BIO 200 EXAM 2 LABS QUESTIONS
AND ANSWERS
What molecule is the source of information for transcription? - Correct Answers -DNA-
The nucleotide sequence of DNA is used by RNA polymerase to guide the construction
of RNA molecules.
What is the main enzyme for making a polymer of RNA? - Correct Answers -RNA
Polymerase- RNA polymerase catalyzes phosphodiester bond formation. The order of
the RNA is based on base-pairing between the DNA template and the RNA nucleotides.
Where does transcription occur in a eukaryotic cell? - Correct Answers -Nucleus-
Eukaryotic DNA is kept safely locked in the membrane-bound organelle known as the
nucleus. To perform transcription, the DNA template must be directly contacted.
Where does transcription occur in a prokaryotic cell? - Correct Answers -Cytoplasm-
Prokaryotic cells lack membrane-bound organelles, so both transcription and translation
must occur in the same area within the cell known (the cytoplasm).
Initiation of transcription at the correct location starts when sigma binds at the -10 and -
35 sequences of the DNA. The core RNA polymerase enzyme then binds to the DNA
and starts to separate the DNA strands at the promoter in order to create a new strand
based on RNA-DNA basepairing.
Which of these choices is the general outline for the Central Dogma Workshop
exercises? - Correct Answers -DNA structure, Transcription, Translation, Genetic
Diseases
Why are some genes constitutively expressed while the expression of others is
regulated? - Correct Answers -Some gene products are needed at all times, and other
gene products are only produced under some conditions to avoid waste and respond to
stimuli.
Where does translation occur in a eukaryotic cell? - Correct Answers -Cytoplasm-
Ribosomes are located outside of the nucleus, so mRNA molecules are brought outside
of the nucleus into the cytoplasm to complete translation.
AND ANSWERS
What molecule is the source of information for transcription? - Correct Answers -DNA-
The nucleotide sequence of DNA is used by RNA polymerase to guide the construction
of RNA molecules.
What is the main enzyme for making a polymer of RNA? - Correct Answers -RNA
Polymerase- RNA polymerase catalyzes phosphodiester bond formation. The order of
the RNA is based on base-pairing between the DNA template and the RNA nucleotides.
Where does transcription occur in a eukaryotic cell? - Correct Answers -Nucleus-
Eukaryotic DNA is kept safely locked in the membrane-bound organelle known as the
nucleus. To perform transcription, the DNA template must be directly contacted.
Where does transcription occur in a prokaryotic cell? - Correct Answers -Cytoplasm-
Prokaryotic cells lack membrane-bound organelles, so both transcription and translation
must occur in the same area within the cell known (the cytoplasm).
Initiation of transcription at the correct location starts when sigma binds at the -10 and -
35 sequences of the DNA. The core RNA polymerase enzyme then binds to the DNA
and starts to separate the DNA strands at the promoter in order to create a new strand
based on RNA-DNA basepairing.
Which of these choices is the general outline for the Central Dogma Workshop
exercises? - Correct Answers -DNA structure, Transcription, Translation, Genetic
Diseases
Why are some genes constitutively expressed while the expression of others is
regulated? - Correct Answers -Some gene products are needed at all times, and other
gene products are only produced under some conditions to avoid waste and respond to
stimuli.
Where does translation occur in a eukaryotic cell? - Correct Answers -Cytoplasm-
Ribosomes are located outside of the nucleus, so mRNA molecules are brought outside
of the nucleus into the cytoplasm to complete translation.
