BOC Immunology exam questions with answers

BOC Immunology exam questions with
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answers

1) Antinuclear antibody tests are performed to help diagnose?
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a) acute leukemia
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b) lupus erythematosus
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c) hemolytic anemia
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d) Crohn disease - CORRECT ANSWERS ✔✔b (lupus
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erythematosus)


2. In the anti-double-stranded DNA procedure, the antigen most
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commonly utilized is: |\ |\ |\




a. rat stomach tissue
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b. mouse kidney tissue
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c. Crithida lucilia
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d. Toxoplasma gondii - CORRECT ANSWERS ✔✔c (Crithida lucilia)
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3) Which of the ANA patterns shown above would be associated
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with high titers of antibodies to the Sm antigen?
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a) diagram A
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b) diagram B
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c) diagram C
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d) diagram D - CORRECT ANSWERS ✔✔c (diagram C)
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,4) Sera to be tested for IFA-ANA 6 days after drawing is best
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stored at? |\




a) room temperature
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b) 5°C +/- 2°
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c) -70°C in a constant temperature freezer
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d) -20°C in a frost-free self-defrosting freezer - CORRECT
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ANSWERS ✔✔b (5°C +/- 2°) |\ |\ |\ |\




5) Antibodies directed at native DNA are most frequently
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associated with which pattern of fluorescence in the IFA-ANA
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test?
a) rim
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b) diffuse
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c) speckled
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d) centromere - CORRECT ANSWERS ✔✔a (rim)
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6) The technologist observes apparent homogenous staining of
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the nucleus of interphase cells while performing an IFA-ANA, as
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well as staining of the chromosomes in mitotic cells. This result
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is:
a) indicative of 2 antibodies, which should be separately reported
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after titration
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b) expected for anti-DNA antibodies
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c) inconsistent; the test should be reported with new reagent
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d) expected for anti-centromere antibodies - CORRECT ANSWERS
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✔✔b (expected for anti-DNA antibodies)
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,7) The result of an anti-nuclear antibody test was a titer of 1:320
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with a peripheral pattern. Which of the following sets of results
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best correlate with these results?
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a) anti-dsDNA titer 1:80, and a high titer of antibodies to Sm
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b) antimitochondrial antibody titer 1:160, and antibodies to RNP
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c) anti-Scl-70, and antibodies to single-stranded DNA
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d) high titers of anti-SS-A and anti-SS-B - CORRECT ANSWERS
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✔✔a (anti-dsDNA titer 1:80, and a high titer of antibodies to Sm)
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8) Systemic lupus erythematosus patients often have which of
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the following test results?
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a) high titers of DNA antibody
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b) decreased serum immunoglobulin levels
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c) high titers of anti-smooth muscle antibodies
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d) high titers of antimitochondrial antibody - CORRECT ANSWERS
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✔✔a (high titers of DNA antibody)
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9. Systemic lupus erythematosus patients with active disease
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often have which of the following test results?
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a. high titers of antimicrosomal antibodies
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b. high titers of anti-smooth muscle antibodies
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c. marked decrease in serum CH50
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d. decreased serum immunoglobulin levels - CORRECT ANSWERS
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✔✔c (marked decrease in serum CH50)
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, 10. Which of the following is decreased in serum during the
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active stages of systemic lupus erythematosus?
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a. anti-nuclear antibody
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b. immune complexes
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c. complement (C3)
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d. anti-DNA - CORRECT ANSWERS ✔✔c (complement (C3))
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11. A positive ANA with the pattern of anticentromere antibodies
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is most frequently seen in patients with:
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a. rheumatoid arthritis
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b. systemic lupus erythematosus
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c. CREST syndrome
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d. Sjӧgren syndrome - CORRECT ANSWERS ✔✔c (CREST
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syndrome)


12. In the indirect fluorescent anti-nuclear antibody test, a
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homogenous pattern indicates the presence of antibody to: |\ |\ |\ |\ |\ |\ |\




a. RNP
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b. Sm
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c. RNA
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d. DNA - CORRECT ANSWERS ✔✔DNA
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