Lab 7 Microbial Genetics & Genetic BIO250
L
Engineering
Student Name: Monilola Anigala
Access Code (located on the underside of the lid of your lab kit): AC-3BMK7G
Pre-Lab Questions
1. Which DNA nitrogenous bases pair with each other? Which bases are purines, and which
are pyrimidines?
Adenine (A) always forms a base pair with thymine (T), while guanine
(G) always forms a base pair with cytosine (C). This specific pairing
pattern is crucial for the accurate replication and transmission of
genetic information.
2. How is DNA information used to make proteins? What are the steps of this process?
DNA's genetic instructions are converted into functional proteins through a
two-step process called gene expression: Transcription: DNA is copied into
RNA. Translation: RNA is translated into a sequence of amino acids, which
then assemble into a protein. This process allows cells to synthesize the
proteins they need for their specific functions.
3. Give an example of a scenario in which you would perform PCR vs a scenario in which you
would use recombinant DNA technology.
PCR is a technique that can be used to detect the presence of a virus
in a patient sample. By amplifying specific DNA sequences, PCR can
identify even very small amounts of viral DNA. This information can be
used to diagnose a viral infection and monitor the patient's response
, Lab 7 Microbial Genetics & Genetic BIO250
to treatment. L
Engineering
L
Engineering
Student Name: Monilola Anigala
Access Code (located on the underside of the lid of your lab kit): AC-3BMK7G
Pre-Lab Questions
1. Which DNA nitrogenous bases pair with each other? Which bases are purines, and which
are pyrimidines?
Adenine (A) always forms a base pair with thymine (T), while guanine
(G) always forms a base pair with cytosine (C). This specific pairing
pattern is crucial for the accurate replication and transmission of
genetic information.
2. How is DNA information used to make proteins? What are the steps of this process?
DNA's genetic instructions are converted into functional proteins through a
two-step process called gene expression: Transcription: DNA is copied into
RNA. Translation: RNA is translated into a sequence of amino acids, which
then assemble into a protein. This process allows cells to synthesize the
proteins they need for their specific functions.
3. Give an example of a scenario in which you would perform PCR vs a scenario in which you
would use recombinant DNA technology.
PCR is a technique that can be used to detect the presence of a virus
in a patient sample. By amplifying specific DNA sequences, PCR can
identify even very small amounts of viral DNA. This information can be
used to diagnose a viral infection and monitor the patient's response
, Lab 7 Microbial Genetics & Genetic BIO250
to treatment. L
Engineering
