
phosphoglucomutase
9.
, BMB 402 Exam #2 Questions And Answers {100% correct}
Latest Update
How does phosphogluco- 1. A serine residue adds a phosphate group to
mutase work? carbon 6 produc- ing a G16P intermediate
2. Same serine residue removes the phosphate
group from car- bon 1
There is an element of chance in this reaction; sometimes it goes
the right direction sometimes not. With enough
permutations, the reaction will eventually end up
producing the intended prod- uct
10. What are the main substrate Sugar nucleotides (UDP-glucose)
for the polymerization of
glucose into glycogen?
11. Why sugar nucleotides? 1. Formation is metabolically irreversible
2. The nucleotide moiety has many groups that can
participate in non-covalent interactions with enzymes
3. The nucleotidyl group is an excellent leaving group
4. Tagged hexoses with nucleotidyl groups can be set
aside by the cell for just one purpose
12. How are sugar NDP-sugar phosphorylase catalyzes the nucleophillic attack
nucleotides formed? of the negative charge from phosphate on a sugar
phosphate to the first phosphate atom in an NTP molecule
(small positive delta G)
13. If there is a small and
pos- itive delta G for The reaction also produces pyrophosphate (PPi) which is
the forma- con- verted to inorganic phosphate. This reaction has a
large negative
tion of the sugar nucleotide, delta G from the fast removal of the product
what drives the reaction
14. Which enzyme catalyzes pyrophos- phate to inorganic
the conversion of phos- phate?
, BMB 402 Exam #2 Questions And Answers {100% correct}
Latest Update
inorganic phosphatase
