Mastering Molecular Biology: Expert-Verified MB
ASCP Questions & Answers 2024–2025
Which of the following is not a component of a nucleotide?
Phosphate group
Anti-codon
Ribose sugar
Nitrogen base - correct answers ⬛
✓ A n t i - codon
According to Chargaff's rule of base pairing, adenine pairs with: - correct answers ⬛
✓Thymine
What genes would be screened in a breast cancer panel? - correct answers ⬛
✓ H E R 2 , ERBB2,
BRCA1
Next Generation Sequencing uses: - correct answers ⬛
⬛Short sequence reads
✓
,Purines and pyrimidines differ from each other in that: - correct answers ✓
⬛
⬛Purines have two
rings; pyrimidines have one ring
The purines are:
Cytosine and uracil
Adenine and thymine
Thymine and cytosine
Adenine and guanine - correct answers ⬛
✓ A d e n i n e and guanine
What is the rate of mutation per round of DNA replication?
1 in 1,000 base pairs
1 in 10,000 base pairs
1 in 1,000,000 base pairs
1 in 1,000,000,000 base pairs - correct answers ⬛
✓1 in 1,000,000,000 base pairs
The rate of DNA migration through an agarose gel during electrophoresis does not depend on
which of the following factors?
Net charge of the molecule
,Size of the molecule
Shape of the molecule
Nucleotide sequence of the molecule - correct answers ⬛
✓ N u c leo tid e sequence of the molecule
What are the phases in a qPCR Amplification Plot?
Initiation, exponential, plateau
Baseline, exponential, plateau
Baseline, threshold, exponential, plateau
Baseline, initiation, threshold, exponential, plateau - correct answers ⬛
✓ I nitiation, exponential,
plateau
Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'?
5'-GAC
3'-GAC
3'-CTG
5'-GTC - correct answers ⬛
✓3'-GAC
, A patient with impaired judgment, personality changes, signs of abnormal body movements and
depression comes to the physician's office for a follow-up visit. The physician suspects a single-
gene disorder may be the cause of those clinical manifestations. A blood specimen was then sent
to your clinical laboratory for mutation screening in the Huntington gene. Testing with standard
PCR indicates that patient has Huntington Disease, HD. Which of the following would be
consistent with this diagnosis?
25 CAG repeats in the Huntington gene
85 CAG repeats in the Huntington gene
25 CGA repeats in the Huntington gene
85 CGA repeats in the Huntington gene - correct answers ⬛
✓85 CAG repeats in the Huntington
gene
Which two HPV types are responsible for most cases of cervical cancer?
16 and 18
31 and 59
16 and 58
44 and 59 - correct answers ⬛
✓16 and 18
Replication forks, known as origins of DNA replication, are created by this enzyme:
ASCP Questions & Answers 2024–2025
Which of the following is not a component of a nucleotide?
Phosphate group
Anti-codon
Ribose sugar
Nitrogen base - correct answers ⬛
✓ A n t i - codon
According to Chargaff's rule of base pairing, adenine pairs with: - correct answers ⬛
✓Thymine
What genes would be screened in a breast cancer panel? - correct answers ⬛
✓ H E R 2 , ERBB2,
BRCA1
Next Generation Sequencing uses: - correct answers ⬛
⬛Short sequence reads
✓
,Purines and pyrimidines differ from each other in that: - correct answers ✓
⬛
⬛Purines have two
rings; pyrimidines have one ring
The purines are:
Cytosine and uracil
Adenine and thymine
Thymine and cytosine
Adenine and guanine - correct answers ⬛
✓ A d e n i n e and guanine
What is the rate of mutation per round of DNA replication?
1 in 1,000 base pairs
1 in 10,000 base pairs
1 in 1,000,000 base pairs
1 in 1,000,000,000 base pairs - correct answers ⬛
✓1 in 1,000,000,000 base pairs
The rate of DNA migration through an agarose gel during electrophoresis does not depend on
which of the following factors?
Net charge of the molecule
,Size of the molecule
Shape of the molecule
Nucleotide sequence of the molecule - correct answers ⬛
✓ N u c leo tid e sequence of the molecule
What are the phases in a qPCR Amplification Plot?
Initiation, exponential, plateau
Baseline, exponential, plateau
Baseline, threshold, exponential, plateau
Baseline, initiation, threshold, exponential, plateau - correct answers ⬛
✓ I nitiation, exponential,
plateau
Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'?
5'-GAC
3'-GAC
3'-CTG
5'-GTC - correct answers ⬛
✓3'-GAC
, A patient with impaired judgment, personality changes, signs of abnormal body movements and
depression comes to the physician's office for a follow-up visit. The physician suspects a single-
gene disorder may be the cause of those clinical manifestations. A blood specimen was then sent
to your clinical laboratory for mutation screening in the Huntington gene. Testing with standard
PCR indicates that patient has Huntington Disease, HD. Which of the following would be
consistent with this diagnosis?
25 CAG repeats in the Huntington gene
85 CAG repeats in the Huntington gene
25 CGA repeats in the Huntington gene
85 CGA repeats in the Huntington gene - correct answers ⬛
✓85 CAG repeats in the Huntington
gene
Which two HPV types are responsible for most cases of cervical cancer?
16 and 18
31 and 59
16 and 58
44 and 59 - correct answers ⬛
✓16 and 18
Replication forks, known as origins of DNA replication, are created by this enzyme:
