ASCP MB Exam Preparation
ASCP MB EXAM PREPARATION FOR 2025/2026 COMPLETE 200
QUESTIONS AND CORRECT ANSWERS |ALREADY GRADED
A+||BRAND NEW!!
A patient with impaired judgment, personality changes, signs of abnormal body
movements and depression comes to the physician's office for a follow-up visit.
The physician suspects a single-gene disorder may be the cause of those clinical
manifestations. A blood specimen was then sent to your clinical laboratory for
mutation screening in the Huntingtin gene. Testing with standard PCR indicates
that patient has Huntington Disease, HD. Which of the following would be
consistent with this diagnosis?
25 CAG repeats in the Huntingtin gene
85 CAG repeats in the Huntingtin gene
25 CGA repeats in the Huntingtin gene
85 CGA repeats in the Huntingtin gene
85 CAG repeats in the Huntingtin gene
Which of the following is not a PCR step?
Denaturation
Elongation
Annealing
Termination
Termination
You could use all of the following methods to test for the t(9;22) translocation,
except:
FISH
1|Page
, ASCP MB Exam Preparation
Southern blot
Reverse-transcriptase PCR
Ribotyping
Ribotyping
What enzyme is crucial in the making RNA from DNA?
DNA Polymerase
RNA Polymerase
Helicase
Primase
RNA Polymerase
Splicing is the process that does which of the following?
Remove exons and preserve introns?
Remove introns and preserve exons?
Remove mutated regions of primary transcript RNA (tRNA)?
Add a poly-A tail to the end of a primary RNA transcript?
Remove introns and preserve exons
Deletion in the paternal chromosome 15: del(15)(q11q13) results in Prader-willi
syndrome. However, deletion in the same region in the maternal chromosome
results in a completely different condition known as Angelman syndrome. This
phenomenon is an example of:
Mosaicism
Loss of heterozygosity
Hemizygosity
Genomic imprinting
2|Page
, ASCP MB Exam Preparation
Genomic imprinting
All of the following are liquid tumors, except:
Mantle cell lymphoma
Ewing sarcoma
Burkitt’s lymphoma
Acute promyelocytic leukemia
Ewing sarcoma
Clinical laboratories must have clearly written protocols in place describing
handling of specimens for clinical testing. Many factors can affect testing
performance before the actual testing is conducted. Such factors or variables are
collectively known as pre-analytical. From the list below, select all the pre-
analytical variables that can have a negative impact on clinical testing (Hint: more
than one answer choice!)
Storage conditions
Patient identifiers
Anticoagulant in collection tubes
Transport procedures
Introduction of Heparin in all blood tubes to maintain specimen integrity for
testing
Thermo cycling parameters
DNA isolation method used
Testing analysis software used
Storage conditions
Patient identifiers
3|Page
, ASCP MB Exam Preparation
Anticoagulant in collection tubes
Transport procedures
Imagine you are working in specimen processing for the molecular laboratory and
you receive a patient’s specimen collected in a lavender top vacutainer tube
containing EDTA. The test that is ordered requires that the specimen be collected
in a yellow top ACD vacutainer tube. What is the BEST action to take in this
instance?
Reject the specimen, inform the supervisor and request a new specimen drawn in
ACD
Process the sample according to laboratory protocol because EDTA does not
interfere with molecular testing methods
Document the tube additive change, process the sample, run the test, and hold
the results until a supervisor or laboratory director signs off
Follow the prescribed laboratory protocol for accepting and rejecting specimens
Both B & D are correct
Both C & D are correct
Follow the prescribed laboratory protocol for accepting and rejecting specimens
Which one of the following complementary pairings is incorrect?
A=T
G=C
U=T
A=U
U=T
Purines and pyrimidines differ from each other in that:
Purines are found RNA; pyrimidines are found in DNA
4|Page
ASCP MB EXAM PREPARATION FOR 2025/2026 COMPLETE 200
QUESTIONS AND CORRECT ANSWERS |ALREADY GRADED
A+||BRAND NEW!!
A patient with impaired judgment, personality changes, signs of abnormal body
movements and depression comes to the physician's office for a follow-up visit.
The physician suspects a single-gene disorder may be the cause of those clinical
manifestations. A blood specimen was then sent to your clinical laboratory for
mutation screening in the Huntingtin gene. Testing with standard PCR indicates
that patient has Huntington Disease, HD. Which of the following would be
consistent with this diagnosis?
25 CAG repeats in the Huntingtin gene
85 CAG repeats in the Huntingtin gene
25 CGA repeats in the Huntingtin gene
85 CGA repeats in the Huntingtin gene
85 CAG repeats in the Huntingtin gene
Which of the following is not a PCR step?
Denaturation
Elongation
Annealing
Termination
Termination
You could use all of the following methods to test for the t(9;22) translocation,
except:
FISH
1|Page
, ASCP MB Exam Preparation
Southern blot
Reverse-transcriptase PCR
Ribotyping
Ribotyping
What enzyme is crucial in the making RNA from DNA?
DNA Polymerase
RNA Polymerase
Helicase
Primase
RNA Polymerase
Splicing is the process that does which of the following?
Remove exons and preserve introns?
Remove introns and preserve exons?
Remove mutated regions of primary transcript RNA (tRNA)?
Add a poly-A tail to the end of a primary RNA transcript?
Remove introns and preserve exons
Deletion in the paternal chromosome 15: del(15)(q11q13) results in Prader-willi
syndrome. However, deletion in the same region in the maternal chromosome
results in a completely different condition known as Angelman syndrome. This
phenomenon is an example of:
Mosaicism
Loss of heterozygosity
Hemizygosity
Genomic imprinting
2|Page
, ASCP MB Exam Preparation
Genomic imprinting
All of the following are liquid tumors, except:
Mantle cell lymphoma
Ewing sarcoma
Burkitt’s lymphoma
Acute promyelocytic leukemia
Ewing sarcoma
Clinical laboratories must have clearly written protocols in place describing
handling of specimens for clinical testing. Many factors can affect testing
performance before the actual testing is conducted. Such factors or variables are
collectively known as pre-analytical. From the list below, select all the pre-
analytical variables that can have a negative impact on clinical testing (Hint: more
than one answer choice!)
Storage conditions
Patient identifiers
Anticoagulant in collection tubes
Transport procedures
Introduction of Heparin in all blood tubes to maintain specimen integrity for
testing
Thermo cycling parameters
DNA isolation method used
Testing analysis software used
Storage conditions
Patient identifiers
3|Page
, ASCP MB Exam Preparation
Anticoagulant in collection tubes
Transport procedures
Imagine you are working in specimen processing for the molecular laboratory and
you receive a patient’s specimen collected in a lavender top vacutainer tube
containing EDTA. The test that is ordered requires that the specimen be collected
in a yellow top ACD vacutainer tube. What is the BEST action to take in this
instance?
Reject the specimen, inform the supervisor and request a new specimen drawn in
ACD
Process the sample according to laboratory protocol because EDTA does not
interfere with molecular testing methods
Document the tube additive change, process the sample, run the test, and hold
the results until a supervisor or laboratory director signs off
Follow the prescribed laboratory protocol for accepting and rejecting specimens
Both B & D are correct
Both C & D are correct
Follow the prescribed laboratory protocol for accepting and rejecting specimens
Which one of the following complementary pairings is incorrect?
A=T
G=C
U=T
A=U
U=T
Purines and pyrimidines differ from each other in that:
Purines are found RNA; pyrimidines are found in DNA
4|Page
