As level
BIOLOGY
, structure of a eukaryotic cell
under a light microscope a nucleus , large vacuole , cell surface membrane, cytoplasm sometimes mitochondria /chloroplasts
·
,
,
Def-organelles are part of a cell that has a specific function
viewing cells under an optical Microscope
·
light microscopes work by passing light upwards through a very thin specimen Uses light
.
rays so it has a large wh+ low resolution
.
Wet Mount
·
specimen suspended in liquid (water/immersion oil) + coverslip placed at an angle
.
Squash slide
prepare a wet slide + use a lens tissue to gently press down on coverslip to ensure sample is I cell thick + can be seen with better
·
Clarity .
smear slide
·
edge of slide used to smear the sample by creating a thin even coating on another slide
.
Temporary mount
① Add drop of water on microscopic slide -
prevents dessication of specimen underlight
②obtain a thin section of specimen - to obtain a single layer of specimen so light can pass through
⑤ Add drop of stain -
make organelles more visible , to contrast between structures
① Lower coverslip using mounted needle--to avoid bubbles appearing between coverslip + specimen
Optical vs Election Microscope.
Def- Resolution is the ability to separate out objects that are close together + see greater detail
Light Microscopes
·
uses light rays , have longer who radiation medium-air- can only see large organelles can only distinguish between objects If 200 nm
apart
·
Magnification up to X1500 low resolution (Max o .
2 mm) ·
2D image
.
. Temporary mount
specimen prep Thin section stained :
V can view live specimens X low resolution + magnification X only whole cells large organelles
, can be
V can study whole cells + larger organelles X cannot see greater detail of organelles seen
.
Electron Microscopes
·
uses electrons + have shorter we can distinguish between small objects that are close up to 0 Inm can see smaller organelles .
.
·
·
higher resolution
·
can see smaller organelles
BIOLOGY
, structure of a eukaryotic cell
under a light microscope a nucleus , large vacuole , cell surface membrane, cytoplasm sometimes mitochondria /chloroplasts
·
,
,
Def-organelles are part of a cell that has a specific function
viewing cells under an optical Microscope
·
light microscopes work by passing light upwards through a very thin specimen Uses light
.
rays so it has a large wh+ low resolution
.
Wet Mount
·
specimen suspended in liquid (water/immersion oil) + coverslip placed at an angle
.
Squash slide
prepare a wet slide + use a lens tissue to gently press down on coverslip to ensure sample is I cell thick + can be seen with better
·
Clarity .
smear slide
·
edge of slide used to smear the sample by creating a thin even coating on another slide
.
Temporary mount
① Add drop of water on microscopic slide -
prevents dessication of specimen underlight
②obtain a thin section of specimen - to obtain a single layer of specimen so light can pass through
⑤ Add drop of stain -
make organelles more visible , to contrast between structures
① Lower coverslip using mounted needle--to avoid bubbles appearing between coverslip + specimen
Optical vs Election Microscope.
Def- Resolution is the ability to separate out objects that are close together + see greater detail
Light Microscopes
·
uses light rays , have longer who radiation medium-air- can only see large organelles can only distinguish between objects If 200 nm
apart
·
Magnification up to X1500 low resolution (Max o .
2 mm) ·
2D image
.
. Temporary mount
specimen prep Thin section stained :
V can view live specimens X low resolution + magnification X only whole cells large organelles
, can be
V can study whole cells + larger organelles X cannot see greater detail of organelles seen
.
Electron Microscopes
·
uses electrons + have shorter we can distinguish between small objects that are close up to 0 Inm can see smaller organelles .
.
·
·
higher resolution
·
can see smaller organelles
