LATEST CHT EXAM COMPLETE STUDY
GUIDE||QUESTIONS AND WELL
VERIFIED ANSWERS (100%CORRECT))
||GRADED A+!!LATEST EXAM UPDATE
(2025-2026)
It is possible to convert the plasma
into serum by recalcifying the plasma and allowing coagulation to occur. -
CORRECTANSWERCalcium is added to the plasma to form a complex with the citrate
present in the anti-coagulant. This restores a normal
ionized calcium level allowing clotting to occur.
Polyspecific antiserum
can be______ with lymphocytes to determine if there is a single antibody or multiple
antibodies. -CORRECTANSWERabsorbed
Another application of lymphocyte
absorption is to use the patient's own cells to remove_____. Absorption can be
performed
at cold or warm temperatures depending on the type of antibody. -
CORRECTANSWERautoantibody
_________ are typically
cold reacting (4oC) and________are typically warm reacting (22-37oC). -
CORRECTANSWERAutoantibodies
B cell antibodies
Patient serum may contain autoantibodies and/or IgM antibodies that may interfere the
binding of
anti-HLA IgG antibodies to the antigen. In complement dependent assays (CDC), IgM
antibodies
can activate complement; potentially leading to ___________. -
CORRECTANSWERfalse positive reactivity.
DTT -CORRECTANSWERdithiothreitol, also known as Cleland's Reagent
DTT inactivates IgM molecules by cleaving the _________ in the
pentamer structure. -CORRECTANSWERdisulphide bonds
Heat inactivation works by cleaving the disulphide bonds in the
pentamer structure, but is less specific than _____. -CORRECTANSWERDTT.
GUIDE||QUESTIONS AND WELL
VERIFIED ANSWERS (100%CORRECT))
||GRADED A+!!LATEST EXAM UPDATE
(2025-2026)
It is possible to convert the plasma
into serum by recalcifying the plasma and allowing coagulation to occur. -
CORRECTANSWERCalcium is added to the plasma to form a complex with the citrate
present in the anti-coagulant. This restores a normal
ionized calcium level allowing clotting to occur.
Polyspecific antiserum
can be______ with lymphocytes to determine if there is a single antibody or multiple
antibodies. -CORRECTANSWERabsorbed
Another application of lymphocyte
absorption is to use the patient's own cells to remove_____. Absorption can be
performed
at cold or warm temperatures depending on the type of antibody. -
CORRECTANSWERautoantibody
_________ are typically
cold reacting (4oC) and________are typically warm reacting (22-37oC). -
CORRECTANSWERAutoantibodies
B cell antibodies
Patient serum may contain autoantibodies and/or IgM antibodies that may interfere the
binding of
anti-HLA IgG antibodies to the antigen. In complement dependent assays (CDC), IgM
antibodies
can activate complement; potentially leading to ___________. -
CORRECTANSWERfalse positive reactivity.
DTT -CORRECTANSWERdithiothreitol, also known as Cleland's Reagent
DTT inactivates IgM molecules by cleaving the _________ in the
pentamer structure. -CORRECTANSWERdisulphide bonds
Heat inactivation works by cleaving the disulphide bonds in the
pentamer structure, but is less specific than _____. -CORRECTANSWERDTT.
