1|Page
BIO 340 MIDTERM EXAM NEWEST 2025/ 2026
COMPLETE QUESTIONS AND ANSWERS WITH
RATIONALES (VERIFIED ANSWERS) |ALREADY
GRADED A+|| LATEST EXAM!!!
A bacterial enzyme that cuts dna within a short, specific
sequence and is used in many applications by the
molecular biologist is known as: - answer-restriction
enzymes
Which of the following is not needed in order to amplify a
dna fragment by pcr - answer-knowledge of the complete
nucleotide sequence of the fragment to be amplified
Which of the following best describes the complete
sequence of steps occurring every cycle of pcr? - answer-
the primers hybridize to the target dna
Which is the function of dideoxynucelotides in sanger dna
sequencing? - answer-they stop synthesis at a specific
site, so the base at that site can be determined
An amino acid residues that is not part of the active site of
an enzyme and does not interact with the ligand is
nevertheless ciritical for ligand binding and is highly
conserved. How could this be explained? - answer-all
above
, 2|Page
The two chromosomes in each of the 22 homologous pairs
in our cells - answer-show identical banding patterns after
staining
What are the three functional elements required for
replication and stable inheritance of chromosome -
answer-origins of replication, centromeres, telomeres
In which phase of the eukaryotic cell cycle is the dna in its
most highly condensed form? - answer-m phase
Which of the following is true regarding heterochromatin in
a typical mammalian cell? - answer-heterochromatin is
characterized by its high degree of compaction
In the beads on a string model, the bead is made up of: -
answer-8 histone proteins
The chromatin remodeling complexes play an important
role in chromatin regulation in the nucleus. They.. -
answer-all of the above.
- can slide nucleosome on dna
-have atpase activity
BIO 340 MIDTERM EXAM NEWEST 2025/ 2026
COMPLETE QUESTIONS AND ANSWERS WITH
RATIONALES (VERIFIED ANSWERS) |ALREADY
GRADED A+|| LATEST EXAM!!!
A bacterial enzyme that cuts dna within a short, specific
sequence and is used in many applications by the
molecular biologist is known as: - answer-restriction
enzymes
Which of the following is not needed in order to amplify a
dna fragment by pcr - answer-knowledge of the complete
nucleotide sequence of the fragment to be amplified
Which of the following best describes the complete
sequence of steps occurring every cycle of pcr? - answer-
the primers hybridize to the target dna
Which is the function of dideoxynucelotides in sanger dna
sequencing? - answer-they stop synthesis at a specific
site, so the base at that site can be determined
An amino acid residues that is not part of the active site of
an enzyme and does not interact with the ligand is
nevertheless ciritical for ligand binding and is highly
conserved. How could this be explained? - answer-all
above
, 2|Page
The two chromosomes in each of the 22 homologous pairs
in our cells - answer-show identical banding patterns after
staining
What are the three functional elements required for
replication and stable inheritance of chromosome -
answer-origins of replication, centromeres, telomeres
In which phase of the eukaryotic cell cycle is the dna in its
most highly condensed form? - answer-m phase
Which of the following is true regarding heterochromatin in
a typical mammalian cell? - answer-heterochromatin is
characterized by its high degree of compaction
In the beads on a string model, the bead is made up of: -
answer-8 histone proteins
The chromatin remodeling complexes play an important
role in chromatin regulation in the nucleus. They.. -
answer-all of the above.
- can slide nucleosome on dna
-have atpase activity
