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, Laborator
Anatomy and Physiology Laboratory Safety
*
Guidelines
1. Upon entering the laboratory, locate exits, fire extinguisher, fire blanket,
chemical shower, eyewash station, first aid kit, containers for broken glass,
and materials for cleaning up spills.
2. Do not eat, drink, smoke, handle contact lenses, store food, or apply
cosmetics or lip balm in the laboratory. Restrain long hair, loose clothing,
and dangling jewelry.
3. Students who are pregnant, are taking immunosuppressive drugs, or have any
other medical conditions (e.g., diabetes, immunological defect) that might
necessitate special precautions in the laboratory must inform the instructor
immediately.
4. Wearing contact lenses in the laboratory is inadvisable because they do not
provide eye protection and may trap material on the surface of the eye. Soft
contact lenses may absorb volatile chemicals. If possible, wear regular
eyeglasses instead.
5. Use safety glasses in all experiments involving liquids, aerosols, vapors, and
gases.
6. Decontaminate work surfaces at the beginning and end of every lab
period, using a commercially prepared disinfectant or 10% bleach solution.
After labs involving dissection of preserved material, use hot soapy water
or disinfectant.
7. Keep all liquids away from the edge of the lab bench to avoid spills. Clean
up spills of viable materials using disinfectant or 10% bleach solution.
8. Properly label glassware and slides.
9. Use mechanical pipetting devices; mouth pipetting is prohibited.
10. Wear disposable gloves when handling blood and other body fluids,
mucous membranes, and nonintact skin, and when touching items or
surfaces soiled with blood or other body fluids. Change gloves between
procedures. Wash hands immediately after removing gloves. (Note: Cover
open cuts or scrapes with a sterile bandage before donning gloves.)
11. Place glassware and plasticware contaminated by blood and other body
fluids in a disposable autoclave bag for decontamination by autoclaving,
or place them directly into a 10% bleach solution before reuse or
disposal. Place disposable materials such as gloves, mouthpieces, swabs,
and toothpicks that have come into contact with body fluids into a
disposable autoclave bag, and decontaminate before disposal.
12. To help prevent contamination by needlestick injuries, use only disposable
needles and lancets. Do not bend the needles and lancets. Needles and
lancets should be placed promptly in a labeled, puncture-resistant, leakproof
container and decontaminated, preferably by autoclaving.
13. Do not leave heat sources unattended.
14. Report all spills or accidents, no matter how minor, to the instructor.
15. Never work alone in the laboratory.
16. Remove protective clothing before leaving the laboratory.
,*Adapted from:
Biosafety in Microbiological and Biomedical Laboratories (BMBL), Fifth Edition. 2007. U.S. Government
Printing Office. Washington,
D.C. www.cdc.gov/od/OHS/biosfty/bmbl5/bmbl5toc.htm
Centers for Disease Control. 1996. “Universal Precautions for Prevention of Transmission of HIV and
Other Bloodborne Infec- tions.” Washington, D.C.
www.cdc.gov/ncidod/dhqp/bp_universal_precautions.html
Johnson, Ted, and Christine Case. 2010. Laboratory Experiments in Microbiology, Ninth Edition. San
Francisco: Pearson Benja- min Cummings.
School Chemistry Laboratory Safety Guide. 2006. U.S. Consumer Product Safety Commission.
Bethesda, MD. www.cpsc.gov/ CPSCPUB/PUBS/NIOSH2007107.pdf
AL Gr a wa n y
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The Axial
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2. The bone allows the passage of the Materials
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