ASCP Recall EXAM QUESTIONS AND DETAILED ANSWERS
ASCP Recall EXAM QUESTIONS AND DETAILED ANSWERS
Procedure #1 detected 50/100 true positives & 100/100 true negatives. Procedure #2 detected
80/100 true positives and 70/100 true negatives. Answer✔✔ − Procedure 2 is more sensitive
(refers to % of true positives that are accurately identified as positives). Specificity refers to # of
true negatives identified as negative.
TP/TP+FN= Answer✔✔ − Sensitivity
TN/TN+FP= Answer✔✔ − Specificity
What might the following indicate in urine: RBCs, WBCs, nitrite, and bacteria? Answer✔✔ −
Pyelonephritis (UTI); WBCs and nitrites in urine are classical indication of bacterial infection
(UTI)
,ASCP Recall EXAM QUESTIONS AND DETAILED ANSWERS
Why is albumin the first protein to be detected in tests for renal failure? Answer✔✔ − It's
molecular size is the smallest
Cortisol excess will result in: Answer✔✔ − Elevated glucose in blood; cortisol counteracts
insulin
What is the reason for the following discrepency? Anti A: 3+
Anti B: 3+
A1: 3+
B cells: 0 Answer✔✔ − Patient may be A2B
This spiral form organism is seen in urine and cultured on fletcher's medium Answer✔✔ −
Leptospira
Organism that gives off a bleach−like odor in culture: Answer✔✔ − Eikenella (GN pits agar)
Presence of Rheumatoid factor in blood may result in false positives for what test? Answer✔✔
− VDRL; nonspecific screening test for syphillis
,ASCP Recall EXAM QUESTIONS AND DETAILED ANSWERS
Disease associated with the following: increased TSH increased T3 and increased FT4:
Answer✔✔ − Pituitary tumor
If excess PTH is released, what woulde you find elevated amounts of in serum? Answer✔✔ −
Calcium
Mucoid pink colonies on plate that produce gase, indole positive, yellow/yellow on TSI
Answer✔✔ − Klebsiella oxytoca (Klebsiella pneumo is indole negative)
PAD positive, indole positive, organism stains gram negative. What is it? Answer✔✔ − Proteus
vulgaris (p. mirabilis is indole negative)
Curved gram negative bacilli. Cultured form GI tract of persons with ulcers. What test would
you do to confirm its identity? Answer✔✔ − Test for Urease. Most likely H. pylori which is
curved, infects GI tract, and the urease activity can cause ulcers.
Enzyme controls run on a machine gives result around −3SD. Samples run on the same machine
give results <1 SD. What is the problem? Answer✔✔ − Controls were left @ room temperature
for several days.
, ASCP Recall EXAM QUESTIONS AND DETAILED ANSWERS
HIV−1 and HIV−2 combination ELISA test is positive in a patient with symptoms of immune
deficiency. Western blot was inconclusive for HIV−1. What do you do next? Answer✔✔ − DO
HIV−2 western blot.
What are steps of PCR? Answer✔✔ − Denaturation, annealing, and transcription.
RAST test detects what? Answer✔✔ − IgE to particular antigens (RIST is for total IgE)
After collecting a blood sample in an EDTA tube for CBC you find that the Hct is very high (68%).
What should you do next? Answer✔✔ − Report results. Always use EDTA for CBC specimens.
When you conduct a procedure using fluorescence it is important to protect yourself from:
Answer✔✔ − Exciting light; high energy light produced by the machine
Blood was collected Nov. 1st then frozen with glycol on Nov. 5th. What is the new expiration
date? Answer✔✔ − Nov. 1 10 years from now.
A person was successfully treated for syphillis 12 years ago. he just came in worried about being
reinfected. what would you look for in his blood? Answer✔✔ − VDRL; TPA is not correct
because it can remain active for the life of the patient.
ASCP Recall EXAM QUESTIONS AND DETAILED ANSWERS
Procedure #1 detected 50/100 true positives & 100/100 true negatives. Procedure #2 detected
80/100 true positives and 70/100 true negatives. Answer✔✔ − Procedure 2 is more sensitive
(refers to % of true positives that are accurately identified as positives). Specificity refers to # of
true negatives identified as negative.
TP/TP+FN= Answer✔✔ − Sensitivity
TN/TN+FP= Answer✔✔ − Specificity
What might the following indicate in urine: RBCs, WBCs, nitrite, and bacteria? Answer✔✔ −
Pyelonephritis (UTI); WBCs and nitrites in urine are classical indication of bacterial infection
(UTI)
,ASCP Recall EXAM QUESTIONS AND DETAILED ANSWERS
Why is albumin the first protein to be detected in tests for renal failure? Answer✔✔ − It's
molecular size is the smallest
Cortisol excess will result in: Answer✔✔ − Elevated glucose in blood; cortisol counteracts
insulin
What is the reason for the following discrepency? Anti A: 3+
Anti B: 3+
A1: 3+
B cells: 0 Answer✔✔ − Patient may be A2B
This spiral form organism is seen in urine and cultured on fletcher's medium Answer✔✔ −
Leptospira
Organism that gives off a bleach−like odor in culture: Answer✔✔ − Eikenella (GN pits agar)
Presence of Rheumatoid factor in blood may result in false positives for what test? Answer✔✔
− VDRL; nonspecific screening test for syphillis
,ASCP Recall EXAM QUESTIONS AND DETAILED ANSWERS
Disease associated with the following: increased TSH increased T3 and increased FT4:
Answer✔✔ − Pituitary tumor
If excess PTH is released, what woulde you find elevated amounts of in serum? Answer✔✔ −
Calcium
Mucoid pink colonies on plate that produce gase, indole positive, yellow/yellow on TSI
Answer✔✔ − Klebsiella oxytoca (Klebsiella pneumo is indole negative)
PAD positive, indole positive, organism stains gram negative. What is it? Answer✔✔ − Proteus
vulgaris (p. mirabilis is indole negative)
Curved gram negative bacilli. Cultured form GI tract of persons with ulcers. What test would
you do to confirm its identity? Answer✔✔ − Test for Urease. Most likely H. pylori which is
curved, infects GI tract, and the urease activity can cause ulcers.
Enzyme controls run on a machine gives result around −3SD. Samples run on the same machine
give results <1 SD. What is the problem? Answer✔✔ − Controls were left @ room temperature
for several days.
, ASCP Recall EXAM QUESTIONS AND DETAILED ANSWERS
HIV−1 and HIV−2 combination ELISA test is positive in a patient with symptoms of immune
deficiency. Western blot was inconclusive for HIV−1. What do you do next? Answer✔✔ − DO
HIV−2 western blot.
What are steps of PCR? Answer✔✔ − Denaturation, annealing, and transcription.
RAST test detects what? Answer✔✔ − IgE to particular antigens (RIST is for total IgE)
After collecting a blood sample in an EDTA tube for CBC you find that the Hct is very high (68%).
What should you do next? Answer✔✔ − Report results. Always use EDTA for CBC specimens.
When you conduct a procedure using fluorescence it is important to protect yourself from:
Answer✔✔ − Exciting light; high energy light produced by the machine
Blood was collected Nov. 1st then frozen with glycol on Nov. 5th. What is the new expiration
date? Answer✔✔ − Nov. 1 10 years from now.
A person was successfully treated for syphillis 12 years ago. he just came in worried about being
reinfected. what would you look for in his blood? Answer✔✔ − VDRL; TPA is not correct
because it can remain active for the life of the patient.
