BIMM 100 UCSD questions and
answers graded A+
Phenol - correct answer ✔✔ Removes protein in heat killed bacteria
UV light - correct answer ✔✔ Removes DNA & RNA in heat killed bacteria
Adenine - correct answer ✔✔
Guanine - correct answer ✔✔
Uracil - correct answer ✔✔
Thymine - correct answer ✔✔
Cytosine - correct answer ✔✔
Only fraction of DNA transcribed & some RNA accumulate at much higher levels than others -
correct answer ✔✔ Why ratio of bases in RNA different than in DNA
Primer, dNTP, DNA template - correct answer ✔✔ DNA polymerase needs
Primase, DNA Polymerase, Nuclease, DNA Polymerase, DNA Ligase - correct answer ✔✔
Enzymes in replication
, Telomeres - correct answer ✔✔ A compound structure at the end of chromosomes consisting of
1000's of sequence repeats
Reverse transcriptase - correct answer ✔✔ What type of enzyme is Telomerase?
Origin of replication, 2 telomeres & centromere - correct answer ✔✔ What is needed to make
an artificial chromosome?
DNA polymerase, 3' to 5' exonuclease activity - correct answer ✔✔ Proofreading by which
enzyme and in what direction?
Exonuclease - correct answer ✔✔ Type of activity that cleaves the nucleotides at the ends of
the DNA molecule
Endonuclease - correct answer ✔✔ Type of activity that cleaves the nucleotides in the middle of
the DNA molecule
Mismatch excision repair - correct answer ✔✔ Type of repair for incorrect nucleotide pairing
not proofread, after replication
Base excision repair - correct answer ✔✔ Type of repair for chemically changed bases
(deamination), after replication
Nucleotide excision repair - correct answer ✔✔ Type of repair for distorted DNA (UV irradiation)
after replication
Bypass synthesis - correct answer ✔✔ When repair machinery doesn't know what to do
1. Break recognized & exonucleases remove NTPs leaving 3' end ssDNA overhangs
answers graded A+
Phenol - correct answer ✔✔ Removes protein in heat killed bacteria
UV light - correct answer ✔✔ Removes DNA & RNA in heat killed bacteria
Adenine - correct answer ✔✔
Guanine - correct answer ✔✔
Uracil - correct answer ✔✔
Thymine - correct answer ✔✔
Cytosine - correct answer ✔✔
Only fraction of DNA transcribed & some RNA accumulate at much higher levels than others -
correct answer ✔✔ Why ratio of bases in RNA different than in DNA
Primer, dNTP, DNA template - correct answer ✔✔ DNA polymerase needs
Primase, DNA Polymerase, Nuclease, DNA Polymerase, DNA Ligase - correct answer ✔✔
Enzymes in replication
, Telomeres - correct answer ✔✔ A compound structure at the end of chromosomes consisting of
1000's of sequence repeats
Reverse transcriptase - correct answer ✔✔ What type of enzyme is Telomerase?
Origin of replication, 2 telomeres & centromere - correct answer ✔✔ What is needed to make
an artificial chromosome?
DNA polymerase, 3' to 5' exonuclease activity - correct answer ✔✔ Proofreading by which
enzyme and in what direction?
Exonuclease - correct answer ✔✔ Type of activity that cleaves the nucleotides at the ends of
the DNA molecule
Endonuclease - correct answer ✔✔ Type of activity that cleaves the nucleotides in the middle of
the DNA molecule
Mismatch excision repair - correct answer ✔✔ Type of repair for incorrect nucleotide pairing
not proofread, after replication
Base excision repair - correct answer ✔✔ Type of repair for chemically changed bases
(deamination), after replication
Nucleotide excision repair - correct answer ✔✔ Type of repair for distorted DNA (UV irradiation)
after replication
Bypass synthesis - correct answer ✔✔ When repair machinery doesn't know what to do
1. Break recognized & exonucleases remove NTPs leaving 3' end ssDNA overhangs
