Chap 1 to 26 Included ✅
Concepts of Genetics, 13e (Klug)
Special Topics 1 CRISPR-Cas and Genome Editing Special Topics 1 to 6 ✅
1) CRISPR-Cas functions in bacteria to ________.
A) fight viruses
B) replicate viral DNA
C) edit genomes
D) code for restriction enzymes
Answer: A
Section: ST 1.1
Bloom's Taxonomy: Remembering/Understanding
2) Mechanisms of adaptive immunity include ________.
A) restriction enzymes
B) blocking phage adsorption
C) blocking phage DNA insertion
D) learning from past exposures
Answer: D
Section: ST 1.1
Bloom's Taxonomy: Remembering/Understanding
3) cas genes code for a variety of proteins, including ________.
A) RNases
B) polysaccharides
C) lysozymes
D) crRNAs
Answer: A
Section: ST 1.1
Bloom's Taxonomy: Remembering/Understanding
4) Which of the following is the correct order of steps for the CRISPR-Cas mechanism?
A) Cleavage, target interference, biogenesis
B) Spacer acquisition, biogenesis, target interference
C) Target interference, transcription, biogenesis
D) Transcription, cleavage, spacer acquisition
Answer: B
Section: ST 1.1
Bloom's Taxonomy: Remembering/Understanding
5) Type II CRISPR-Cas systems are the most widely used systems because ________.
A) they were discovered first
B) Streptococcus pyogenes is an important research organism
C) they use a nuclease complex
D) they only require a single protein, Cas9
Answer: D
Section: ST 1.1
Bloom's Taxonomy: Applying/Analyzing
1
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,6) Which of the following is required to efficiently target DNA in vitro using CRISPR-Cas9?
A) Cas9
B) tRNA
C) Target RNA
D) Native crRNA
Answer: A
Section: ST 1.2
Bloom's Taxonomy: Remembering/Understanding
7) Genome editing is based on ________.
A) acetylating specific DNA sequences with specific nucleases
B) methylating specific DNA sequences with specific proteins
C) targeting and manipulating specific DNA sequences with specific nucleases
D) transcribing specific DNA sequences with specific proteins
Answer: C
Section: ST 1.2
Bloom's Taxonomy: Remembering/Understanding
8) Which of the following is a pathway available to cells to repair double-stranded DNA breaks?
A) Homologous directed end-joining
B) Homologous end-joining
C) Nonhomologous directed repair
D) Nonhomologous end-joining
Answer: D
Section: ST 1.2
Bloom's Taxonomy: Remembering/Understanding
9) The primary benefit of CRISPR-Cas over TALENs and ZFNs is ________.
A) the ability of Cas9 to bind engineered sgRNA
B) the specificity of the nuclease
C) the speed of the process
D) the cost
Answer: A
Section: ST 1.2
Bloom's Taxonomy: Evaluating/Creating
10) A strategy to induce random insertions or deletions in a target sequence might use ________.
A) HDR to match damaged and undamaged chromosomes
B) NHEJ to ligate fragments
C) HDR and a donor template
D) NHEJ and Cas9
Answer: B
Section: ST 1.2
Bloom's Taxonomy: Evaluating/Creating
2
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,11) The use of CRISPR-Cas9 for in vivo genome editing in mammalian cells requires which of the
following?
A) Plasmid expression vector with a gene encoding Cas9
B) Plasmid expression vector with a gene encoding an sgRNA
C) A target sequence in the mammalian genome with an adjacent PAM sequence
D) Plasmid expression vectors with genes encoding Cas9, an sgRNA, and a target sequence in the
mammalian genome with an adjacent PAM sequence
Answer: D
Section: ST 1.2
Bloom's Taxonomy: Remembering/Understanding
12) When using CRISPR-Cas9 genome editing, if the goal is to create a nonfunctional allele, then an
artificial donor template ________ needed, and if the goal is to create a specific substitution, then an
artificial donor template ________ needed.
A) is; is not
B) is not; is not
C) is not; is
D) is; is
Answer: C
Section: ST 1.2
Bloom's Taxonomy: Remembering/Understanding
13) A scientist planned to edit mammalian gene X using CRISPR-Cas9 gene editing, so that the thirtieth
nucleotide in exon 1 of gene X was converted from a T to an A. However, when examining sequencing
results after conducting CRISPR-Cas9, they saw that gene X instead had a small deletion in exon 1. What
most likely led to this result?
