BIMM 101 Quiz 2 questions and
answers rated A+
EDTA - correct answer ✔✔ P1: Chelates Mg2+ which is part of the cell wall= destabilizes cell wall
RNAse A - correct answer ✔✔ P1: Degrades cellular RNA
SDS - correct answer ✔✔ P2: Detergent= dissolves lipids (plasma membrane, denatured
proteins
NaOH - correct answer ✔✔ P2: Raises pH, degrades RNA, denatures chromosomal and plasmid
DNA
Potassium acetate - correct answer ✔✔ N3: Neutralizes pH to renature plasmid DNA; The
potassium and SDS precipitates with the cellular debris
Silica Column - correct answer ✔✔ Traps DNA at a high salt condition and lets us remove DNA
from supernatant after cell lysis and centrifugation
Ethanol/Isopropanol - correct answer ✔✔ Wash: Washes excess salt and other material off
column
Tris-Cl - correct answer ✔✔ Elution Buffer: Slightly alkaline pH dissolves DNA to allow plasmid to
be removed from column
Materials for PCR - correct answer ✔✔ Template DNA
DNA primers
answers rated A+
EDTA - correct answer ✔✔ P1: Chelates Mg2+ which is part of the cell wall= destabilizes cell wall
RNAse A - correct answer ✔✔ P1: Degrades cellular RNA
SDS - correct answer ✔✔ P2: Detergent= dissolves lipids (plasma membrane, denatured
proteins
NaOH - correct answer ✔✔ P2: Raises pH, degrades RNA, denatures chromosomal and plasmid
DNA
Potassium acetate - correct answer ✔✔ N3: Neutralizes pH to renature plasmid DNA; The
potassium and SDS precipitates with the cellular debris
Silica Column - correct answer ✔✔ Traps DNA at a high salt condition and lets us remove DNA
from supernatant after cell lysis and centrifugation
Ethanol/Isopropanol - correct answer ✔✔ Wash: Washes excess salt and other material off
column
Tris-Cl - correct answer ✔✔ Elution Buffer: Slightly alkaline pH dissolves DNA to allow plasmid to
be removed from column
Materials for PCR - correct answer ✔✔ Template DNA
DNA primers
