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James Watson and Francis Crick - (answer)discovered configuration of the DNA molecule
Rosalind Franklin - (answer)did an X-ray crystallography of DNA. Died of cancer before getting honored
for the work.
Ray White - (answer)1980 describes first polymorphic RFLP marker
Alec Jeffreys - (answer)isolates DNA markers and calls them DNA fingerprints
Kary Mullis - (answer)developed PCR
Colin Pitchfork - (answer)Arrested and his DNA profile matched with the semen from both murders.
John M. Butler - (answer)THE leading expert on DNA typing
Tommy Lee Andrews - (answer)1st US conviction based on DNA fingerprinting
DNA - (answer)A complex molecule containing the genetic information that makes up the chromosomes.
30-40 thousand - (answer)Genes located throughout chromosomes
Extraction - (answer)-Process of releasing the DNA from the cells
-Separating DNA from proteins, the swab, dirt, etc.
Four main extraction techniques - (answer)1) organic
2) chelex
3) Silica based
,FRSC 2600 FINAL EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS
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4) differential extraction
Types of samples obtained - (answer)1) Blood
2) Semen
3) Bone
4) Hairs
5) Saliva
Why observe samples: - (answer)1) identify potential subjects
2) exonerate individuals
3) identify crime and casualty victims
4) establish paternity
5) match organ donors
RFLP - (answer)Restriction Fragment Length Polymorphism
1) isolation
2) cutting
3) sorting
4) Analyzing
Radioactive Phosphorus - (answer)used to tag DNA in Hershey and Chase's experiment
-32 probes are added to the membrane which bond to the precise DNA fragments making them
radioactive.
PCR - (answer)-polymerase chain reaction
- technique for making many copies of a defined segment of DNA.
, FRSC 2600 FINAL EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS
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Electrophoresis - (answer)method of separating serum proteins by electrical charge
Three possible outcomes of electrophoresis - (answer)1) Match
2) Exclusion
Arrestee Bill - (answer)1) all people arrested for felony charges will be required to give a DNA sample.
2) Can get a hit in those 90 days and use that information even if they aren't charged.
Jesse James - (answer)1) Historians say he died by being shot in the back by a member of his own outlaw
gang while straightening a picture on the wall of his home.
2) He was apparently buried on the family homestead near Kearney, MO. The remains were moved in
1902 and reburied next to his wife.
3) A team of crime-lab specialists conducted a study and concluded that the remains in the Missouri
grave marked "Jess W. James" do belong to the outlaw
The Romanovs - (answer)1) In order to prevent those remaining loyal to the Tsar from finding his
remains, the bodies were buried in a secret location.
2) Anna Anderson claimed to be Anastasia until her death in 1984.
3) In 1991, the remains of the family were exhumed in Siberia.
4) Two skeletons were missing Anastasia and the youngest son Alexei
5) In 1994, scientists were able to use modern DNA to analyze the evidence and determine whether
Anna Anderson was Anastasia Romanov.
Serology - (answer)Deals withe the examination and analysis of body fluids.
Properties of Blood - (answer)Blood Volume→ On average accounts for 8% of total body weight
5-6 L of blood for males
4-5 L of blood for females