A) Cas9 was not targeted to the appropriate gene.
B) An artificial donor template was not introduced into target cells.
C) sgRNA was not introduced into target cells.
D) Exon 1 was not present in the target cells.
Answer: B
Section: ST 1.2
Bloom's Taxonomy: Evaluating/Creating
14) Reverse genetics is a strategy with which researchers ________.
A) investigate the function of proteins
B) turn on a gene and monitor the effects
C) delete genes and monitor the effects
D) delete a genome and investigate the function of plasmids
Answer: C
Section: ST 1.3
Bloom's Taxonomy: Remembering/Understanding
3
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, 15) dCas9 is a useful tool in the CRISPR-Cas toolkit because it can ________.
A) deactivate the transcription of a gene
B) fluoresce and help researchers locate target sequences
C) bind DNA, but it cannot travel down the template strand
D) bind DNA, but it cannot cut DNA
Answer: D
Section: ST 1.3
Bloom's Taxonomy: Remembering/Understanding
16) All of the following are challenges in gene therapy EXCEPT ________.
A) unknown specific mutations
B) inaccessible tissues
C) unknown cell types
D) designing a strategy
Answer: D
Section: ST 1.3
Bloom's Taxonomy: Remembering/Understanding
17) Examples of modifications to Cas9 include all of the following EXCEPT ________.
A) epigenetic modifiers
B) fluorescent proteins
C) promotor domains
D) repression domains
Answer: C
Section: ST 1.3
Bloom's Taxonomy: Remembering/Understanding
18) The primary value of the CRISPR-Cas system in biotechnology is ________.
A) the removal of ineffective genes
B) simple modifications of genomes
C) rapid and cost-effective production of genetically modified organisms
D) addition of random indels
Answer: C
Section: ST 1.3
Bloom's Taxonomy: Remembering/Understanding
19) Applications of CRISPR-Cas in biotechnology include all of the following EXCEPT ________.
A) creating bioreactors to manufacture human proteins
B) modulating epigenetic regulation
C) increasing drought resistance of crops
D) engineering pigs that are resistance to PRRS
Answer: B
Section: ST 1.3
Bloom's Taxonomy: Applying/Analyzing
4
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Concepts of Genetics, 13e (Klug)
Special Topics 1 CRISPR-Cas and Genome Editing Special Topics 1 to 6 ✅
1) CRISPR-Cas functions in bacteria to ________.
A) fight viruses
B) replicate viral DNA
C) edit genomes
D) code for restriction enzymes
Answer: A
Section: ST 1.1
Bloom's Taxonomy: Remembering/Understanding
2) Mechanisms of adaptive immunity include ________.
A) restriction enzymes
B) blocking phage adsorption
C) blocking phage DNA insertion
D) learning from past exposures
Answer: D
Section: ST 1.1
Bloom's Taxonomy: Remembering/Understanding
3) cas genes code for a variety of proteins, including ________.
A) RNases
B) polysaccharides
C) lysozymes
D) crRNAs
Answer: A
Section: ST 1.1
Bloom's Taxonomy: Remembering/Understanding
4) Which of the following is the correct order of steps for the CRISPR-Cas mechanism?
A) Cleavage, target interference, biogenesis
B) Spacer acquisition, biogenesis, target interference
C) Target interference, transcription, biogenesis
D) Transcription, cleavage, spacer acquisition
Answer: B
Section: ST 1.1
Bloom's Taxonomy: Remembering/Understanding
5) Type II CRISPR-Cas systems are the most widely used systems because ________.
A) they were discovered first
B) Streptococcus pyogenes is an important research organism
C) they use a nuclease complex
D) they only require a single protein, Cas9
Answer: D
Section: ST 1.1
Bloom's Taxonomy: Applying/Analyzing
1
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,6) Which of the following is required to efficiently target DNA in vitro using CRISPR-Cas9?
A) Cas9
B) tRNA
C) Target RNA
D) Native crRNA
Answer: A
Section: ST 1.2
Bloom's Taxonomy: Remembering/Understanding
7) Genome editing is based on ________.
A) acetylating specific DNA sequences with specific nucleases
B) methylating specific DNA sequences with specific proteins
C) targeting and manipulating specific DNA sequences with specific nucleases
D) transcribing specific DNA sequences with specific proteins
Answer: C
Section: ST 1.2
Bloom's Taxonomy: Remembering/Understanding
8) Which of the following is a pathway available to cells to repair double-stranded DNA breaks?
