UArk Microbiology Lab Practical Exam
2026 Questions and Answers
Flagella - Correct answer-Major organelle for cell motility, consisting of a helical
structure that is connected to a "basal body" by a "hook"
What is the primary stain for gram+ and gram- cells? - Correct answer-crystal
violet
-> grams iodine -> decolorization (acetone) -> safranin (gram neg will be pink)
Contents of the Flagella Basal Body - Correct answer-An M-Ring and an S-Ring
Gram Negative have an additional L and P ring
Wet mount - Correct answer-Used to observe the motility of a cell using a phase
contrast microscope
Brownian Motion - Correct answer-Movement of cells caused by currents in a wet
mount
Pure Cultures - Correct answer-contains only a single kind of an organism
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, Two common procedures used to obtain a pure colony - Correct answer-pour and
streak plates and quadrant streak
What acid is used in acid fast staining? - Correct answer-acid alcohol and stained
with methylene blue
What color is acid fast and non acid fast - Correct answer-acid fast-pink
non acid fast- blue
Bacteriophages - Correct answer-viruses that infect bacterial cells
Obligate Intracellular Parasites - Correct answer-must invade a host cell in order to
replicate and reproduce
Purpose of FTM Test - Correct answer-To determine oxygen requirements of a
bacteria
Bacteria growth on top = Aerobic
Growth on bottom = Anaerobic
Growth on both = Faculative anaerobe
Purpose of Gelatin Stab - Correct answer-To determine if unknown bacteria
produces gelatinase/proteases
Liquefaction of gelatin = positive
©COPYRIGHT 2025, ALL RIGHTS RESERVE 2
2026 Questions and Answers
Flagella - Correct answer-Major organelle for cell motility, consisting of a helical
structure that is connected to a "basal body" by a "hook"
What is the primary stain for gram+ and gram- cells? - Correct answer-crystal
violet
-> grams iodine -> decolorization (acetone) -> safranin (gram neg will be pink)
Contents of the Flagella Basal Body - Correct answer-An M-Ring and an S-Ring
Gram Negative have an additional L and P ring
Wet mount - Correct answer-Used to observe the motility of a cell using a phase
contrast microscope
Brownian Motion - Correct answer-Movement of cells caused by currents in a wet
mount
Pure Cultures - Correct answer-contains only a single kind of an organism
©COPYRIGHT 2025, ALL RIGHTS RESERVE 1
, Two common procedures used to obtain a pure colony - Correct answer-pour and
streak plates and quadrant streak
What acid is used in acid fast staining? - Correct answer-acid alcohol and stained
with methylene blue
What color is acid fast and non acid fast - Correct answer-acid fast-pink
non acid fast- blue
Bacteriophages - Correct answer-viruses that infect bacterial cells
Obligate Intracellular Parasites - Correct answer-must invade a host cell in order to
replicate and reproduce
Purpose of FTM Test - Correct answer-To determine oxygen requirements of a
bacteria
Bacteria growth on top = Aerobic
Growth on bottom = Anaerobic
Growth on both = Faculative anaerobe
Purpose of Gelatin Stab - Correct answer-To determine if unknown bacteria
produces gelatinase/proteases
Liquefaction of gelatin = positive
©COPYRIGHT 2025, ALL RIGHTS RESERVE 2
