MCB 151 EXAM 1 QUESTIONS AND ANSWERS 100% PASS
Lab Skills: convert .2 ml to microliters - ANSWER 200 ul
Lab Skills: This type of molecule cannot form hydrogen bonds with water molecules and
it repels water molecules. - ANSWER characteristics of hydrophobic molecules
Lab Skills: Your total magnification on a light microscope if you are using the 100X
objective - ANSWER 1000X
Lab Skills: 0.0001 expressed in scientific notation - ANSWER 1.0 * 10^-4
Lab Skills: Formula used to make single step dilutions - ANSWER C1V1 = C2V2
Bacteria: cocci, bacilli, spirochete - ANSWER bacterial cell shapes
Bacteria: A visible cluster of cells on the surface of solid medium - ANSWER a colony
Bacteria: Culture containing only one type of microorganism - ANSWER pure culture
Bacteria: The type of plate used to generate countable colonies from a diluted culture -
ANSWER spread plate
Bacteria: Probability that a single cell will form a colony - ANSWER EOP (Efficiency of
plating)
Enzymes: Lowers the activation energy of chemical reactions - ANSWER A catalyst
,Enzymes: An activated intermediate that must be formed for a chemical reaction to
occur. - ANSWER the transition state
Enzymes: The enzyme that catalyzes the hydrolysis of lactose to glucose and galactose -
ANSWER B-galactosidase
Enzymes: A structural analog of lactose - ANSWER ONPG
Enzymes: raises the pH of the reaction to 11 and inactivates the enzyme
(B-galactosidase) - ANSWER Na2CO3
DNA: The restriction enzymes used in this exercise - ANSWER EcoRV and PstI
DNA: The types of cuts produced by restriction enzymes. - ANSWER staggered and
blunt
DNA: Covalent joining of two DNA molecules. - ANSWER ligation
DNA: An enzyme that cuts within a nucleic acid sequence. - ANSWER endonuclease
DNA: The purpose of the following reagents in chromosomal DNA prep: Sodium dodecyl
sulfate Proteinase K 95% Ethanol EDTA - ANSWER lyses of the cell membrane
degradation of proteins precipitation of the DNA protects from contaminating nucleases
Electrophoresis: The charge of DNA - ANSWER negative
Electrophoresis: A planar molecule that inserts itself between the stacked bases in the
DNA double helix. - ANSWER ethidium bromide
Electrophoresis: The plasmid used in this exercise - ANSWER pBR322
, Electrophoresis: Standard used on your gel - ANSWER lambda DNA
Electrophoresis: A physical map of DNA showing the relative positions of restriction
sites and the distance in kb between the restriction sites. - ANSWER a restriction map
True or false?
When observing bacteria with brightfield microscopy, dyes are usually needed because
bacteria cells have a high water content and lack pigments. - ANSWER TRUE
The correct sequence of steps for observing a bacterial cell slide under the microscope
is:
1.stain bacterial cells
2.place slide on stage
3.watch the objective from the side
4.raise the stage using coarse focus knob
5.focus using coarse focus knob
6.rotate nosepiece so that 10X objective is over the slide
7.focus using fine focus knob
A) 2,6,1,3,4,7,5
B) 1,2,6,3,4,5,7
C) 2,6,1,3,4,5,7
D) 1,2,6,3,4,7,5
E) 1,2,3,4,5,6,7 - ANSWER B) 1,2,6,3,4,5,7
What are the three basic shapes of most bacteria?
Lab Skills: convert .2 ml to microliters - ANSWER 200 ul
Lab Skills: This type of molecule cannot form hydrogen bonds with water molecules and
it repels water molecules. - ANSWER characteristics of hydrophobic molecules
Lab Skills: Your total magnification on a light microscope if you are using the 100X
objective - ANSWER 1000X
Lab Skills: 0.0001 expressed in scientific notation - ANSWER 1.0 * 10^-4
Lab Skills: Formula used to make single step dilutions - ANSWER C1V1 = C2V2
Bacteria: cocci, bacilli, spirochete - ANSWER bacterial cell shapes
Bacteria: A visible cluster of cells on the surface of solid medium - ANSWER a colony
Bacteria: Culture containing only one type of microorganism - ANSWER pure culture
Bacteria: The type of plate used to generate countable colonies from a diluted culture -
ANSWER spread plate
Bacteria: Probability that a single cell will form a colony - ANSWER EOP (Efficiency of
plating)
Enzymes: Lowers the activation energy of chemical reactions - ANSWER A catalyst
,Enzymes: An activated intermediate that must be formed for a chemical reaction to
occur. - ANSWER the transition state
Enzymes: The enzyme that catalyzes the hydrolysis of lactose to glucose and galactose -
ANSWER B-galactosidase
Enzymes: A structural analog of lactose - ANSWER ONPG
Enzymes: raises the pH of the reaction to 11 and inactivates the enzyme
(B-galactosidase) - ANSWER Na2CO3
DNA: The restriction enzymes used in this exercise - ANSWER EcoRV and PstI
DNA: The types of cuts produced by restriction enzymes. - ANSWER staggered and
blunt
DNA: Covalent joining of two DNA molecules. - ANSWER ligation
DNA: An enzyme that cuts within a nucleic acid sequence. - ANSWER endonuclease
DNA: The purpose of the following reagents in chromosomal DNA prep: Sodium dodecyl
sulfate Proteinase K 95% Ethanol EDTA - ANSWER lyses of the cell membrane
degradation of proteins precipitation of the DNA protects from contaminating nucleases
Electrophoresis: The charge of DNA - ANSWER negative
Electrophoresis: A planar molecule that inserts itself between the stacked bases in the
DNA double helix. - ANSWER ethidium bromide
Electrophoresis: The plasmid used in this exercise - ANSWER pBR322
, Electrophoresis: Standard used on your gel - ANSWER lambda DNA
Electrophoresis: A physical map of DNA showing the relative positions of restriction
sites and the distance in kb between the restriction sites. - ANSWER a restriction map
True or false?
When observing bacteria with brightfield microscopy, dyes are usually needed because
bacteria cells have a high water content and lack pigments. - ANSWER TRUE
The correct sequence of steps for observing a bacterial cell slide under the microscope
is:
1.stain bacterial cells
2.place slide on stage
3.watch the objective from the side
4.raise the stage using coarse focus knob
5.focus using coarse focus knob
6.rotate nosepiece so that 10X objective is over the slide
7.focus using fine focus knob
A) 2,6,1,3,4,7,5
B) 1,2,6,3,4,5,7
C) 2,6,1,3,4,5,7
D) 1,2,6,3,4,7,5
E) 1,2,3,4,5,6,7 - ANSWER B) 1,2,6,3,4,5,7
What are the three basic shapes of most bacteria?
