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ASCP-CHEMISTRY SECTION 2025
BRAND NEW ACTUAL EXAM WITH
ANSWERS.
In the Jendrassik-Grof reaction for total bilirubin, bilirubin reacts
wth diazotized sulfanilic acid to form:
A. diazo bilirubin
B. biliverdin
C. azobilirubin
D. bilirubin glucurnide - correct answer -A. diazo bilirubin
In the assay of lactate dehydrogenase, which of the following
products if actually measured?
A. NADH
B. ATP
C. lactic acid
D. pyruvic acid - correct answer -C. lactic acid
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In the assay of lactate dehydrogenase (LD), the reaction is
dependent upon which of the following coenzyme systems?
A. NAD/NADH
B. ATP/ADP
C. FE++/Fe+++
D. Cu/Cu++ - correct answer -A. NAD/NADH
Refer to illustration:
This represents the change in absorbance at 340 nm over a
period of 8 minutes in an assay for lactate dehydrogenase. True
statments abut this figure include:
A. the reaction follows zero order kinetics between 5 and 8
minutes
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B. the reaction is proceeding from lactate pyruvase
C. nonlinearity after 6 minutes is due to substrate exhaustion
D. the change in absrbance is due to reduction of NAD and NADH
- correct answer -A. the reaction follows zero order kinetics
between 5 and 8 minutes
The International Federation for Clinical Chemistry (IFCC)
recommends the use of methods such as the Bessery-Lowry-
Brock method for determining alkaline phosphatase activity. The
substrate used in this type of method is:
A. monophosphate
B. phenylphosphate
C. disodium phenylphosphate
D. para-nitrophenylphosphate - correct answer -C. disodium
phenylphosphate
Which of the following is a glycolytic enzyme that catalyzes the
cleavage of fructose-1, 6-diphosphate to glyceraldehyde-3-
phosphate and dihydroxyacetone phosphate?
A. aldolase
B. phosphofructokinase
ASCP-CHEMISTRY SECTION 2025
BRAND NEW ACTUAL EXAM WITH
ANSWERS.
In the Jendrassik-Grof reaction for total bilirubin, bilirubin reacts
wth diazotized sulfanilic acid to form:
A. diazo bilirubin
B. biliverdin
C. azobilirubin
D. bilirubin glucurnide - correct answer -A. diazo bilirubin
In the assay of lactate dehydrogenase, which of the following
products if actually measured?
A. NADH
B. ATP
C. lactic acid
D. pyruvic acid - correct answer -C. lactic acid
, Page | 2
In the assay of lactate dehydrogenase (LD), the reaction is
dependent upon which of the following coenzyme systems?
A. NAD/NADH
B. ATP/ADP
C. FE++/Fe+++
D. Cu/Cu++ - correct answer -A. NAD/NADH
Refer to illustration:
This represents the change in absorbance at 340 nm over a
period of 8 minutes in an assay for lactate dehydrogenase. True
statments abut this figure include:
A. the reaction follows zero order kinetics between 5 and 8
minutes
, Page | 3
B. the reaction is proceeding from lactate pyruvase
C. nonlinearity after 6 minutes is due to substrate exhaustion
D. the change in absrbance is due to reduction of NAD and NADH
- correct answer -A. the reaction follows zero order kinetics
between 5 and 8 minutes
The International Federation for Clinical Chemistry (IFCC)
recommends the use of methods such as the Bessery-Lowry-
Brock method for determining alkaline phosphatase activity. The
substrate used in this type of method is:
A. monophosphate
B. phenylphosphate
C. disodium phenylphosphate
D. para-nitrophenylphosphate - correct answer -C. disodium
phenylphosphate
Which of the following is a glycolytic enzyme that catalyzes the
cleavage of fructose-1, 6-diphosphate to glyceraldehyde-3-
phosphate and dihydroxyacetone phosphate?
A. aldolase
B. phosphofructokinase
