BIOL 3110 Final Exam Questions and Answers (Solved Papers)

BIOL 3110 Final Exam Questions and
Answers (Solved Papers)
Cellular reverse transcriptase describes the enzymatic
activity of which of the following enzymes?


a. DNA polymerase.
b. Exonuclease.
c. Telomerase.
d. Primase.
e. Topoisomerase. - Correct Answers ✅c. Telomerase.


Which of the following activities is NOT shared by E. coli DNA
polymerase I and DNA polymerase III?


a. A proofreading activity.
b. A 5' to 3' polymerase activity.
c. A processive and fast rate of DNA synthesis.
d. A 5' to 3' exonuclease activity.
e. A 3' to 5' exonuclease activity. - Correct Answers ✅d. A
5' to 3' exonuclease activity.


Which are the following statements BEST describes lagging
strand synthesis in DNA
replication?


a. Is coordinated with leading strand synthesis.

,BIOL 3110 Final Exam Questions and
Answers (Solved Papers)
b. Involves DNA polymerization in the 5' to 3' direction.
c. Uses an RNA primer.
d. Requires DNA ligase.
e. All of the above are characteristics of lagging strand
synthesis. - Correct Answers ✅e. All of the above are
characteristics of lagging strand synthesis.


In growing cells, unrepaired spontaneous deamination of
cytosine is associated with:


a. No change in DNA sequence because base pairing is
unaltered.
b. A change in DNA sequence from a CG base pair to a TA
base pair.
c. A change in DNA sequence from a CG base pair to an AT
base pair.
d. A change in DNA sequence from a CG base pair to a GC
base pair.
e. None of the above. - Correct Answers ✅b. A change in
DNA sequence from a CG base pair to a TA base pair.


Base excision repair involves:
a. Cleavage of both strands of the DNA.


b. Repair by DNA polymerase III.

,BIOL 3110 Final Exam Questions and
Answers (Solved Papers)
c. The action of the ABC excinuclease.
d. Formation of an apurinic or apyrimidic site.
e. All of the above. - Correct Answers ✅d. Formation of an
apurinic or apyrimidic site.


Which of the following statements about genetic mutations
and DNA repair is
TRUE?


a. Errors in DNA replication can cause mutations.
b. Pyrimidine dimers are repaired using the mismatch repair
pathway.
c. The Ames test identifies mutagens that cause double
strand DNA breaks.
d. All cellular repair pathways require a DNA polymerase.
e. Base excision repair can repair double strand DNA breaks. -
Correct Answers ✅a. Errors in DNA replication can cause
mutations.


Which of the following mutations in the cis-elements of the
lac operon will be associated with the HIGHEST level of
transcription, when E. coli is grown in the presence of lactose
but not glucose?

, BIOL 3110 Final Exam Questions and
Answers (Solved Papers)
a. Mutation of the Repressor binding site so Repressor binds
tighter.
b. Mutation of the translation initiation codon to a stop codon.
c. Mutation of the translation initiation codon to a Leu codon.
d. Mutation of the Catabolite activator protein (CAP) binding
site so CAP does not
bind.
e. Mutation of the -35 region to change the sequence to
match consensus. - Correct Answers ✅e. Mutation of the -
35 region to change the sequence to match consensus.


Which of the following statements about E. coli RNA
polymerase is FALSE:


a. Binds DNA using the σ subunit.
b. Binds DNA using the α subunit.
c. Initiates RNA synthesis without a primer.
d. Uses the coding strand as a template.
e. Synthesizes RNA in the 5' to 3' direction. - Correct
Answers ✅d. Uses the coding strand as a template.


Which of the following statements about transcription in E.
coli is TRUE: