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Straighterline Micro 250 Quiz 6 prep
Questions with Detailed Verified Answers
A microbiologist would like to use a noncompetent genus of streptococcal bacteria,
Enterococcus faecalis, as a cloning host to express genes from Streptococcus
pneumoniae, which is naturally competent. Is this possible?
Select one:
a. Yes; electroporation or chemical transformation can be used to make Enterococcus
competent, and then genes from Streptococcus can be introduced via a cloning
vector.
b. Yes; electroporation or chemical transformation can be used to make non-
competent Streptococcus mutants, from which genes can be inserted into a cloning
vector and introduced into Enterococcus.
c. No; competence factor is an essential protein that enables the uptake of foreign
DNA, therefore a cloning host such as Enterococcus that lacks competence factor
protein is unable to be transformed by electroporation or chemical transformation.
d. Yes; since the cloning host and the DNA to be introduced are both from bacteria th
Ans: Yes; electroporation or chemical transformation can be used to
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make Enterococcus competent, and then genes from Streptococcus can be introduced
via a cloning vector.
The three steps that take place in each cycle during PCR occur in which order?
Select one:
a. DNA annealing, denaturation, and synthesis
b. DNA denaturation, annealing, and synthesis
c. DNA synthesis, denaturation, and annealing
d. None of the choices are correct. Ans: DNA denaturation, annealing, and synthesis
In order to express eukaryotic genes in a bacterium, the __________ must first be
removed.
Select one:
a. introns
b. exons
c. enhancers
d. 3' poly A sequence Ans: introns
ORF refers to
Select one:
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a. the location of the start site of transcription.
b. the region of a gene that codes for a protein or a functional RNA
product.
c. the region of a gene that serves as the promoter.
d. the region between the ribosome binding site and translation initiation Ans: the
region of a gene that codes for a protein or a functional
Which of the following is a technology that can be used to identify the DNA to which a
protein binds?
Select one:
a. ChIP-Chip and ChIP-Seq analysis
b. RNA-seq and microarray analysis
c. PCR and RT-PCR analysis
d. Isoelectric focusing and 2D gel electrophoresis analysis Ans: ChIP-Chip and ChIP-
Seq analysis
The oxidized form of inorganic iron that results from microbial oxidative processes is
______.
Select one:
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a. ferrous ion
b. ferric ion
c. sulfate ion
d. nitrate ion Ans: ferric ion
Less than ______% of all microbial species have been cultured, identified, and
studied.
Select one:
a. 1
b. 2
c. 5
d. 10 Ans: 1
Which of the following microbes can oxidize ferrous iron while using chlorate or
perchlorate as the electron acceptor?
Select one:
a. Pseudomonas denitrificans
b. Thiobacillus ferrooxidans
Straighterline Micro 250 Quiz 6 prep
Questions with Detailed Verified Answers
A microbiologist would like to use a noncompetent genus of streptococcal bacteria,
Enterococcus faecalis, as a cloning host to express genes from Streptococcus
pneumoniae, which is naturally competent. Is this possible?
Select one:
a. Yes; electroporation or chemical transformation can be used to make Enterococcus
competent, and then genes from Streptococcus can be introduced via a cloning
vector.
b. Yes; electroporation or chemical transformation can be used to make non-
competent Streptococcus mutants, from which genes can be inserted into a cloning
vector and introduced into Enterococcus.
c. No; competence factor is an essential protein that enables the uptake of foreign
DNA, therefore a cloning host such as Enterococcus that lacks competence factor
protein is unable to be transformed by electroporation or chemical transformation.
d. Yes; since the cloning host and the DNA to be introduced are both from bacteria th
Ans: Yes; electroporation or chemical transformation can be used to
, Page | 2
make Enterococcus competent, and then genes from Streptococcus can be introduced
via a cloning vector.
The three steps that take place in each cycle during PCR occur in which order?
Select one:
a. DNA annealing, denaturation, and synthesis
b. DNA denaturation, annealing, and synthesis
c. DNA synthesis, denaturation, and annealing
d. None of the choices are correct. Ans: DNA denaturation, annealing, and synthesis
In order to express eukaryotic genes in a bacterium, the __________ must first be
removed.
Select one:
a. introns
b. exons
c. enhancers
d. 3' poly A sequence Ans: introns
ORF refers to
Select one:
, Page | 3
a. the location of the start site of transcription.
b. the region of a gene that codes for a protein or a functional RNA
product.
c. the region of a gene that serves as the promoter.
d. the region between the ribosome binding site and translation initiation Ans: the
region of a gene that codes for a protein or a functional
Which of the following is a technology that can be used to identify the DNA to which a
protein binds?
Select one:
a. ChIP-Chip and ChIP-Seq analysis
b. RNA-seq and microarray analysis
c. PCR and RT-PCR analysis
d. Isoelectric focusing and 2D gel electrophoresis analysis Ans: ChIP-Chip and ChIP-
Seq analysis
The oxidized form of inorganic iron that results from microbial oxidative processes is
______.
Select one:
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a. ferrous ion
b. ferric ion
c. sulfate ion
d. nitrate ion Ans: ferric ion
Less than ______% of all microbial species have been cultured, identified, and
studied.
Select one:
a. 1
b. 2
c. 5
d. 10 Ans: 1
Which of the following microbes can oxidize ferrous iron while using chlorate or
perchlorate as the electron acceptor?
Select one:
a. Pseudomonas denitrificans
b. Thiobacillus ferrooxidans
