ULTIMATE CBSPD CERTIFICATION STUDY GUIDE 2025-2026,
100 QUESTIONS WITH ANSWERS & KEY REFERENCES,100%
CORRECT ALREADY GRADED A+
1. Decontamination – Water Temperature
Question: What is the recommended water temperature for manual cleaning
of surgical instruments?
A) Below 60°F (16°C)
B) 80–110°F (27–43°C)
C) Above 140°F (60°C)
D) Room temperature only
Correct Answer: B
Rationale: Warm water (80–110°F) enhances detergent action and soil
removal without coagulating protein.
Reference: AAMI ST79 Standards
2. Decontamination – PPE
Question: Which PPE is required in the decontamination area?
A) Surgical mask only
B) Gloves, gown, mask/face shield, and eye protection
C) Hair cover only
D) Shoe covers only
Correct Answer: B
Rationale: Full PPE prevents exposure to bloodborne pathogens and
chemical hazards.
Reference: OSHA Bloodborne Pathogens Standard
3. Cleaning – Instrument Lubrication
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Question: Instrument lubrication is applied:
A) Before cleaning
B) After final rinse, before sterilization
C) During sterilization
D) Never
Correct Answer: B
Rationale: Lubrication maintains function and prevents corrosion; it must
be water-soluble and steam-permeable.
Reference: [AAMI ST79]
4. Cleaning – Automated Washer
Question: The purpose of the final rinse in an automated washer is:
A) Remove detergent only
B) Cool instruments
C) Thermal disinfection
D) Add lubricant
Correct Answer: C
Rationale: Final rinse uses elevated temperature water to achieve
disinfection and reduce bioburden.
Reference: [AAMI ST79]
5. Sterilization – Steam Cycle
Question: Standard pre-vacuum steam sterilization cycle parameters are:
A) 121°C for 15 minutes
B) 132°C for 10 minutes
C) 132°C (270°F) for 4 minutes at 27–30 psi
D) 140°C for 3 minutes
Correct Answer: C
Rationale: Pre-vacuum cycles allow shorter exposure due to air removal
and steam penetration.
Reference: [AAMI ST79]
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6. Sterilization – BI
Question: Which biological indicator organism is used for steam
sterilization monitoring?
A) Bacillus subtilis
B) Geobacillus stearothermophilus
C) Bacillus atrophaeus
D) Clostridium perfringens
Correct Answer: B
Rationale: G. stearothermophilus spores are highly resistant to moist heat,
making them ideal for monitoring.
Reference: CDC Sterilization Monitoring
7. Sterilization – EO Gas
Question: Which biological indicator is used for EO gas sterilization?
A) Bacillus atrophaeus
B) Geobacillus stearothermophilus
C) Candida albicans
D) Clostridium difficile
Correct Answer: A
Rationale: B. atrophaeus spores are resistant to EO gas and are the industry
standard for BI testing.
Reference: [AAMI ST41]
8. Packaging – Peel Pouch
Question: When packaging items in peel pouches, instruments should be:
A) Open and locked
B) Open and unlocked
C) Closed tightly
D) Double-packed with cotton
Correct Answer: B
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Rationale: Open instruments allow steam or gas to contact all surfaces
during sterilization.
Reference: [AAMI ST79]
9. Packaging – Count Sheet
Question: Where should the count sheet be placed in a wrapped set?
A) Outside the wrap
B) Inside the set, on top, in a way that does not block steam flow
C) Under the bottom tray
D) Taped to the outside of the tray
Correct Answer: B
Rationale: Proper placement allows staff to verify contents without
compromising sterilant penetration.
Reference: [AAMI ST79]
10. Storage – Event-Related Sterility
Question: Shelf life of a sterile package is based on:
A) Fixed number of days
B) Event-related sterility
C) Expiration date on tape only
D) Calendar month
Correct Answer: B
Rationale: Packages remain sterile until compromised by an event (damage,
moisture, seal failure).
Reference: [CDC Guidelines]
11. Infection Control – Spaulding Classification
Question: Spaulding’s classification system is used to:
A) Determine surgical site prep
B) Classify medical devices by required disinfection/sterilization level