AGM 101
FUNDAMENTALS OF
MICROBIOLOGY (2+1)
Chapter 7 - Isolation, enrichment,
purification and Preservation
techniques of microorganisms
, Terminology/ Definition
Isolation
Isolation refers the separation of a microorganisms (strain)
from a natural, mixed population of living microbes, as
present in the environment.
Enumeration
Enumeration refers counting the total microbial cells present
in it.
Enrichment Cultures
Select for desired organisms through manipulation of
medium (nutrient content) and incubation conditions.
, Enumeration of Microorganisms from
nature
Microscopic count (Petroff-Hausser counting chamber)
• A total count of microbial numbers can be achieved using a
microscope to observe and enumerate the cells present in a culture
or natural sample. The method is simple, but the results can be
unreliable.
FUNDAMENTALS OF
MICROBIOLOGY (2+1)
Chapter 7 - Isolation, enrichment,
purification and Preservation
techniques of microorganisms
, Terminology/ Definition
Isolation
Isolation refers the separation of a microorganisms (strain)
from a natural, mixed population of living microbes, as
present in the environment.
Enumeration
Enumeration refers counting the total microbial cells present
in it.
Enrichment Cultures
Select for desired organisms through manipulation of
medium (nutrient content) and incubation conditions.
, Enumeration of Microorganisms from
nature
Microscopic count (Petroff-Hausser counting chamber)
• A total count of microbial numbers can be achieved using a
microscope to observe and enumerate the cells present in a culture
or natural sample. The method is simple, but the results can be
unreliable.
