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Classification of bacteria does what?
Ans: groups species according to evolutionary relationships
Quiz Identification of bacteria does what?
Ans: relies on tests that may have little to do with evolutionary relationships
Quiz Specificity
Ans: the ability of a test to identify a single pathogen, high specificity reduces the number of
false positive results
,Quiz Sensitivity
Ans: the smallest quantity of a pathogen (or product of a pathogen) that a test can identify,
high sensitivity reduces number of false negative results
Quiz Morphology
Ans: shape, size, presence of fijmbraie, flagella, endospores etc., more useful in identifying
eukaryotic organisms
Quiz Differential Staining
Ans: Allows for the identification of cells using more then one chemical or stain to identify the
structural differences of the cells in the sample
Quiz Examples of differential staining include what?
Ans: gram staining, acid-fast staining, Schaeffer-Fulton endospore stain, flagella staining
Quiz What colour do gram-positive cells appear? Negative?
Ans: purple, pink
Quiz In addition to using gram staining what other test is used to identify N.gonorrhoeae and
why?
, Ans: oxidase test, in order to differentiate between the gonorrhoea and other bacteria
Quiz Biochemical results
Ans: Most rely on detecting the presence or absence of a particular enzyme or biochemical
pathway such as catalase
Quiz The Enterotube 2 is an example of what test? What does it do?
Ans: rapid biochemical identification system, each compartment is scored and the pre assigned
code that is produced identifies the most likely bacteria present
Quiz Serology
Ans: Study of antigen-antibody reactions in vitro
Quiz Serological tests usually have high _______________, because epitopes are very often
specific to ________ ______ of bacteria
Ans: specificity, one type
Quiz ELISA (enzyme-linked immunosorbent assay)
Ans: - Known antibodies bound to microplate well
- Unknown sample of bacteria added