Actual Frequently Tested Exam Questions With
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1. Pre-analytical - ANSWER anything that happens before patient sample is
tested
-patient preparation, communication with sample collectors
-sample collection
-sample transport to lab
2. Analytical - ANSWER performing testing on patient sample
-required/ requested testing is done
3. Post-analytical - ANSWER after testing has occurred on patient sample
-recording results
-supervisors review results
-report results on patient's record
4. Specimen container label - ANSWER -patient name
-patient ID number
-location (room #, floor, outpatient address)
,-ordering physician
-culture site (specimen source)
-date and time of collection
5. Specimen requisition form - ANSWER -information needs to match sample
container: patient name, ID #, location, physician name, culture site, date and
time of collection
-patient age and sex
-date and time of specimen arrival to lab
-ongoing clinical diagnoses of patient
-testing required
-name of person ordering test (know who to contact)
-any antimicrobial agents patient's receiving
6. Specimen collection protocols - ANSWER -collection site: site that is
affected by disease, contains pathogen and not normal biota
-avoid normal biota: cleanse collection site, use needle aspiration if necessary
(normal biota interferes with testing and makes pathogen difficult to isolate)
-timing: collect sample that contains bacteria based on phase of illness, acute
samples contain more bacteria than convalescent, first morning urine and sputum
are most concentrated
-labeling: all required information present and matching requisition form
-transport: package in container with transport media to maintain viability and
prevent overgrowth, transport promptly (2 hours max)
,7. Bacterial transport medias - ANSWER -Stuart and Aime are most common
-Cary blair used for stools
-anaerobic transport media
-direct bedside inoculation kits for fastidious bacteria
8. Viral transport medias - ANSWER -isotonic solution, protein and
antibacterial and anti-fungal ants
9. Parasitic transport medias - ANSWER series of 3 stools in clean, dry tightly
sealed waterproof container
-polyvinyl alcohol and 10% formalin preservative, deliver to lab ASAP
10. Specimen storage - ANSWER -bacteria: store in fridge or RT, use
preservatives for urine and stools, use anticoagulant for samples containing blood
-viral: process ASAP or store in fridge at 4C, if storing >4 days freeze at -70 to -80
11. Sample rejection criteria - ANSWER -sample container and requisition form
info don't match
-request form has insufficient info
-duplicate specimen
-specimen is unsuitable for testing or culture that was requested (e.g. wrong
sample was collected)
-insufficient quantity of sample
-leaking container introduce environmental bacteria
, -delay between collection and receipt of sample to lab
-inappropriate transport media
-transported at wrong temp
12. Sample rejection protocol - ANSWER -call unit that ordered the testing:
resolve issue or request new specimen, hold specimen
-do not discard until requesting physician has been consulted
-if physician insists that specimen is used (i.e. cannot be recollected), include
disclaimer in statement report that you are not confident of results for this reason
13. Specimen priority - ANSWER process all specimens ASAP (preferably within
1-2 hours of collection)
-priority 1 (critical/ invasive species): potentially threatening infection, samples
that require invasive collection, normally sterile body fluids (blood, CSF),
organisms with limited viability, amniotic fluid, blood, brain, CSF, heart and
pericardial fluids
-priority 2 (unpreserved specimens): process quickly before sample degradation
occurs, body fluids, bone, wound drainage, feces, sputum, tissue
-priority 3 (quantitation required): things that need to be counted before
organisms multiply, catheter tip, urine, tissues
-priority 4 (preserved samples): feces/ urine in preservative, swabs in holding
media
14. Reporting sample results - ANSWER -STAT/ urgent specimen report: call
physician as soon as you have any information about the sample to share
-only present facts, no interpretations