QUESTIONS AND ANSWERS LATEST VERSION 2025 | VERIFIED
ANSWERS
Question 1
Which of the following is the first step in the decontamination process for
reusable surgical instruments?
A) Cleaning with enzymatic detergent.
B) Disinfection.
C) Manual scrubbing.
D) Gross soil removal at the point of use.
E) Ultrasonic cleaning.
Correct Answer: D) Gross soil removal at the point of use.
Rationale: Gross soil (e.g., blood, tissue) should be removed from
instruments at the point of use immediately after a procedure to
prevent it from drying and becoming more difficult to remove later,
which can hinder proper cleaning and sterilization.
Question 2
What is the primary purpose of a Material Safety Data Sheet (MSDS) or
Safety Data Sheet (SDS)?
A) To list all sterile processing department employees.
B) To provide instructions for instrument assembly.
C) To provide information about hazardous chemicals.
D) To track inventory of surgical supplies.
E) To record temperatures of sterilizers.
Correct Answer: C) To provide information about hazardous chemicals.
Rationale: MSDS/SDS documents are crucial for safety, providing
comprehensive information about hazardous substances, including
their properties, potential hazards, safe handling procedures, and
emergency measures.
Question 3
Which of the following describes the correct traffic flow in a sterile processing
department (SPD)?
A) Clean to dirty.
B) Sterile to dirty.
C) Dirty to clean.
D) Assembly to decontamination.
E) Storage to sterilization.
Correct Answer: C) Dirty to clean.
Rationale: The traffic flow in SPD should always be from dirty
,(decontamination) to clean (assembly) to sterile
(storage/sterilization) to prevent cross-contamination and maintain
sterile integrity.
Question 4
What is the purpose of ultrasonic cleaning in the decontamination area?
A) To dry instruments completely.
B) To sterilize heat-sensitive instruments.
C) To remove stubborn soil from instrument crevices.
D) To lubricate instruments before sterilization.
E) To manually scrub instruments.
Correct Answer: C) To remove stubborn soil from instrument crevices.
Rationale: Ultrasonic cleaners use high-frequency sound waves to
create cavitation, which dislodges microscopic debris from
instrument surfaces and crevices that may be difficult to reach with
manual cleaning.
Question 5
Which of the following types of water is used for the final rinse of
instruments in the decontamination area?
A) Tap water.
B) Hard water.
C) Deionized (DI) or reverse osmosis (RO) water.
D) Chlorinated water.
E) Saline solution.
Correct Answer: C) Deionized (DI) or reverse osmosis (RO) water.
Rationale: Deionized (DI) or reverse osmosis (RO) water is used for
the final rinse to remove mineral ions and other impurities that can
cause spotting, staining, or corrosion on instruments during
sterilization.
Question 6
What is the acceptable range for the relative humidity in the sterile storage
area?
A) Less than 20%.
B) 20% to 30%.
C) 30% to 60%.
D) 60% to 80%.
E) Greater than 80%.
Correct Answer: C) 30% to 60%.
Rationale: According to AAMI and other standards, the recommended
,relative humidity in sterile storage areas is typically 30% to 60% to
prevent moisture accumulation and bacterial growth on sterile
packages.
Question 7
Biological indicators (BIs) are used to:
A) Indicate if a package has been exposed to sterilant.
B) Provide an immediate pass/fail result for sterilization.
C) Verify the effectiveness of the sterilization process.
D) Track the expiration date of sterile items.
E) Measure the temperature inside the sterilizer chamber.
Correct Answer: C) Verify the effectiveness of the sterilization process.
Rationale: Biological indicators contain bacterial spores that are
highly resistant to sterilization. If the spores are killed, it provides
the highest level of assurance that the sterilization process was
effective in killing all microorganisms.
Question 8
Which of the following is the recommended minimum frequency for testing
the efficacy of an ultrasonic cleaner?
A) Daily.
B) Weekly.
C) Monthly.
D) Quarterly.
E) Annually.
Correct Answer: A) Daily.
Rationale: The efficacy of an ultrasonic cleaner should be tested daily,
typically using a foil test or a commercially prepared test strip, to
ensure it is functioning correctly and providing effective cavitation.
Question 9
The process of physically removing all visible soil from an item is called:
A) Disinfection.
B) Sterilization.
C) Cleaning.
D) Sanitization.
E) Antisepsis.
Correct Answer: C) Cleaning.
Rationale: Cleaning is the first and most crucial step in reprocessing,
defined as the removal of all visible and non-visible soil from an
item, usually with water and detergents or enzymatic products.
, Question 10
Which sterilization method is most commonly used for heat- and moisture-
stable medical devices?
A) Ethylene Oxide (EO) sterilization.
B) Hydrogen Peroxide Gas Plasma sterilization.
C) Dry Heat sterilization.
D) Steam sterilization.
E) Ozone sterilization.
Correct Answer: D) Steam sterilization.
Rationale: Steam sterilization (autoclaving) is the most common,
safest, and cost-effective method for items that can withstand high
temperatures and moisture. It effectively kills microorganisms by
denaturing proteins.
Question 11
When packaging instruments for sterilization, textiles (wraps) should be:
A) Damp to ensure proper moisture penetration.
B) Folded loosely to allow for maximum air removal.
C) Wrapped in multiple layers to protect sterility.
D) Placed in plastic bags before wrapping.
E) Secured tightly with tape on all sides.
Correct Answer: C) Wrapped in multiple layers to protect sterility.
Rationale: Textile wraps are typically used in two layers to create a
tortuous path for microorganisms, protecting the sterile contents
from environmental contamination after sterilization.
Question 12
Which of the following is a critical parameter for steam sterilization?
A) Sterilant concentration.
B) Aeration time.
C) Pressure.
D) Lumen size.
E) Relative humidity.
Correct Answer: C) Pressure.
Rationale: For steam sterilization, critical parameters include time,
temperature, and pressure. Pressure is essential for achieving the
necessary temperature within the chamber.
Question 13
What is the purpose of cleaning instruments in the "decontamination" area?
A) To render them sterile.