A) Homologous directed end-joining
B) Homologous end-joining
C) Nonhomologous directed repair
D) Nonhomologous end-joining
Answer: D
Section: ST 1.2
Bloom's Taxonomy: Remembering/Understanding
9) The primary benefit of CRISPR-Cas over TALENs and ZFNs is ________.
A) the ability of Cas9 to bind engineered sgRNA
B) the specificity of the nuclease
C) the speed of the process
D) the cost
Answer: A
Section: ST 1.2
Bloom's Taxonomy: Evaluating/Creating
10) A strategy to induce random insertions or deletions in a target sequence might use ________.
A) HDR to match damaged and undamaged chromosomes
B) NHEJ to ligate fragments
C) HDR and a donor template
D) NHEJ and Cas9
Answer: B
Section: ST 1.2
Bloom's Taxonomy: Evaluating/Creating
2
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,11) The use of CRISPR-Cas9 for in vivo genome editing in mammalian cells requires which of the
following?
A) Plasmid expression vector with a gene encoding Cas9
B) Plasmid expression vector with a gene encoding an sgRNA
C) A target sequence in the mammalian genome with an adjacent PAM sequence
D) Plasmid expression vectors with genes encoding Cas9, an sgRNA, and a target sequence in the
mammalian genome with an adjacent PAM sequence
Answer: D
Section: ST 1.2
Bloom's Taxonomy: Remembering/Understanding
12) When using CRISPR-Cas9 genome editing, if the goal is to create a nonfunctional allele, then an
artificial donor template ________ needed, and if the goal is to create a specific substitution, then an
artificial donor template ________ needed.
A) is; is not
B) is not; is not
C) is not; is
D) is; is
Answer: C
Section: ST 1.2
Bloom's Taxonomy: Remembering/Understanding
13) A scientist planned to edit mammalian gene X using CRISPR-Cas9 gene editing, so that the thirtieth
nucleotide in exon 1 of gene X was converted from a T to an A. However, when examining sequencing
results after conducting CRISPR-Cas9, they saw that gene X instead had a small deletion in exon 1. What
most likely led to this result?
A) Cas9 was not targeted to the appropriate gene.
B) An artificial donor template was not introduced into target cells.
C) sgRNA was not introduced into target cells.
D) Exon 1 was not present in the target cells.
Answer: B
Section: ST 1.2
Bloom's Taxonomy: Evaluating/Creating
14) Reverse genetics is a strategy with which researchers ________.
A) investigate the function of proteins
B) turn on a gene and monitor the effects
C) delete genes and monitor the effects
D) delete a genome and investigate the function of plasmids
Answer: C
Section: ST 1.3
Bloom's Taxonomy: Remembering/Understanding
3
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, 15) dCas9 is a useful tool in the CRISPR-Cas toolkit because it can ________.
A) deactivate the transcription of a gene
B) fluoresce and help researchers locate target sequences
C) bind DNA, but it cannot travel down the template strand
D) bind DNA, but it cannot cut DNA
Answer: D
Section: ST 1.3
Bloom's Taxonomy: Remembering/Understanding
16) All of the following are challenges in gene therapy EXCEPT ________.
A) unknown specific mutations
B) inaccessible tissues
C) unknown cell types
D) designing a strategy
Answer: D
Section: ST 1.3
Bloom's Taxonomy: Remembering/Understanding
17) Examples of modifications to Cas9 include all of the following EXCEPT ________.
A) epigenetic modifiers
B) fluorescent proteins
C) promotor domains
D) repression domains
Answer: C
Section: ST 1.3
Bloom's Taxonomy: Remembering/Understanding
18) The primary value of the CRISPR-Cas system in biotechnology is ________.
A) the removal of ineffective genes
B) simple modifications of genomes
C) rapid and cost-effective production of genetically modified organisms
D) addition of random indels
Answer: C
Section: ST 1.3
Bloom's Taxonomy: Remembering/Understanding
19) Applications of CRISPR-Cas in biotechnology include all of the following EXCEPT ________.
A) creating bioreactors to manufacture human proteins
B) modulating epigenetic regulation
C) increasing drought resistance of crops
D) engineering pigs that are resistance to PRRS
Answer: B
Section: ST 1.3
Bloom's Taxonomy: Applying/Analyzing
4
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