BIMM 100 Midterm 2 questions well
answered rated A+
transposase - correct answer ✔✔ enzyme which recognizes host DNA and cuts and glues
transposons in and out of genome
Transposons - correct answer ✔✔ (jumping genes) short strands of DNA capable of moving
from one location to another within a cell's genetic material
reverse transcriptase - correct answer ✔✔ a polymerase that catalyzes the formation of DNA
using RNA as a template
Retrotransposons - correct answer ✔✔ Transposable elements that move within a genome by
means of an RNA intermediate, a transcript of the retrotransposon DNA. (copy & paste)
DNA transposons - correct answer ✔✔ "jumping genes" remove themselves from one place in
the genome and move to a new site (cut & paste)
Promoter - correct answer ✔✔ A specific nucleotide sequence in DNA that binds RNA
polymerase and indicates where to start transcribing RNA.
5'UTR - correct answer ✔✔ (Untranslated region) a region of mRNA upstream of the start codon
that is not translated
coding region - correct answer ✔✔ Portion of the gene that codes for the amino acid sequence
of a protein.
, 3'UTR - correct answer ✔✔ (Untranslated region) a region of mRNA downstream of the stop
codon that is not translated
RNase - correct answer ✔✔ degrades RNA
sigma factor - correct answer ✔✔ protein required by prokaryotic RNA polymerases to initiate
transcription at the promoter region of DNA
RNA polymerase - correct answer ✔✔ enzyme that links together the growing chain of RNA
nucleotides during transcription using a DNA strand as a template
transcription factors - correct answer ✔✔ Proteins that assist polymerase binding at
transcription start sites
TATA box - correct answer ✔✔ A promoter DNA sequence crucial in forming the transcription
initiation complex.
TATA box binding protein (TBP) - correct answer ✔✔ eukaryotic transcription factor which binds
to the TATA box and causes a writhe (3D bend) in the DNA, allowing for RNA pol. to reach the
promoter region (since RNA pol. cannot recognize promoter) Recruits RNA Pol I to rRNA
promoters and assists Pol II to bind to coding gene trxn start sites.
TBP-associated factors (TAFs) - correct answer ✔✔ polypeptide that associates with TBP to form
the TFIID complex and includes proteins that are required for activation of transcription
TFIID - correct answer ✔✔ the first general transcription factor to bind the promoter, binds to
the TATA box through the TATA binding protein (TBP). Recruits RNA Polymerase II, Recruits trxn
factor 2h for phosphorylating CTD of RNA polymerase II to start trxn.
EMSA - correct answer ✔✔ Electrophoretic mobility shift assay
answered rated A+
transposase - correct answer ✔✔ enzyme which recognizes host DNA and cuts and glues
transposons in and out of genome
Transposons - correct answer ✔✔ (jumping genes) short strands of DNA capable of moving
from one location to another within a cell's genetic material
reverse transcriptase - correct answer ✔✔ a polymerase that catalyzes the formation of DNA
using RNA as a template
Retrotransposons - correct answer ✔✔ Transposable elements that move within a genome by
means of an RNA intermediate, a transcript of the retrotransposon DNA. (copy & paste)
DNA transposons - correct answer ✔✔ "jumping genes" remove themselves from one place in
the genome and move to a new site (cut & paste)
Promoter - correct answer ✔✔ A specific nucleotide sequence in DNA that binds RNA
polymerase and indicates where to start transcribing RNA.
5'UTR - correct answer ✔✔ (Untranslated region) a region of mRNA upstream of the start codon
that is not translated
coding region - correct answer ✔✔ Portion of the gene that codes for the amino acid sequence
of a protein.
, 3'UTR - correct answer ✔✔ (Untranslated region) a region of mRNA downstream of the stop
codon that is not translated
RNase - correct answer ✔✔ degrades RNA
sigma factor - correct answer ✔✔ protein required by prokaryotic RNA polymerases to initiate
transcription at the promoter region of DNA
RNA polymerase - correct answer ✔✔ enzyme that links together the growing chain of RNA
nucleotides during transcription using a DNA strand as a template
transcription factors - correct answer ✔✔ Proteins that assist polymerase binding at
transcription start sites
TATA box - correct answer ✔✔ A promoter DNA sequence crucial in forming the transcription
initiation complex.
TATA box binding protein (TBP) - correct answer ✔✔ eukaryotic transcription factor which binds
to the TATA box and causes a writhe (3D bend) in the DNA, allowing for RNA pol. to reach the
promoter region (since RNA pol. cannot recognize promoter) Recruits RNA Pol I to rRNA
promoters and assists Pol II to bind to coding gene trxn start sites.
TBP-associated factors (TAFs) - correct answer ✔✔ polypeptide that associates with TBP to form
the TFIID complex and includes proteins that are required for activation of transcription
TFIID - correct answer ✔✔ the first general transcription factor to bind the promoter, binds to
the TATA box through the TATA binding protein (TBP). Recruits RNA Polymerase II, Recruits trxn
factor 2h for phosphorylating CTD of RNA polymerase II to start trxn.
EMSA - correct answer ✔✔ Electrophoretic mobility shift assay
