Tools of Microbiology (Microbiology: A Systems Approach 7th ed.)
Study online at https://quizlet.com/_hhqaqn
1. Inoculation five I's
Incubation
Isolation
Inspection
Identification
2. aseptic technique and sterile principles to consider before handling microorganisms
equipment
3. Inoculation Introducing specimen to culture medium
4. Culture propagation of microorganisms with various media
5. Medium A nutrient-containing environment in which microbes can multiply
6. Sterile free of all life forms and viruses and prions
7. Incubation maintaining specific conditions optimal for microorganisms
8. cloudiness (turbidity), sedi- Microbial growth in liquid media can be seen as
ment, scum, color
9. colonies Microbial growth on solid medium grow as
10. Robert Koch Father of culture media
11. Robert Koch developed meat extract with gelatin in glass slides
12. Fannie Eilsthemius Suggested to replace gelatin with agar
13. Julius Richard Petri produced the Petri dish
14. Overhanging lid key design feature of the Petri dish
15. Edwin Klebs
1/7
, Tools of Microbiology (Microbiology: A Systems Approach 7th ed.)
Study online at https://quizlet.com/_hhqaqn
observed bacteria that grow well in culture media containing
"peptones"
16. Friedrich Loeffler added salt and peptones to Koch's basic meat extract formulation
which raised osmolarity of culture media
17. oxygen and carbon dioxide atmospheric gases controlled by incubators
18. 20-45°C usual temperatures used in lab
19. 25-30°C and 1-2 days Optimum growth temperature (°C) and Culturing time (days) of
Listeria monocytogenes and Pseudomonas fluorescens
20. 37°C and 1-2 days Optimum growth temperature (°C) and Culturing time (days) of
Streptococcus pyogenes
21. 37°C and 28 days Optimum growth temperature (°C) and Culturing time (days) of
Mycobacterium tuberculosis
22. 27-30°C and 14 days in vivo Optimum growth temperature (°C) and Culturing time (days) of
Mycobacterium leprae
23. Physical state, Chemi- classifications of media according to three properties
cal composition, Functional
type (purpose)
24. brain-heart infusion an example of general purpose and enriched artificial media
25. selective and differential function/s of mannitol salt agar as an artificial media
26. Selective and differential function/s of MacConkey agar as an artificial media
27. enriched and differential function/s of blood agar as an artificial media
28. liquid media Broths, milks, and infusions are all examples of
2/7
Study online at https://quizlet.com/_hhqaqn
1. Inoculation five I's
Incubation
Isolation
Inspection
Identification
2. aseptic technique and sterile principles to consider before handling microorganisms
equipment
3. Inoculation Introducing specimen to culture medium
4. Culture propagation of microorganisms with various media
5. Medium A nutrient-containing environment in which microbes can multiply
6. Sterile free of all life forms and viruses and prions
7. Incubation maintaining specific conditions optimal for microorganisms
8. cloudiness (turbidity), sedi- Microbial growth in liquid media can be seen as
ment, scum, color
9. colonies Microbial growth on solid medium grow as
10. Robert Koch Father of culture media
11. Robert Koch developed meat extract with gelatin in glass slides
12. Fannie Eilsthemius Suggested to replace gelatin with agar
13. Julius Richard Petri produced the Petri dish
14. Overhanging lid key design feature of the Petri dish
15. Edwin Klebs
1/7
, Tools of Microbiology (Microbiology: A Systems Approach 7th ed.)
Study online at https://quizlet.com/_hhqaqn
observed bacteria that grow well in culture media containing
"peptones"
16. Friedrich Loeffler added salt and peptones to Koch's basic meat extract formulation
which raised osmolarity of culture media
17. oxygen and carbon dioxide atmospheric gases controlled by incubators
18. 20-45°C usual temperatures used in lab
19. 25-30°C and 1-2 days Optimum growth temperature (°C) and Culturing time (days) of
Listeria monocytogenes and Pseudomonas fluorescens
20. 37°C and 1-2 days Optimum growth temperature (°C) and Culturing time (days) of
Streptococcus pyogenes
21. 37°C and 28 days Optimum growth temperature (°C) and Culturing time (days) of
Mycobacterium tuberculosis
22. 27-30°C and 14 days in vivo Optimum growth temperature (°C) and Culturing time (days) of
Mycobacterium leprae
23. Physical state, Chemi- classifications of media according to three properties
cal composition, Functional
type (purpose)
24. brain-heart infusion an example of general purpose and enriched artificial media
25. selective and differential function/s of mannitol salt agar as an artificial media
26. Selective and differential function/s of MacConkey agar as an artificial media
27. enriched and differential function/s of blood agar as an artificial media
28. liquid media Broths, milks, and infusions are all examples of
2/7
